Transmission Electron Microscopic Analysis Showing Structural Changes to Bacterial Cells Treated with Electrolyzed Water and an Acidic Sanitizer

Feliciano, Lizanel; Lee, Jae-Sung; Pascall, Melvin A.

doi:10.1111/j.1750-3841.2012.02633.x

Cited by 32 publications

(17 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Close examination of both images, Salmonella Typhimurium SL 1344 treated with LB-spent culture supernatant and our C. sakazakii treated with Narine-CCFE, showed the same altered cell envelope. Feliciano et al (2012) reported that inactivated bacterial cells showed a less defined envelope along with a rugged cell wall, suggesting that inactivated cells appeared due to reduced integrity of the cell envelope and aggregation of cytoplasmic components.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Cronobacter sakazakii by Lactobacillus acidophilus n.v. Er2 317/402

Charchoghlyan¹,

Kwon²,

Hwang³

et al. 2016

Korean Journal for Food Science of Animal Resources

View full text Add to dashboard Cite

Lactobacillus acidophilus n.v. Er2 317/402 strain Narine is known as a health beneficial functional probiotic culture and supplementary source of nutrition for newborns. In this study, in vitro antimicrobial activities of Narine-lyophilized (Narine-L), Narine-heat treated (Narine-HT), and Narine crude cell-free extract (Narine-CCFE) were evaluated against pathogen Cronobacter sakazakii (C. sakazakii) in agar as well as in a reconstituted powdered infant formula (RPIF) model. Inhibition zones of 30 mg Narine-L and Narine-HT were both 150 U, whereas inhibition zone of 30 mg Narine-CCFE was 200 U. Narine-L (1 g) and Narine-HT (1 g) were added to 10 mL of artificially contaminated RPIF, respectively, containing 100 μL of C. sakazakii (1.62×108 colony forming unit (CFU)/mL). After treatment with Narine-L and Narine-HT for 3 h and 6 h at 37℃, less than ≤107 CFU/mL of C. sakazakii was detected in RPIF. Without Narine-L and Narine-HT treatment, the population of C. sakazakii increased up to 5.36×109 CFU/mL after 6 h. Examination by transmission electron microscopy confirmed C. sakazakii cells were damaged by Narine-CCFE. Thus, employing Narine culture as a natural and safe bio-preservative may protect infants from C. sakazakii.

show abstract

Section: Resultsmentioning

confidence: 99%

Inhibition of Cronobacter sakazakii by Lactobacillus acidophilus n.v. Er2 317/402

Charchoghlyan¹,

Kwon²,

Hwang³

et al. 2016

Korean Journal for Food Science of Animal Resources

View full text Add to dashboard Cite

show abstract

“…Moreover, our results were also supported by Skrivanova et al (2006), who found that AEW was more effective against planktonic Gram-positive bacteria than planktonic Gram-negative bacteria. One explanation of these phenomena is that the transport of ions across the cell membrane of Gram-positive bacteria is more vulnerable to interference (Skrivanova et al, 2006; Feliciano et al, 2012; Chen et al, 2014). In addition, this apparent discrepancy could partly be explained by the different structural composition of EPS in different biofilms.…”

Section: Discussionmentioning

confidence: 99%

Removal of Foodborne Pathogen Biofilms by Acidic Electrolyzed Water

et al. 2017

View full text Add to dashboard Cite

Biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (EPS), are difficult to remove in food production facilities. In this study, the use of acidic electrolyzed water (AEW) to remove foodborne pathogen biofilms was evaluated. We used a green fluorescent protein-tagged Escherichia coli for monitoring the efficiency of AEW for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm disappeared rapidly and the population of biofilm cells was reduced by more than 67%. Additionally, AEW triggered EPS disruption, as indicated by the deformation of the carbohydrate C-O-C bond and deformation of the aromatic rings in the amino acids tyrosine and phenylalanine. These deformations were identified by EPS chemical analysis and Raman spectroscopic analysis. Scanning electron microscopy (SEM) images confirmed that the breakup and detachment of biofilm were enhanced after AEW treatment. Further, AEW also eradicated biofilms formed by both Gram-negative bacteria (Vibrio parahaemolyticus) and Gram-positive bacteria (Listeria monocytogenes) and was observed to inactivate the detached cells which are a potential source of secondary pollution. This study demonstrates that AEW could be a reliable foodborne pathogen biofilm disrupter and an eco-friendly alternative to sanitizers traditionally used in the food industry.

show abstract

“…AEW is commonly used in the agricultural and medical fields as a disinfectant for farm and food hygiene (Fabrizio and Cutter, 2004;Northcutt et al, 2007;Guentzel et al, 2008;Cao et al, 2009;Park et al, 2009;Lu et al, 2010;Rahman et al, 2010Rahman et al, , 2011. It is also widely used for the disinfection of medical instruments such as dialyzers (Tanaka et al, 2000), endoscopes (Lee et al, 2004), and dentures (Nagamatsu et al, 2001) because of its potent antimicrobial potential (Kiura et al, 2002;Sharma and Demirci, 2003;Nisola et al, 2011;Rodriguez-Garcia et al, 2011;Feliciano et al, 2012). AEW has potent oxidative power in relation to its antimicrobial potential.…”

Section: Introductionmentioning

confidence: 99%

Involvement of reactive oxygen species in the cytotoxic effect of acid-electrolyzed water

2015

View full text Add to dashboard Cite

-Acid-electrolyzed water (AEW) is commonly used as a disinfectant in the agricultural and medical fields. Although several studies have been conducted to examine its toxicity in vitro and in vivo, the cytotoxic mechanism of AEW has never been verified. The purpose of the present study was to elucidate the underlying mechanism by which AEW exerts its in vitro cytotoxic effect. Mouse fibroblasts treated with AEW experienced dilution rate-dependent cytotoxic effects in the 100% confluent phase as well as in the mitotic phase. The levels of intracellular reactive oxygen species (ROS) increased significantly in fully-confluent cells treated with undiluted and four times diluted AEW. In both of these treatments, cytotoxicity was also observed. It is thus concluded that the in vitro cytotoxicity of AEW is attributable to increased intracellular ROS. Additionally, the ROS responsible for these effects appears to be, at least in part, hydroxyl radical because the increase in intracellular ROS was attenuated by post-treatment with dimethyl sulfoxide, a hydroxyl radical scavenger, and with the antioxidant polyphenol, proanthocyanidin.

show abstract

Transmission Electron Microscopic Analysis Showing Structural Changes to Bacterial Cells Treated with Electrolyzed Water and an Acidic Sanitizer

Cited by 32 publications

References 34 publications

Inhibition of Cronobacter sakazakii by Lactobacillus acidophilus n.v. Er2 317/402

Inhibition of Cronobacter sakazakii by Lactobacillus acidophilus n.v. Er2 317/402

Removal of Foodborne Pathogen Biofilms by Acidic Electrolyzed Water

Involvement of reactive oxygen species in the cytotoxic effect of acid-electrolyzed water

Contact Info

Product

Resources

About