Transmethylation reactions in bacterial lipids

Law, John H.; Zalkin, Howard; Kaneshiro, Tsuneo

doi:10.1016/0006-3002(63)90734-0

Cited by 157 publications

(43 citation statements)

References 29 publications

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“…5). During the late exponential and stationary phase, C,, OLYL increased to about 90% (W3110) and 60% (W3110[pGEc47dB), which is compatible with an increasing activity of cyclopropane fatty acid (CFA) synthetase with prolonged growth (Law et a]., 1963;Cronan et al, 1974).…”

Section: Induction Profile Of Alkb In E Coli W3110[pgec47]mentioning

confidence: 81%

“…The conversion of UFAs to cyclopropane derivatives is catalyzed by CFA synthetase, which acts at the phospholipid level (Law et al, 1963), and is normally activated when E. coli enters the stationary phase. The decrease in the conversion of C,,., to C,,:o,y, following induction of alkB, even in the stationary phase, is probably due to a lower CFA synthetase activity.…”

Section: Discussionmentioning

confidence: 99%

“…Induction of the alk genes in E. coli W3110[pGEc47] leads to changes in the relative amounts of fatty acids compared to those found in the wild-type host strain (Nieboer et al, 1993). A major change involves C,, , and C,, ocyo major unsaturated fatty acids in W3110, the interconversion of which is carried out by the cyclic fatty acid synthetase (Law et al, 1963). The sum of these two fatty acids is nearly constant in all W3110 recombinants (Nieboer et al, 1993).…”

Section: Induction Profile Of Alkb In E Coli W3110[pgec47]mentioning

confidence: 99%

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Overproduction of a Foreign Membrane Protein in Escherichia Coli Stimulates and Depends on Phospholipid Synthesis

Nieboer¹,

Vis²

1996

European Journal of Biochemistry

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When the Pseudomonas oleovorans alk system, consisting of the alkBFGHJKL and alkST genes, is expressed in Escherichia coli W3110, significant changes in phospholipid metabolism of the host are observed. A major role seems to be played by the cytoplasmic membrane protein alkane hydroxylase (AlkB), which is synthesized as up to 10-15% of the total protein in this strain [Nieboer, M., Kingma, J. & Witholt, B. (1993) The alkane oxidation system of Pseudomonas oleovorans: induction of the alk genes in Escherichia coli W311 O[pGEc47] affects membrane biogenesis and results in overexpression of alkane hydroxylase in a distinct cytoplasmic membrane subfraction, Mol. Microbiol. 8, 1039-10511. In the present paper, we have studied the link between synthesis of the membrane protein and the synthesis of phospholipids and fatty acids by examining the kinetics of these processes.Using [35S]methionine labeling, it was shown that induction of AlkB was maximal within 30-60 min after addition of inducer, when up to 15 % of all newly synthesized protein is AlkB. Phospholipid synthesis was followed by measuring the incorporation of 14C-labeled acetate and '3P-labeled phosphoric acid into phospholipids. Despite a negative effect of the inducer on the growth rate of W31?0[pGEc47], net phospholipid synthesis was significantly enhanced as a result of the expression of alkB. Synthesis of all three major phospholipids were stimulated to comparable extents by the induction of alkB. Induction did not increase "P incorporation into lipids in the control recombinant alk' strain which lacked alkB.Simultaneous with AlkB synthesis, the conversion of unsaturated 9-hexadecenoic acid (C,6 ,) into 9,lO-methylene hexadecanoic acid (C17 ocyc) was reduced in the alk' recombinant. Overall, these data show that the production of a foreign membrane protein in E. coli can engender a response of the phospholipid-synthesizing system of the host. In the absence of such a response, induction of the alk system would be much more toxic to the cells. Apparently, the increased phospholipid synthesis plays an important role in enabling the AlkB overproducing strain to grow.

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Section: Induction Profile Of Alkb In E Coli W3110[pgec47]mentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Induction Profile Of Alkb In E Coli W3110[pgec47]mentioning

confidence: 99%

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Overproduction of a Foreign Membrane Protein in Escherichia Coli Stimulates and Depends on Phospholipid Synthesis

Nieboer¹,

Vis²

1996

European Journal of Biochemistry

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“…Craven and Jeffrey (36) showed through x-ray studies that CPA-FAs have a similar shape and crystal structure to the corresponding monoenoic acids. In addition, phospholipids containing either unsaturated or CPA-FAs are similar in their solubility characteristics in polar solvents and in their ability to form stable micellar dispersions (37,38). Therefore, in many respects CPA-FAs have both similar physical properties and positional distribution as monounsaturated fatty acids in phospholipids.…”

Section: The Addition Of Methylene Group Takes Place On the Sn-1 Posimentioning

confidence: 99%

Characterization of Cyclopropane Fatty-acid Synthase fromSterculia foetida

Bao

Thelen²,

Bonaventure

et al. 2003

Journal of Biological Chemistry

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“…Lactobacillus arabinosus collected in the stationary phase consistently showed a higher percentage of saturated acid as compared to the organism collected during the logarithmic phase (Croom and McNeill, 1961). A similar effect was found in Escherichia coli by Marr and Ingraham (1962) and Law et al (1963); they noticed an increase in cyclopropane acid and a decrease in and C^g monoenoic acids as the culture aged. Phosphatidyl glycerol content of E. coli was much higher during the log phase than during the stationary phase.…”

Section: Influence Of Cultural Conditions On Bacterial Lipidssupporting

confidence: 79%

Fatty acids of Bacillus polymyxa and Bacillus stearothermophilus as influenced by temperature

Yao

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Transmethylation reactions in bacterial lipids

Cited by 157 publications

References 29 publications

Overproduction of a Foreign Membrane Protein in Escherichia Coli Stimulates and Depends on Phospholipid Synthesis

Overproduction of a Foreign Membrane Protein in Escherichia Coli Stimulates and Depends on Phospholipid Synthesis

Characterization of Cyclopropane Fatty-acid Synthase fromSterculia foetida

Fatty acids of Bacillus polymyxa and Bacillus stearothermophilus as influenced by temperature

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