Transmembrane Protease Serine 2 in Prostate Cancer

Vest, Deborah; Schalken, Jack A.; Muir, Gordon

doi:10.1111/j.1464-410x.2010.09313.x

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…PCA3 was therefore used as a positive control, reflecting PCa- specific expression panel. TMPRSS2 [30] and DLX1 [31], [32] are also potential PCa biomarkers, as disclosed by examining microarray data using the Oncomine database, and their expression was similarly evaluated as positive controls. In addition, we evaluated expression of 6 potential housekeeping genes: 18S, ACTB, HMBS, RPL13A, TBP, and UBB, as well as 2 genes considered as prostate-specific genes: KLK2 and KLK3/PSA.…”

Section: Resultsmentioning

confidence: 99%

Differentially Expressed Androgen-Regulated Genes in Androgen-Sensitive Tissues Reveal Potential Biomarkers of Early Prostate Cancer

et al. 2013

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BackgroundSeveral data favor androgen receptor implication in prostate cancer initiation through the induction of several gene activation programs. The aim of the study is to identify potential biomarkers for early diagnosis of prostate cancer (PCa) among androgen-regulated genes (ARG) and to evaluate comparative expression of these genes in normal prostate and normal prostate-related androgen-sensitive tissues that do not (or rarely) give rise to cancer.MethodsARG were selected in non-neoplastic adult human prostatic epithelial RWPE-1 cells stably expressing an exogenous human androgen receptor, using RNA-microarrays and validation by qRT-PCR. Expression of 48 preselected genes was quantified in tissue samples (seminal vesicles, prostate transitional zones and prostate cancers, benign prostatic hypertrophy obtained from surgical specimens) using TaqMan® low-density arrays. The diagnostic performances of these potential biomarkers were compared to that of genes known to be associated with PCa (i.e. PCA3 and DLX1).Results and DiscussionBy crossing expression studies in 26 matched PCa and normal prostate transitional zone samples, and 35 matched seminal vesicle and PCa samples, 14 genes were identified. Similarly, 9 genes were overexpressed in 15 benign prostatic hypertrophy samples, as compared to PCa samples. Overall, we selected 8 genes of interest to evaluate their diagnostic performances in comparison with that of PCA3 and DLX1. Among them, 3 genes: CRYAB, KCNMA1 and SDPR, were overexpressed in all 3 reference non-cancerous tissues. The areas under ROC curves of these genes reached those of PCA3 (0.91) and DLX1 (0.94).ConclusionsWe identified ARG with reduced expression in PCa and with significant diagnostic values for discriminating between cancerous and non-cancerous prostatic tissues, similar that of PCA3. Given their expression pattern, they could be considered as potentially protective against prostate cancer. Moreover, they could be complementary to known genes overexpressed in PCa and included along with them in multiplex diagnostic tools.

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Section: Resultsmentioning

confidence: 99%