Transmission and detection of monkeypox virus in saliva: Implications for dental practice and public health

Yang, Xi; Tang, Guoyao; Shi, Linjun; Xu, Feng

doi:10.1016/j.jds.2022.10.016

Cited by 3 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be easily self-collected and sample collection does not require expertise [ 59 ]. Saliva specimens can be collected in community and household areas where negative ventilation chambers are not available, minimizing the exposure of healthcare workers to the virus and the risk of respiratory cross-infection [ 60 ]. This may be relevant to increasing the safety of frontline healthcare workers while analyzing evidence for the contribution of the respiratory route to the transmission of mpox is achieved.…”

Section: Mpox Salivary Diagnostics and Omics Perspectives On Mpox Res...mentioning

confidence: 99%

“…There are several potential pathways for MPXV to be present in saliva, including infection of minor and major salivary glands, oral mucosal cells from macules and ulcers, lymph from maxillofacial and cervical lymphadenopathy, and gingival crevicular fluid containing serum-derived proteins [ 60 ]. Moreover, MPXV may not be positive in other bodily fluids and swab samples, portraying saliva as plausible and highly sensitive samples, especially when collected three or four days after the onset of symptoms [ 60 ]. Although recent advances have occurred in antibody tests for viral diseases, the previous cross-reactivity with antibodies induced by prior smallpox vaccination can disrupt the expected accuracy for MPXV antibody tests [ 35 , 40 , 66 ].…”

Section: Mpox Salivary Diagnostics and Omics Perspectives On Mpox Res...mentioning

confidence: 99%

See 1 more Smart Citation

Oral Infection, Oral Pathology and Salivary Diagnostics of Mpox Disease: Relevance in Dentistry and OMICs Perspectives

Garcia-Junior,

Andrade,

Guevara-Vega

et al. 2023

IJMS

View full text Add to dashboard Cite

In this narrative review, we aim to point out the close relationship between mpox virus (MPXV) infection and the role of saliva as a diagnostic tool for mpox, considering the current molecular approach and in the perspective of OMICs application. The MPXV uses the host cell’s rough endoplasmic reticulum, ribosomes, and cytoplasmic proteins to replicate its genome and synthesize virions for cellular exit. The presence of oral mucosa lesions associated with mpox infection is one of the first signs of infection; however, current diagnostic tools find it difficult to detect the virus before the rashes begin. MPXV transmission occurs through direct contact with an infected lesion and infected body fluids, including saliva, presenting a potential use of this fluid for diagnostic purposes. Currently available diagnostic tests for MPXV detection are performed either by real-time quantitative PCR (RT-qPCR) or ELISA, which presents several limitations since they are invasive tests. Despite current clinical trials with restricted sample size, MPXV DNA was detected in saliva with a sensitivity of 85%–100%. In this context, the application of transcriptomics, metabolomics, lipidomics, or proteomics analyses coupled with saliva can identify novel disease biomarkers. Thus, it is important to note that the identification and quantification of salivary DNA, RNA, lipid, protein, and metabolite can provide novel non-invasive biomarkers through the use of OMICs platforms aiding in the early detection and diagnosis of MPXV infection. Untargeted mass spectrometry (MS)-based proteomics reveals that some proteins also expressed in saliva were detected with greater expression differences in blood plasma when comparing mpox patients and healthy subjects, suggesting a promising alternative to be applied in screening or diagnostic platforms for mpox salivary diagnostics coupled to OMICs.

show abstract

Section: Mpox Salivary Diagnostics and Omics Perspectives On Mpox Res...mentioning

confidence: 99%

Section: Mpox Salivary Diagnostics and Omics Perspectives On Mpox Res...mentioning

confidence: 99%

Oral Infection, Oral Pathology and Salivary Diagnostics of Mpox Disease: Relevance in Dentistry and OMICs Perspectives

Garcia-Junior,

Andrade,

Guevara-Vega

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…If universal health policy is adopted globally, it will provide a better platform for the study and innovation of developing zoonotic diseases, as well as aid in MPX prevention. [71][72][73]…”

Section: Public Health Implicationmentioning

confidence: 99%

An International Outburst of New Form of Monkeypox Virus

Dobhal¹,

Ghildiyal²,

Ansori³

et al. 2022

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

A new strain of the old pandemic, Monkeypox (MPX), has emerged with a more complicated clinical appearance. It is a source of relief that the fatality rate in the new monkeypox is lower, but communicability is higher. This infection’s diagnosis and therapy are still challenging and unknown. Researchers are reporting increased human-to-human transmission in the modified version of MPX. There have been several reports of the updated version of monkeypox in the European and American areas. Brazil, Colombia, France, Spain, Germany, Peru, the United Kingdom, and the United States of America have recorded over three thousand new cases of monkeypox through October 2022. Few antiviral medicines and vaccines are available on the market, making treatment of this condition difficult. MPX was previously declared an epidemic disease, but ignorance about it can bring devastation in the shape of the next pandemic-like COVID-19. This review aims to assess the virology, transmission, diagnosis, and therapy of MPX.

show abstract

“…Recently, we and other authors propose that dentists and maxillofacial specialists should keep an eye out for oral and maxillofacial manifestations of monkeypox infection and protective measures. 2 , 3 , 4 , 5 For dentists and oral medicine specialists, special attention is thereupon focused on the use of saliva as a sampling material for MPXV test. 2 Whether saliva may emerge as viable sample for sequential monitoring of viral load is not described.…”

mentioning

confidence: 99%

“… 2 , 3 , 4 , 5 For dentists and oral medicine specialists, special attention is thereupon focused on the use of saliva as a sampling material for MPXV test. 2 Whether saliva may emerge as viable sample for sequential monitoring of viral load is not described. This paper attempted to describe what saliva can tell us in the transmission and infection of MPXV to better understand the possible role in monitoring viral load.…”

mentioning

confidence: 99%

Transmission and detection of monkeypox virus in saliva (part II): Implications for sequential monitoring of viral load

Yang

Xiao

Liao

et al. 2023

Journal of Dental Sciences

Self Cite

View full text Add to dashboard Cite

Transmission and detection of monkeypox virus in saliva: Implications for dental practice and public health

Cited by 3 publications

References 10 publications

Oral Infection, Oral Pathology and Salivary Diagnostics of Mpox Disease: Relevance in Dentistry and OMICs Perspectives

Oral Infection, Oral Pathology and Salivary Diagnostics of Mpox Disease: Relevance in Dentistry and OMICs Perspectives

An International Outburst of New Form of Monkeypox Virus

Transmission and detection of monkeypox virus in saliva (part II): Implications for sequential monitoring of viral load

Contact Info

Product

Resources

About