Transmission imaging on a scintillator in a scanning electron microscope

Zuidema, Wilco; Kruit, P.

doi:10.1016/j.ultramic.2020.113055

Cited by 12 publications

(7 citation statements)

References 20 publications

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“…The primary beams and detected electrons are separated by a magnetic beam splitter. (B) Delft Multibeam (FAST‐EM) 33,174 . Instead of secondary electron detection, this multibeam system detects transmitted electrons via conversion to a light signal by a scintillator located directly under the sample…”

Section: Imaging Of Larger Biological Volumesmentioning

confidence: 99%

“…(B) Delft Multibeam (FAST-EM). 33,174 Instead of secondary electron detection, this multibeam system detects transmitted electrons via conversion to a light signal by a scintillator located directly under the sample loading and imaging for extended periods. Together, these innovations allow 40× faster imaging than conventional TEM.…”

Section: Multiple Cameras: Temca and Autotemmentioning

confidence: 99%

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How innovations in methodology offer new prospects for volume electron microscopy

Kievits

Lane

Carroll

et al. 2022

Journal of Microscopy

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Detailed knowledge of biological structure has been key in understanding biology at several levels of organisation, from organs to cells and proteins. Volume electron microscopy (volume EM) provides high resolution 3D structural information about tissues on the nanometre scale. However, the throughput rate of conventional electron microscopes has limited the volume size and number of samples that can be imaged. Recent improvements in methodology are currently driving a revolution in volume EM, making possible the structural imaging of whole organs and small organisms. In turn, these recent developments in image acquisition have created or stressed bottlenecks in other parts of the pipeline, like sample preparation, image analysis and data management. While the progress in image analysis is stunning due to the advent of automatic segmentation and server-based annotation tools, several challenges remain. Here we discuss recent trends in volume EM, emerging methods for increasing throughput and implications for sample preparation, image analysis and data management.

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Section: Imaging Of Larger Biological Volumesmentioning

confidence: 99%

Section: Multiple Cameras: Temca and Autotemmentioning

confidence: 99%

How innovations in methodology offer new prospects for volume electron microscopy

Kievits

Lane

Carroll

et al. 2022

Journal of Microscopy

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show abstract

“…Zuidema & Kruit (2020) demonstrated transmission imaging with a scintillator in an SEM which offers a way to separate the individual signals. It may also be preferred for imaging thin biological sections as it can yield better signal to noise and improved dynamic range [4].…”

mentioning

confidence: 99%

Characterization and Optimization of OSTEM; A Novel Detection Method for Single- and Multi-Beam Scanning Electron Microscopy

Kievits

Fermie²,

Duinkerken

et al. 2022

Microscopy and Microanalysis

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Recent developments in large scale and volume electron microscopy (EM) have revolutionized the understanding of biological systems across different length scales. However, the inherent low throughput of electron microscopes remains a major bottleneck for further progress. This is a result of a trade-off between acquisition speed and sufficient image quality for reconstruction and biological interpretation.

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“…Considering a typically large volume of biological material, for instance (0.25 mm) 3 , with slices of 40nm thickness, one would have to produce 6250 sections. Producing and collecting these sections would require roughly 20h with any of the techniques discussed above.…”

mentioning

confidence: 99%

A High-throughput Electron Microscopy Workflow and its Applications in Life Sciences

Ridolfi¹,

Niessen²,

Cioaba

et al. 2022

Microscopy and Microanalysis

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Transmission imaging on a scintillator in a scanning electron microscope

Cited by 12 publications

References 20 publications

How innovations in methodology offer new prospects for volume electron microscopy

How innovations in methodology offer new prospects for volume electron microscopy

Characterization and Optimization of OSTEM; A Novel Detection Method for Single- and Multi-Beam Scanning Electron Microscopy

A High-throughput Electron Microscopy Workflow and its Applications in Life Sciences

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