Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains

To, Thanh Long; Bernard, Serge; Bottreau, Elisabeth

doi:10.1016/s0923-2516(06)80112-3

Cited by 4 publications

(1 citation statement)

References 22 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The distribution of recombinant S antigen on the cell surface was determined by indirect immunofluorescence assay of cells inoculated with AdgpS at a multiplicity of 10 TCID~0/cell and fixed after 24 h in 0.1% paraformaldehyde for 30 min at 4 °C as described by To et al (1992). The cells were probed with a 1/100 dilution of two pools of monoclonal antibodies, directed against either the antigenic site A or site D of the S protein (Correa et al, 1988).…”

Section: The Sense Primer 5' T a G C T A G C C A C A C C A T G A A A mentioning

confidence: 99%

An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine

Callebaut

Enjuanes

Pensaert

1996

Journal of General Virology

View full text Add to dashboard Cite

show abstract

Section: The Sense Primer 5' T a G C T A G C C A C A C C A T G A A A mentioning

confidence: 99%

An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine

Callebaut

Enjuanes

Pensaert

1996

Journal of General Virology

View full text Add to dashboard Cite

show abstract

Expression and Immunogenicity of the Spike Glycoprotein of Porcine Respiratory Coronavirus Encoded in the E3 Region of Adenovirus

Callebaut

Pensaert

1995

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

The full length spike (S) gene of porcine respiratory coronavirus (PRCV) was inserted into the genome of human adenovirus type 5 downstream of the early transcription region 3 promoter. The recombinant virus replicated in cultures of the swine testicle ST cell line and directed the synthesis of S antigen to an amount of approximately 33 J.lg per 10 6 cells, as determined by ELISA. The antigen was cell-associated except in the late phase of the infection, when a low amount (4 J.lg per 10 6 cells) was released in the culture supernatant. The cell-associated antigen consisted of 2 polypeptides of 160 K and 175 K, respectively. The 160 K polypeptide comigrated with the authentic S' precursor from PRCV-infected cells. The 175 K polypeptide had the same mobility as the authentic mature S protein from PRCV-infected cells and from PRCV released in the supernatant. The extracellular recombinant antigen corresponded with the 175 K mature protein. Immunofluorescent staining gave evidence that some recombinant S protein was exposed on the cell surface; it also showed that the protein was recognized by conformation-specific anti-S monoclonal antibodies. Piglets, immunized oronasally with the recombinant adenovirus vector developed PRCV-neutralizing serum antibodies and were partially protected against PRCV -challenge.

show abstract

Immunohistochemistry of Transmissible Gastroenteritis Virus Antigens in Fixed Paraffin-Embedded Tissues

et al. 1996

View full text Add to dashboard Cite

Abstract. An immunohistochemistry technique was developed using fixed tissues to study the presence and location of transmissible gastroenteritis virus (TGEV) antigens in situ. Experimentally infected gnotobiotic and conventional pigs as well as pigs with natural TGEV infection were examined. The staining technique was based on detection of the major structural protein of TGEV, the nucleocapsid, by using a pool of 3 monoclonal antibodies. Formalin and periodate-lysine-paraformaldehyde (PLP)-fixed intestinal tissues from a gnotobiotic pig inoculated with virulent TGEV were used to determine optimal antibody concentrations and incubation times. The intestinal tissues remained in their respective fixatives for 6 months, and serial sections were removed at sequential times and embedded in paraffin blocks. PLP and 10% neutral buffered formalin were acceptable fixatives and preserved TGEV nucleocapsid antigenicity for up to 6 months. Formalin, in comparison with PLP as a fixative, was better for preserving original tissue morphology and provided better antigen detection. Conventional crossbred pigs were inoculated with virulent TGEV, and animals were euthanized on various postexposure days. Intestinal tissues were positive for TGEV nucleocapsid antigens on postexposure days 2, 4, and 8. The immunohistochemistry technique detected TGEV antigen in stored paraffin-embedded tissues from 14 naturally infected pigs previously confirmed as positive for TGEV using a direct immunofluorescence assay on intestinal mucosal smears, whereas 9 naturally infected pigs confirmed negative for TGEV antigen by the same immunofluorescence assay showed no staining consistent with the presence of TGEV antigen. Immunohistochemistry provides a method to detect TGEV and possibly other closely related coronaviruses such as porcine respiratory coronavirus in situ. A diagnostic test using the same fixed tissues processed for histopathology provides veterinary practitioners an alternative to delivering live pigs or refrigerated fresh intestinal samples containing infectious virus to a diagnostic laboratory. Investigators can utilize this technique to retrospectively screen fixed tissues for TGEV antigen.Transmissible gastroenteritis (TGE) virus (TGEV) belongs to the family Coronaviridae and causes enteritis in swine of all ages. Enteritis from TGEV results from destruction of villous enterocytes and villous atrophy of the small intestinal mucosa, especially within the jejunum and ileum.11 Clinical signs of TGE include anorexia, vomiting, diarrhea, and dehydration. Mortality in infected neonatal piglets approaches 100% because of severe diarrhea and dehydration.14 TGE annually costs the swine industry approximately $200 million through mortality and decreased production efficiency. ' During outbreaks of swine diarrhea, a presumptive diagnosis of TGEV can be made by evaluation of disease history and clinical signs. Histopathologic sections of formalin-fixed jejunum and ileum showing evidence of villous atrophy, although consistent with TGEV infect...

show abstract

Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains

Cited by 4 publications

References 22 publications

An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine

An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine

Expression and Immunogenicity of the Spike Glycoprotein of Porcine Respiratory Coronavirus Encoded in the E3 Region of Adenovirus

Immunohistochemistry of Transmissible Gastroenteritis Virus Antigens in Fixed Paraffin-Embedded Tissues

Contact Info

Product

Resources

About