Objective. To perform a cross-cultural adaptation into Italian and to analyse reliability and validity of the Transsexual Voice Questionnaire for male-to-female transsexuals (I-TVQ MtF). Study Design. Cross-sectional non-randomized survey study. Methods. For item-generation, a cross-cultural adaptation and translation process was performed following standard guidelines. Transgender women were consecutively recruited and asked to fill out the I-TVQ MtF and a form on social, demographic and transition-related variables. Firstly, data collected from participants were used to perform confirmatory factor analysis (CFA), and to evaluate internal consistency and test-retest reliability Subsequently, convergent validity was evaluated comparing I-TVQ MtF total scores with the two extra items addressing self-perception (SPVF) and aspiration (AVF) of voice femininity. To further evaluate convergent validity, scores of the Italian version of the Voice Handicap Index (I-VHI) were considered for comparisons. A correlation analysis was performed to verify potential association between I-TVQ MtF scores and social, demographic and transition-related variables. Results. CFA demonstrated that a two-factor model fits data better than the unidimensional one. Both internal consistency and test retest reliability of the I-TVQ MtF were satisfactory. Negative correlations were highlighted between I-TVQ MtF scores on one side and SPVF and AVF on the other. Positive correlations between I-TVQ MtF and I-VHI scores were also found. Finally, negative correlations were demonstrated between I-TVQ MtF scores and time spent living in the female role. Conclusion. The I-TVQ MtF appears to be a reliable and valid instrument for the assessment of voice-related quality of life in transgender women.