Transmission genetics of the somatic hybridization process in Nicotiana

Gleba, Yu. Yu.; Kolesnik, N. N.; Meshkene, I. V.; Cherep, N. N.; Parokonny, A. S.

doi:10.1007/bf00272881

Cited by 52 publications

(2 citation statements)

References 17 publications

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“…(1991) who, using micro-manipulator techniques coupled with, in the former report, light-bleached shoot culture protoplasts and, in the latter, a dual fluorescent system, selected hybrids after three to seven days culture . The selection and characterisation of regenerated (rather than at earlier stages of culture), presumptive somatic hybrid material has previously been undertaken by such methodology as intermediate morphology , isozyme markers (Gleba et al ., 1984) and restriction fragment length polymorphisms (Pehu et al ., 1989) . RFLP approaches were further refined, with ribosomal DNA probes used for somatic hybrid identification (Pental et al ., 1988 ;Rosen et al ., 1988) and species-specific sequences of DNA used to analyze somatic fusions of S. brevidens and S. tuberosum (Pehu et al ., 1990) .…”

Section: Asymmetric Strategiesmentioning

confidence: 99%

The integration of protoplast fusion-derived material into a potato breeding programme — a review of progress and problems

Millam¹,

Payne²,

Mackay³

1995

Euphytica

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This paper reviews investigations into the application of protoplast fusion to the genetic and agronomic improvement of potato . Fusion studies involving Solanum tuberosum are reviewed under the categories of : fusion with wild relatives, dihaploid fusion and asymmetric strategies . The selection and characterisation of putative somatic hybrid material is identified as a critical stage in the process and certain specific aspects of this technology are identified . Future prospects for the wider uptake and integration of these techniques into breeding programmes are also discussed .

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Section: Asymmetric Strategiesmentioning

confidence: 99%

The integration of protoplast fusion-derived material into a potato breeding programme — a review of progress and problems

Millam¹,

Payne²,

Mackay³

1995

Euphytica

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“…Successful regeneration of plants from cell fusion has been reported from about 50 interspecific and intergeneric protoplast combinations. Most somatic hybrids produced between N. tabacum and wild species in the genus Nicotiana were asymmetric, while only a few symmetric somatic hybrids have been obtained (Carlson et al, 1972;Douglas et al, 1981;Gleba et al, 1984;Bates, 1990;Sproule et al, 1991;Hagimori et al, 1993;Donaldson et al, 1995;Ilcheva et al, 2000). Using cell fusion, we have discovered a rare symmetric somatic hybrid between Nicotiana tabacum and N. glauca, which, to date, is the first symmetric interspecific hybrid of tobacco bred by cell fusion, as identified by random amplified polymorphic DNA (RAPD), SCAR and morphological characterization.…”

Section: Introductionmentioning

confidence: 98%

Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid betweenN. tabacumandN. glauca

Sun¹,

Xue²,

Zhang³

et al. 2007

Plant Biosystems - An International Journal Dealing with all As

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By using the protoplast fusion technique, we have obtained 44 regenerated plants, phenotypically different and distinct from their parents, among which we have identified a fertile symmetric somatic hybrid, designated as TG-32, between N. tabacum var. Gexin No.1 and N. glauca. The morphology, fertility, chromosome number and nuclear constitution of the somatic hybrid have been studied in detail. Unlike other asymmetric interspecific somatic hybrids, the chromosome number of the symmetric somatic hybrid is 72, equal to the sum of chromosomes of both parents. The TG-32 plant has flowers similar to those of N. tabacum, but with petals similar to those of N. glauca. Interestingly the offspring of TG-32 vary in seed production ability with temperature, and produce more seeds under a relatively low temperature. Two SCAR markers were used to evaluate genetic variability and structure. The hybrid amplified the expected fragment, but the parents showed only one of two markers. This experimental result supports the hypothesis of the co-existence of two parental genomes in the somatic hybrid.

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Eggplant

Rajam

Rotino²,

Sihachakr

et al. 2008

Compendium of Transgenic Crop Plants

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Eggplant ( Solanum melongena L.) is one of the important vegetable crops cultivated in various tropical and temperate parts of the world. There is a wide genetic diversity in the cultivated as well as wild species of eggplant. Although, the cultivated eggplant varieties are susceptible to various diseases and pests as well as abiotic stresses, the wild relatives of eggplant are good source of resistance against biotic and abiotic stresses. Therefore, attempts have been made to introgress these agronomically important traits from wild species to cultivated varieties of eggplant, but with limited success. Nevertheless, eggplant is quite amenable for plant regeneration through cell and tissue culture. Haploidisation, somatic hybridization and gene transfer methods have been well established for this crop. The beneficial traits like resistance to bacterial and fungal wilts have successfully been transferred from wild species to cultivated eggplants by protoplast fusion. However, the development of molecular markers and transgenic eggplants with novel traits is in infancy. Eggplant transgenics for insect resistance, abiotic stress tolerance, and parthenocarpy have been accomplished. More work is needed in this direction to produce eggplant transgenics for other agronomic traits, including disease resistance, nematode resistance, quality and shelf‐life of fruits. Modern biotechnological tools like RNA interference (RNAi) are useful to achieve these goals. The initiation of complete genome sequencing of eggplant is required to discover several useful genes, which can be introduced into eggplant and other crop species for genetic improvement. This chapter reviews the various aspects of eggplant biology, particularly the developments in eggplant biotechnology and future projections.

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Transmission genetics of the somatic hybridization process in Nicotiana

Cited by 52 publications

References 17 publications

The integration of protoplast fusion-derived material into a potato breeding programme — a review of progress and problems

The integration of protoplast fusion-derived material into a potato breeding programme — a review of progress and problems

Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid betweenN. tabacumandN. glauca

Eggplant

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