Transmissible Plasmid Coding Early Enzymes of Naphthalene Oxidation in Pseudomonas putida

Bacterial strain M213 was isolated from a fuel oil-contaminated soil in Idaho, USA, by growth on naphthalene as a sole source of carbon, and was identified as Rhodococcus opacus M213 by 16S rDNA sequence analysis and growth on substrates characteristic of this species. M213 was screened for growth on a variety of aromatic hydrocarbons, and growth was observed only on simple 1 and 2 ring compounds. No growth or poor growth was observed with chlorinated aromatic compounds such as 2,4-dichlorophenol and chlorobenzoates. No growth was observed by M213 on salicylate, and M213 resting cells grown on naphthalene did not attack salicylate. In addition, no salicylate hydroxylase activity was detected in cell free lysates, suggesting a pathway for naphthalene catabolism that does not pass through salicylate. Enzyme assays indicated induction of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase on different substrates. Total DNA from M213 was screened for hybridization with a variety of genes encoding catechol dioxygenases, but hybridization was observed only with catA (encoding catechol 1,2-dioxygenase) from R. opacus 1CP and edoD (encoding catechol 2,3-dioxygenase) from Rhodococcus sp. I1. Plasmid analysis indicated the presence of two plasmids (pNUO1 and pNUO2). edoD hybridized to pNUO1, a very large (V750 kb) linear plasmid. ß

Section: Introductionmentioning

confidence: 99%

Characterization of the naphthalene-degrading bacterium, Rhodococcus opacus M213

2000

“…Plasmids NAH and pBS3 controlling degradation of naphthalene via the meta-pathway of catechol oxidation were used [1,2]. Plasmid pBS4 codes for the oxidation of naphthalene via gentisic acid [3].…”

Section: Methodsmentioning

confidence: 99%

“…Studies of naphthalene degradative plasmids [1][2][3][4][5] showed them to control various biochemical pathways of naphthalene oxidation (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Growth and plasmid-encoded naphthalene catabolism of Pseudomonas putida in batch culture

Boronin¹

1993

The growth characteristics of Pseudomonas putida plasmid-harbouring strains which catabolize naphthalene via various pathways in batch culture with naphthalene as the sole source of carbon and energy have been investigated. The strains under study were constructed using the host strain P. putida BS394 which contained various naphthalene degradation plasmids. The highest specific growth rate was ensured by the plasmids that control naphthalene catabolism through the meta-pathway of catechol oxidation. The strains metabolizing catechol via the ortho-pathway grew at a lower rate. The lowest growth rate was observed with strain BS291 harbouring plasmid pBS4 which controls naphthalene catabolism via the gentistic acid pathway. Various pathways of naphthalene catabolism appear to allow these strains to grow at various rates which should be taken into account when constructing efficient degraders of polycyclic aromatic compounds.

“…Both the naphthalene and salicylate utilizing phenotypes in P. putida G7 have been attributed to the NAH catabolic plasmid [5]. It was shown that the naphthalene catabolic genes are organized into two nah operons: the ¢rst operon encoding the conversion of naphthalene to salicylate (upper pathway), which includes genes nahABCDEF, and the second operon (nahGHIJK) encoding the oxidation of salicylate [6].…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narB) from Rhodococcus sp. NCIMB12038

Kulakov¹

2000

Rhodococcus sp. NCIMB112038 can utilize naphthalene as its sole carbon and energy source. The gene encoding cis-naphthalene dihydrodiol dehydrogenase (narB) of this strain has been cloned and sequenced. Expression of NCIMB12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in Escherichia coli cells. narB encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from Pseudomonas putida G7. Comparison of NarB with some putative cisdihydrodiol dehydrogenases from Rhodococcus species revealed significant differences between these proteins. NarB together with two other proteins forms a new group of cis-dihydrodiol dehydrogenases. ß