Translocation Pathway of the Intratracheally Instilled Ultrafine Particles from the Lung into the Blood Circulation in the Mouse

Shimada, Akinori; Kawamura, Naoshi; Okajima, Mina; Kaewamatawong, Theerayuth; Inoue, Hiromi; Morita, Takehito

doi:10.1080/01926230601080502

Cited by 167 publications

(104 citation statements)

References 35 publications

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“…The following nanoparticles have been reported to be translocated at the ABB in association with endocytosis in vivo: gold particles (Furuyama et al 2009;Takenaka et al 2006); carbon particles (Shimada et al 2006); and C60 fullerene particles (Naota et al 2009). In in vitro experiments, internalization of 500 nm-sized latex breads (Rejman et al 2004) and 60 nmsized polystyrene particles (Xia et al 2008) by caveolaemediated endocytosis has been reported.…”

Section: Discussionmentioning

confidence: 99%

Caveolae-mediated Endocytosis of Intratracheally Instilled Gold Colloid Nanoparticles at the Air–Blood Barrier in Mice

et al. 2012

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Endocytosis is the primary mechanism by which nanoparticles are translocated over the alveolar epithelium. The purpose of this study was to elucidate the association between endocytosis and the translocation of nanoparticles at the air-blood barrier (ABB). Gold colloid particles (diameter, 20 nm) were intratracheally instilled into male ICR mice. Fifteen minutes after instillation, localized accumulation of agglomerated gold particles was observed in the cytoplasm of macrophages, on the surface of alveolar epithelial cells (AECs), and in alveoli. Electron microscopy revealed particles in the vesicles of macrophages, on the surface of AECs, and in caveolae-like vesicles in type 1 AECs. Immunohistochemistry demonstrated positive immunolabeling for caveolin-1 in the ABB of untreated lungs as well as lungs treated with gold particles. Double immunofluorescence and immunoelectron microscopy revealed the presence of caveolin-1 in AECs in the untreated lungs. These results suggest that instilled gold colloid particles are internalized into the alveolar epithelium at the ABB by caveolae-mediated endocytosis, which is regarded as a physiological function of AECs.

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Section: Discussionmentioning

confidence: 99%

Caveolae-mediated Endocytosis of Intratracheally Instilled Gold Colloid Nanoparticles at the Air–Blood Barrier in Mice

et al. 2012

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“…However, at the same time, NP can directly interact with circulatory cells such as erythrocytes, leukocytes or platelets and potentially induce undesirable toxicity [4,5,6,7]. Therefore, investigation of the direct impact of SiNP on erythrocytes is needed, and will help to understand the possible pathophysiological effects of those particles on erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Amorphous Silica Nanoparticles with Erythrocytesin Vitro: Role of Oxidative Stress

Nemmar¹,

Beegam²,

Yuvaraju³

et al. 2014

Cell Physiol Biochem

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Background/Aims: The use of engineered nanomaterials in the form of nanoparticles (NP) for various biomedical applications, as well as in consumer products, has raised concerns about their safety for human health. These NP are intended to be administered directly into the circulation following intravenous injection, or they may reach the circulation following other routes of administration such as oral or inhalation, and interact with circulating cells such as erythrocytes. However, little is known about the interaction of amorphous SiNP with erythrocytes. Methods: We studied the interaction of amorphous silica nanoparticles (SiNP) at various concentrations (1, 5, 25 and 125µg/ml) with mouse erythrocytes in vitro. Results: Incubation of erythrocytes with SiNP caused a dose-dependent hemolytic effect. Likewise, the activity of lactate dehydrogenase was dose-dependently increased by SiNP. Transmission electron microscopy analysis revealed that SiNP are taken up by erythrocytes. Lipid erythrocyte susceptibility to in vitro peroxidation measured by malondialdehyde showed a significant and dose-dependent increase in erythrocytes. SiNP also enhanced the antioxidant activities of superoxide dismutase (SOD), catalase and reduced glutathione (GSH). Moreover, SiNP increased caspase 3, triggered annexin V-binding and caused a dose-dependent increase of cytosolic calcium concentration. Conclusion: It can be concluded that SiNP cause a dose-dependent hemolytic activity and are taken up by the erythrocytes. We also found that SiNP induce the occurrence of oxidative activity, apoptosis and increase cytosolic Ca²⁺, which may explain their haemolytic activity. Our in vitro data suggest that SiNP may, plausibly, lead to anemia and circulatory disorders in vivo.

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“…Values of SNARK mRNA expressions were normalized to -actin mRNA expression; C -control; n = 3; * -P < 0.05 [15]. Shimada et al [49] demonstrated that the intratracheally instilled ultrafine nanoparticles are able to translocate from the mouse lung into systemic circulation. Precise mechanisms of the anatomical translocation (crossing the air-blood barrier) of inhaled nanoparticles at the alveolar wall are not fully understood.…”

Section: Snark Kinase As a Stress Sensor And Sensitive Marker Of Silvmentioning

confidence: 99%

SNF1/AMP-Activated Protein Kinases: Genes, Expression and Biological Role

Minchenko¹,

Minchenko²

2012

Protein Kinases

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Translocation Pathway of the Intratracheally Instilled Ultrafine Particles from the Lung into the Blood Circulation in the Mouse

Cited by 167 publications

References 35 publications

Caveolae-mediated Endocytosis of Intratracheally Instilled Gold Colloid Nanoparticles at the Air–Blood Barrier in Mice

Caveolae-mediated Endocytosis of Intratracheally Instilled Gold Colloid Nanoparticles at the Air–Blood Barrier in Mice

Interaction of Amorphous Silica Nanoparticles with Erythrocytesin Vitro: Role of Oxidative Stress

SNF1/AMP-Activated Protein Kinases: Genes, Expression and Biological Role

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