Transmission and scanning electron microscopy confirm that bone microstructure is similar in osteopenic and osteoporotic patients

Abstract

Amaç: Daha önce "ışık mikroskopi çalışması" ile elde edilen "Femur boyun kırığı olan osteopenik ve osteoporotik hastalarda kemik mikromimarisi benzerdir" bulgusunu ki, önemli ve yenidir, teyit etmek amaçlandı. Hastalar ve yöntemler: Düşük enerjili travma sonrası femur proksimal uç kırığı nedeniyle başvuran 14 hasta (5 erkek, 9 kadın) çalışmaya alındı (ışık mikroskopi çalışmasına katılan hastalar). Hastalar kemik mineral yoğunluğu (KMY) ölçümlerine göre osteopenik (n=7, ort. yaş 69 yıl; dağılım 63-74 yıl) ve osteoporotik (n=7, ort. yaş 74.1 yıl; dağılım 67-78 yıl) olarak iki gruba ayrıldı. Parsiyel kalça artroplastisi sırasında endoprotez uygulanan hastalardan kortikal ve trabeküler kemik örnekleri alındı ve alınan bu örnekler, daha gelişmiş ve yüksek çözünürlüklü transmisyon ve taramalı elektron mikroskopisiyle incelendi. Bulgular: Ortalama kortikal kemik kalınlığı osteopenik grupta 3622.14 mm; osteoporotik grupta ise 2323.14 mm idi (p<0.005). Transmisyon elektron mikroskopisi ve taramalı elektron mikroskopisi değerlendirmesinde her iki grupta da benzer bulgular saptandı. Sonuç: Gruplar arasında kortikal kalınlık açısından anlamlı farklılık bulunmuş olsa da, transmisyon ve taramalı elektron mikroskopisi, daha önceki ışık mikroskopi çalışma-sında olduğu gibi, kemik mikromimarisi düşük enerjili femur boyun kırığı olan osteopenik ve osteoporotik hastalarda benzer özellikler taşıdığını teyit etmiştir.Anahtar sözcükler: Kemik mikromimarisi; osteopeni; taramalı elektron mikroskopisi; osteoporoz; transmisyon elektron mikroskopisi. Objectives:The objective was to confirm the finding of "Bone microstructure is similar in osteopenic and osteoporotic patients with femoral neck fracture." obtained in previous "light microscopy study", which was new and important data. Patients and methods: Fourteen patients (5 males, 9 females) who were admitted with proximal femoral fracture following low energy trauma (patients who participated in the light microscopy study) were included. The patients were divided into two groups based on the bone mineral density (BMD) measurement, including osteopenic group (n=7, mean age 69 years; range 63 to 74 years) and osteoporotic group (n=7, mean age 74.1 years; range 67 to 78 years). Cortical and trabecular bone samples were taken from the patients who underwent endoprosthesis during partial hip arthroplasty and these samples were analyzed using transmission electron microscopy and scanning electron microscopy evaluations which are more sophisticated higher resolution techniques. Results: The mean cortical bone thickness was 3622.14 mm in osteopenic group and 2323.14 mm in osteoporotic group (p<0.005). Transmission electron microscopy and scanning electron microscopy evaluations revealed similar findings for both groups. Conclusion: Although a significant difference in cortical thickness was found between the groups, transmission and scanning electron microscopy confirmed that bone microstructure shared similar characteristics in osteopenic and osteoporotic patients with low-energy femoral neck ...

A comparison of proximal femoral locking plate versus 95-degree angled blade plate in the treatment of reverse intertrochanteric fractures

“…The mean time to union was 17 weeks (range [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] in the PFLP group ( Figure 1) and 18 weeks (range 16-32) in the ABP group (Figure 2). The mean ASA score was 2.7 and 3.1 in the PFLP and ABP groups, respectively.…”

“…osteoporotic fractures. [28] However, further studies consisting of homogeneous subgroup types with similar degree of osteoporotic bone can reveal more accurate results on indications and effectiveness of PFLP and ABP.…”

Analysis of musculoskeletal dysmorphic abnormalities of 20 fetuses

“…One fetus diagnosed as PLH [14] had generalized defective mineralization of the bones including the skull and multiple fractures due to low activity of serum alkaline phosphatase enzyme. In parental history, consanguineous marriage and one more baby with diagnosed PLH were noted (Figure 9).…”

Do atypical femoral fractures in patients with prolonged alendronate treatment heal?

“…[1,2] There are reports indicating that long-term alendronate use may cause an increased susceptibility to fractures. [3,4] Also, recent reports have suggested that a potential complication may be delayed union after atypical femoral fracture stabilization in patients taking long-term bisphosphonate.…”