TRANSLUCENT GREEN, an ERF Family Transcription Factor, Controls Water Balance in Arabidopsis by Activating the Expression of Aquaporin Genes

Zhu, Danling; Wu, Zhe; Cao, Guangyu; Li, Jigang; Jia, Wei; Tsuge, Tomohiko; Gu, Hongya; Aoyama, Takashi; Liu, Qu

doi:10.1093/mp/sst152

Cited by 56 publications

(52 citation statements)

References 73 publications

(76 reference statements)

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“…Transcription factors regulate the expression of the genes involved in different processes (Chen et al, 2006;Qu and Zhu, 2006;Guo et al, 2009;Wu et al, 2011;Zhang et al, 2011Zhang et al, , 2012Zhu et al, 2013), and the tail module subunits in the Mediator complex are known to interact with specific transcription factors regulating transcription (Casamassimi and Napoli, 2007). However, we did not find any transcription factor that interacted with YID1/MED16 in a yeasttwo-hybrid assay using the Arabidopsis transcription factor collection (Ou et al, 2011).…”

Section: Yid1/med16 Interacts With Med25contrasting

confidence: 58%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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These authors contributed equally to this work. SUMMARYIron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T-DNA insertion mutant, yellow and sensitive to iron-deficiency 1 (yid1), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID1 encodes the Arabidopsis Mediator complex subunit MED16. We demonstrated that YID1/MED16 interacted with another subunit, MED25. MED25 played an important role in regulation of iron homeostasis by interacting with EIN3 and EIL1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT, IRT1 and FRO2 were reduced in the yid1 and med25 mutants under iron-deficient conditions. The finding that YID1/MED16 and MED25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.

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Section: Yid1/med16 Interacts With Med25contrasting

confidence: 58%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

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“…Next, the interactions between RZ-1B/RZ-1C and SR proteins were confirmed in wild tobacco (Nicotiana benthamiana) leaf epidermal cells, with U1-70K, an empty vector, and TRANSLUCENT GREEN (TG), an unrelated nuclear protein (Zhu et al, 2014), used as negative controls. The recombined YFP fluorescence signal was detected in the nucleus for all interaction pairs ( Figure 5A).…”

Section: Rz-1b/1c Proteins Interact With Sr Proteins Through the C Tementioning

confidence: 99%

“…For subcellular localization ( Figure 4B), fragments of RZ-1C, including the RRM, RRM plus zinc finger, and C terminus plus zinc finger, were cloned to generate transgenic lines. For the flowering time assay, the full-length RZ-1C gene was cloned into pJim19-HA-FLAG (Zhu et al, 2014) to generate transgenic plants in which HA-FLAG-RZ-1C was driven by the CaMV 35S promoter.…”

Section: Overexpression Of Rz-1c and Its Domainsmentioning

confidence: 99%

RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

Zhu

Lin

et al. 2015

Plant Cell

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Nuclear-localized RNA binding proteins are involved in various aspects of RNA metabolism, which in turn modulates gene expression. However, the functions of nuclear-localized RNA binding proteins in plants are poorly understood. Here, we report the functions of two proteins containing RNA recognition motifs, RZ-1B and RZ-1C, in Arabidopsis thaliana. RZ-1B and RZ-1C were localized to nuclear speckles and interacted with a spectrum of serine/arginine-rich (SR) proteins through their C termini. RZ-1C preferentially bound to purine-rich RNA sequences in vitro through its N-terminal RNA recognition motif. Disrupting the RNA binding activity of RZ-1C with SR proteins through overexpression of the C terminus of RZ-1C conferred defective phenotypes similar to those observed in rz-1b rz-1c double mutants, including delayed seed germination, reduced stature, and serrated leaves. Loss of function of RZ-1B and RZ-1C was accompanied by defective splicing of many genes and global perturbation of gene expression. In addition, we found that RZ-1C directly targeted FLOWERING LOCUS C (FLC), promoting efficient splicing of FLC introns and likely also repressing FLC transcription. Our findings highlight the critical role of RZ-1B/1C in regulating RNA splicing, gene expression, and many key aspects of plant development via interaction with proteins including SR proteins.

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“…TG is an AP2/EREBP family transcription factor that directly binds to the promoters of three aquaporin genes (AtTIP1;1, AtTIP2;3 and AtPIP2;2). TG-mediated control of water balance was also evident as the A. thaliana overexpressor lines showed considerable increase in drought tolerance (Zhu et al, 2014). Thus, the overall regulation of aquaporin functions is controlled at the level of transcriptional, post-transcriptional, cytosolic environment and external environmental conditions.…”

Section: Brief Information About Different Modes Of Regulation For Aqmentioning

confidence: 96%

Multifaceted roles of aquaporins as molecular conduits in plant responses to abiotic stresses

Srivastava¹,

Suprasanna²,

Nguyen³

et al. 2014

Critical Reviews in Biotechnology

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Abiotic stress has become a challenge to food security due to occurrences of climate change and environmental degradation. Plants initiate molecular, cellular and physiological changes to respond and adapt to various types of abiotic stress. Understanding of plant response mechanisms will aid in strategies aimed at improving stress tolerance in crop plants. One of the most common and early symptoms associated with these stresses is the disturbance in plant-water homeostasis, which is regulated by a group of proteins called "aquaporins". Aquaporins constitute a small family of proteins which are classified further on the basis of their localization, such as plasma membrane intrinsic proteins, tonoplast intrinsic proteins, nodulin26-like intrinsic proteins (initially identified in symbiosomes of legumes but also found in the plasma membrane and endoplasmic reticulum), small basic intrinsic proteins localized in ER (endoplasmic reticulum) and X intrinsic proteins present in plasma membrane. Apart from water, aquaporins are also known to transport CO2, H2O2, urea, ammonia, silicic acid, arsenite and wide range of small uncharged solutes. Besides, aquaporins also function to modulate abiotic stress-induced signaling. Such kind of versatile functions has made aquaporins a suitable candidate for development of transgenic plants with increased tolerance toward different abiotic stress. Toward this endeavor, the present review describes the versatile functions of aquaporins in water uptake, nutrient balancing, long-distance signal transfer, nutrient/heavy metal acquisition and seed development. Various functional genomic studies showing the potential of specific aquaporin isoforms for enhancing plant abiotic stress tolerance are summarized and future research directions are given to design stress-tolerant crops.

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TRANSLUCENT GREEN, an ERF Family Transcription Factor, Controls Water Balance in Arabidopsis by Activating the Expression of Aquaporin Genes

Cited by 56 publications

References 73 publications

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

Multifaceted roles of aquaporins as molecular conduits in plant responses to abiotic stresses

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