Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos

El, Furno; Busseau, Isabelle; Lorenzi, Claudio; Saghira, Cima; Züchner, Stephan; Maiorano, Domenico

doi:10.1101/2020.11.28.401471

Cited by 2 publications

(2 citation statements)

References 77 publications

(136 reference statements)

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“…It has been observed that during the very early stage of Xenopus embryonic development, the HDR activity of Rad18 is coupled to its TLS activity, through PCNA mUb , thereby reducing the mutational load introduced. This mechanism adds up to the MMR activity that corrects mismatches introduced by TLS [ 73 ]. Taking into consideration this observation, it is possible that, in vivo, the mutagenesis rate of TLS Pols may be underestimated due to the concerted action of Rad18 repair activity.…”

Section: The Crucial Role Of Tls To Tolerate Structural Impediments During Dna Replicationmentioning

confidence: 99%

Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress

Maiorano

Etri

Franchet

et al. 2021

IJMS

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DNA can experience “replication stress”, an important source of genome instability, induced by various external or endogenous impediments that slow down or stall DNA synthesis. While genome instability is largely documented to favor both tumor formation and heterogeneity, as well as drug resistance, conversely, excessive instability appears to suppress tumorigenesis and is associated with improved prognosis. These findings support the view that karyotypic diversity, necessary to adapt to selective pressures, may be limited in tumors so as to reduce the risk of excessive instability. This review aims to highlight the contribution of specialized DNA polymerases in limiting extreme genetic instability by allowing DNA replication to occur even in the presence of DNA damage, to either avoid broken forks or favor their repair after collapse. These mechanisms and their key regulators Rad18 and Polθ not only offer diversity and evolutionary advantage by increasing mutagenic events, but also provide cancer cells with a way to escape anti-cancer therapies that target replication forks.

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Section: The Crucial Role Of Tls To Tolerate Structural Impediments During Dna Replicationmentioning

confidence: 99%

Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress

Maiorano

Etri

Franchet

et al. 2021

IJMS

Self Cite

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show abstract

“…Additionally, cold sock causes the opening of interchain disulfide bridges in protamines that may interfere with sperm DNA [ 9 ]. DNA integrity is necessary for embryo development [ 10 ]. Higher sperm SDF resulted in lower fertilization rates [ 11 ], lower pregnancy rate [ 12 ], and finally, lower pregnancy rate [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm

Mustofa

Susilowati

Wurlina

et al. 2021

Heliyon

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The study aimed to determine the addition of green tea extract (GTE) in extender on the quality and DNA mutation of post-thawed Kacang buck sperm. The sperm DNA mutation was observed on nicotinamide adenine dinucleotide hydride (NADH) dehydrogenase 1 (ND1) of mitochondrial Deoxyribonucleic Acid (mtDNA). A pool of 12 Kacang buck ejaculates was diluted in skim milk-egg yolk extender contained 0, 0.05, 0.10, and 0.15 mg of GTE/100 mL for T0, T1, T2, and T3 group, respectively. Each of the aliquot groups was packaged in 0.25 mL French mini straw contained 60 million alive sperm and froze according to the protocol. The ND1 mtDNA amplification of samples was carried out Polymerase Chain Reaction machine, followed by DNA sequencing using the Sanger method. Meanwhile, the phylogenetic tree was constructed using the neighbor-joining (NJ) method with MEGA 7.0 software. The results showed that the T2 group maintained the highest quality for Kacang buck post-thawed semen. There was the highest percentages of sperms viability, motility, intact plasma membrane (IPM), the lowest of malondialdehyde (MDA) concentration, sperm DNA fragmentation (SDF), the total and types of ND1 mtDNA mutation frequency. The phylogenetic tree analysis revealed that the clade of the T2 group was most closely related to the sequence reference. However, there was no correlation between the semen quality parameters (sperm viability, motility, IPM, MDA concentration, and SDF) with ND1 mtDNA mutation of post-thawed Kacang buck semen. It could be concluded that GTE was useful as an antioxidant for Kacang buck semen extender for frozen sperm.

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Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos

Cited by 2 publications

References 77 publications

Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress

Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress

Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm

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