Transmembrane routes of cationic liposome-mediated gene delivery using human throat epidermis cancer cells

Cui, Shaohui; Wang, Bing; Zhao, Yinan; Ding, Huiqin; Zhi, Defu; Zhang, Shubiao

doi:10.1007/s10529-013-1325-0

Cited by 18 publications

(16 citation statements)

References 12 publications

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“…Liposomes are nanovesicles composed of phospholipid bilayers that have various beneficial characteristics, such as good cell affinity, 7,8 targeting properties, [9][10][11] and sustained drug-release behavior. 12 Due to their favorable ability to encapsulate both hydrophobic and hydrophilic drugs, liposomes are widely used as carriers for bioactive drugs, including antineoplastic drugs, 13,14 antimicrobial drugs, 15 antiparasitic drugs, 16 hormonal drugs, 17 proteins, 18,19 and nucleic acid drugs 20,21 such as DNA [22][23][24] and siRNA. 25,26 Additionally, liposomes have the capacity to reduce the toxicity of these drugs.…”

Section: Introductionmentioning

confidence: 99%

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

Luo¹,

Yang²,

Yao³

et al. 2019

IJN

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Slp forms a crystalline array of proteins on the outermost envelope of bacteria and archaea with a molecular weight of 40-200 kDa. Slp can self-assemble on the surface of liposomes in a proper environment via electrostatic interactions, which could be employed to functionalize liposomes by forming Slp-coated liposomes for various applications. Among the molecular characteristics, the stability, adhesion, and immobilization of biomacromolecules are regarded as the most meaningful. Compared to plain liposomes, Slp-coated liposomes show excellent physicochemical and biological stabilities. Recently, Slp-coated liposomes were shown to specifically adhere to the gastrointestinal tract, which was attributed to the "ligand-receptor interaction" effect. Furthermore, Slp as a "bridge" can immobilize functional biomacromolecules on the surface of liposomes via protein fusion technology or intermolecular forces, endowing liposomes with beneficial functions. In view of these favorable features, Slp-coated liposomes are highly likely to be an ideal platform for drug delivery and biomedical uses. This review aims to provide a general framework for the structure and characteristics of Slp and the interactions between Slp and liposomes, to highlight the unique properties and drug delivery as well as the biomedical applications of the Slp-coated liposomes, and to discuss the ongoing challenges and perspectives.

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Section: Introductionmentioning

confidence: 99%

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

Luo¹,

Yang²,

Yao³

et al. 2019

IJN

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“…Other approaches, such as molecular beacons, etc., only contain one fluorophore per probe, which given the short 3΄-UTR, would limit labeled-mRNA brightness and thus the detection of mRNAs. Labeling of mRNA with MTRIPs, in contrast to covalent incorporation of fluorophores, does not significantly affect the behavior or localization of target mRNA and does not illicit cellular immune responses ( 27 , 29 – 32 ). Finally, this method does not require large sequence incorporations into the mRNA itself or co-expression with reporters, such as with the MS2 aptamer and MS2-like systems ( 21 ).…”

Section: Introductionmentioning

confidence: 99%

Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA–protein correlations at the level of single cells

Kirschman

Bhosle

Vanover

et al. 2017

Nucleic Acids Research

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The use of synthetic messenger ribonucleic acid (mRNA) to express specific proteins is a highly promising therapeutic and vaccine approach that avoids many safety issues associated with viral or DNA-based systems. However, in order to optimize mRNA designs and delivery, technology advancements are required to study fundamental mechanisms of mRNA uptake and localization at the single-cell and tissue level. Here, we present a single RNA sensitive fluorescent labeling method which allows us to label and visualize synthetic mRNA without significantly affecting function. This approach enabled single cell characterization of mRNA uptake and release kinetics from endocytic compartments, the measurement of mRNA/protein correlations, and motivated the investigation of mRNA induced cellular stress, all important mechanisms influencing protein production. In addition, we demonstrated this approach can facilitate near-infrared imaging of mRNA localization in vivo and in ex-vivo tissue sections, which will facilitate mRNA trafficking studies in pre-clinical models. Overall, we demonstrate the ability to study fundamental mechanisms necessary to optimize delivery and therapeutic strategies, in order to design the next generation of novel mRNA therapeutics and vaccines.

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“…A limitation of other delivery techniques is their dependence on active uptake pathways such as endocytosis which can lead to sequestration of the cargo rendering it unavailable to function in the cell. For example, liposome-mediated delivery involves both clathrin- and caveolar-mediated endocytosis [ 16 ] while a contribution of macropinocytosis has also been demonstrated to be involved in protein delivery [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

“…Flow cytometry analysis was carried out at 24 hr. As a positive control, dynasore hydrate was used to block liposome-mediated delivery, which functions via both clathrin- and caveolar-mediated endocytosis [ 16 ]. Lipofectamine 2000 (Invitrogen) was used according to manufacturer’s instructions to deliver EGFP mRNA to A549 cells.…”

Section: Methodsmentioning

confidence: 99%

Vector-free intracellular delivery by reversible permeabilization

et al. 2017

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Despite advances in intracellular delivery technologies, efficient methods are still required that are vector-free, can address a wide range of cargo types and can be applied to cells that are difficult to transfect whilst maintaining cell viability. We have developed a novel vector-free method that uses reversible permeabilization to achieve rapid intracellular delivery of cargos with varying composition, properties and size. A permeabilizing delivery solution was developed that contains a low level of ethanol as the permeabilizing agent. Reversal of cell permeabilization is achieved by temporally and volumetrically controlling the contact of the target cells with this solution. Cells are seeded in conventional multi-well plates. Following removal of the supernatant, the cargo is mixed with the delivery solution and applied directly to the cells using an atomizer. After a short incubation period, permeabilization is halted by incubating the cells in a phosphate buffer saline solution that dilutes the ethanol and is non-toxic to the permeabilized cells. Normal culture medium is then added. The procedure lasts less than 5 min. With this method, proteins, mRNA, plasmid DNA and other molecules have been delivered to a variety of cell types, including primary cells, with low toxicity and cargo functionality has been confirmed in proof-of-principle studies. Co-delivery of different cargo types has also been demonstrated. Importantly, delivery occurs by diffusion directly into the cytoplasm in an endocytic-independent manner. Unlike some other vector-free methods, adherent cells are addressed in situ without the need for detachment from their substratum. The method has also been adapted to address suspension cells. This delivery method is gentle yet highly reproducible, compatible with high throughput and automated cell-based assays and has the potential to enable a broad range of research, drug discovery and clinical applications.

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Transmembrane routes of cationic liposome-mediated gene delivery using human throat epidermis cancer cells

Cited by 18 publications

References 12 publications

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA–protein correlations at the level of single cells

Vector-free intracellular delivery by reversible permeabilization

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