Translationally controlled tumor protein (TCTP) in the human prostate and prostate cancer cells: Expression, distribution, and calcium binding activity

Arcuri, Felice; Papa, Stefania; Carducci, A; Romagnoli, Roberta; Liberatori, Sabrina; Riparbelli, Maria Giovanna; Sanchez, Jean‐Charles; Tosi, Piero; Vecchio, Maria Teresa Del

doi:10.1002/pros.20054

Cited by 99 publications

(94 citation statements)

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“…Later, it was shown that TCTP levels are regulated in response to perturbations of intracellular Ca 2+ -homeostasis (Xu et al 1999). Although TCTP does not have a canonical Ca 2+ -binding site , its Ca 2+ -binding activity has been confirmed by several laboratories using a range of methods (Feng et al 2007a;Graidist et al 2007;Kim et al 2000;Lucas et al 2014;Arcuri et al 2004Arcuri et al , 2005Sanchez et al 1997). However, the different approaches used to map the Ca 2+ -binding site at the TCTP protein yielded different results (Feng et al 2007a;Graidist et al 2007;Kim et al 2000).…”

Section: Involvement In Cellular Stress Responsesmentioning

confidence: 99%

“…The importance of such a Ca 2+ -buffer-like function for TCTP was described for trophoblast cells of the placenta, where it is involved in Ca 2+ -handling and in its provision for the foetal blood circulation (Arcuri et al 2005). Similarly, in human prostate epithelial cells, TCTP is the Ca 2+ -binding protein with the highest expression levels (Arcuri et al 2004). Another important player in maintaining intracellular ion homeostasis that is influenced by TCTP is the membrane-bound Na + ,K + -ATPase.…”

Section: Involvement In Cellular Stress Responsesmentioning

confidence: 99%

“…The first reports on the role for TCTP in the mammalian reproductive system appeared about 15 years ago. Specifically, the developmental expression of TCTP in the rat and human testes were studied by the group of Sanchez and Hochstrasser in Geneva (Guillaume et al 2001), and the importance of TCTP as a Ca 2+ -binding protein in the prostate (Arcuri et al 2004) and the placenta (Arcuri et al 2005) were demonstrated by Arcuri et al A more recent study showed that TCTP is essential for the implantation of embryos in the uterus of mice ). An important role for TCTP in egg production in nematodes, such as C. elegans, was demonstrated by TCTP knock-down, which severely reduced the number of eggs produced by these worms (Meyvis et al 2009), consistent with the role of TCTP for meiotic maturation demonstrated in mouse oocytes (Jeon et al 2016).…”

Section: Roles For Tctp In Early Developmentmentioning

confidence: 99%

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The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

Bommer

2017

Results and Problems in Cell Differentiation

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The Translational Controlled Tumour Protein TCTP (gene symbol TPT1, also called P21, P23, Q23, fortilin or histamine-releasing factor, HRF) is a highly conserved protein present in essentially all eukaryotic organisms and involved in many fundamental cell biological and disease processes. It was first discovered about 35 years ago, and it took an extended period of time for its multiple functions to be revealed, and even today we do not yet fully understand all the details. Having witnessed most of this history, in this chapter, I give a brief overview and review the current knowledge on the structure, biological functions, disease involvements and cellular regulation of this protein. TCTP is able to interact with a large number of other proteins and is therefore involved in many core cell biological processes, predominantly in the response to cellular stresses, such as oxidative stress, heat shock, genotoxic stress, imbalance of ion metabolism as well as other conditions. Mechanistically, TCTP acts as an anti-apoptotic protein, and it is involved in DNA-damage repair and in cellular autophagy. Thus, broadly speaking, TCTP can be considered a cytoprotective protein. In addition, TCTP facilitates cell division through stabilising the mitotic spindle and cell growth through modulating growth signalling pathways and through its interaction with the proteosynthetic machinery of the cell. Due to its activities, both as an anti-apoptotic protein and in promoting cell growth and division, TCTP is also essential in the early development of both animals and plants. Apart from its involvement in various biological processes at the cellular level, TCTP can also act as an extracellular protein and as such has been involved in modulating whole-body defence processes, namely in the mammalian immune system. Extracellular TCTP, typically in its dimerised form, is able to induce the release of cytokines and other signalling molecules from various types of immune cells. There are also several examples, where TCTP was shown to be involved in antiviral/antibacterial defence in lower animals. In plants, the protein appears to have a protective effect against phytotoxic stresses, such as flooding, draught, too high or low temperature, salt stress or exposure to heavy metals. The finding for the latter stress condition is corroborated by earlier reports that TCTP levels are considerably up-regulated upon exposure of earthworms to high levels of heavy metals. Given the involvement of TCTP in many biological processes aimed at maintaining cellular or whole-body homeostasis, it is not surprising that dysregulation of TCTP levels may promote a range of disease processes, foremost cancer. Indeed a large body of evidence now supports a role of TCTP in at least the most predominant types of human cancers. Typically, this can be ascribed to both the anti-apoptotic activity of the protein and to its function in promoting cell growth and division. However, TCTP also appears to be involved in the later stages of cancer progression, such ...

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Section: Involvement In Cellular Stress Responsesmentioning

confidence: 99%

Section: Involvement In Cellular Stress Responsesmentioning

confidence: 99%

Section: Roles For Tctp In Early Developmentmentioning

confidence: 99%

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The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

Bommer

2017

Results and Problems in Cell Differentiation

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“…TCTP overexpression has previously been associated with numerous malignancies. [72][73][74] Immunofluorescent histologic analysis of cells exposed to LB102 exhibited abnormal mitotic figures and distorted microtubules, characteristics of mitotic catastrophe. These in vitro experiments were replicated in U373 GBM cell line with a p53 gain-of-function mutation.…”

Section: Lb100: a Small Molecule Inhibitor Of Pp2amentioning

confidence: 99%

LB100, a small molecule inhibitor of PP2A with potent chemo- and radio-sensitizing potential

Zhang

et al. 2015

Cancer Biology & Therapy

103

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“…It is reported as multifunctional to participate in numerous cellular processes including cell growth, survival, development, protein synthesis, immune response, tumor reversion, transcription regulation, and induction of pluripotent stem cells or apoptosis (Bommer and Thiele 2004). Comparing to normal tissue, the level of TCTP is increased in many human cancer tissues, such as breast, colorectal, prostate and lung cancer (Arcuri et al 2004;Deng et al 2006;Kim et al 2008;Slaby et al 2009). Therefore, in recent years, more and more attention is paid to the involvement of TCTP on tumor reversion because of its critical role in tumorigenesis (Miao et al 2013;Nagano-Ito and Ichikawa 2012).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomics analysis of global cellular stress responses to hydroxyurea-induced DNA damage in HeLa cells

et al. 2014

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Both environmental agents and spontaneous cellular events cause serious DNA damage, threatening the integrity of the genome. In response to replication stress or genotoxic agents triggered DNA damage, degradation of p12 subunit of DNA polymerase delta (Pol d) results in an inter-conversion between heterotetramer (Pol d4) and heterotrimer (Pol d3) forms and plays a significant role in DNA damage response in eukaryotic cells. In this work, we used mass spectrometry-based proteomic approach to identify those cellular stress response protein changes corresponding to the degradation of p12 in DNAdamaged HeLa cells by the treatment with hydroxyurea (HU). A total of 736 ± 13 proteins in non-treated control group and 741 ± 19 protein spots in HUtreated cells were detected, of which 34 proteins (17 up-regulated and 17 down-regulated) exhibited significantly altered protein expression levels. Their physiological roles are mainly associated with cellular components, molecular functions, and biological processes by gene ontology analysis, among which 21 proteins were mapped to KEGG pathways. They are involved in 5 primary pathways with the subsets involving 16 secondary pathways by further KEGG analysis. More interestingly, the up-regulation of translationally controlled tumor protein was further identified to be associated with p12 degradation by Western blot analysis. Our works may enlarge and broaden our view for deeply understanding how global cellular stress responds to DNA damage, which could contribute to the etiology of human cancer or other diseases that can result from loss of genomic stability.

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Translationally controlled tumor protein (TCTP) in the human prostate and prostate cancer cells: Expression, distribution, and calcium binding activity

Abstract: The results of the present study demonstrate, for the first time, the expression of TCTP in the human prostate and in prostate cancer cells, and suggest the involvement of the protein in key-processes such as apoptosis, cellular differentiation, and in the control of sperm functions.

Cited by 99 publications

References 49 publications

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

LB100, a small molecule inhibitor of PP2A with potent chemo- and radio-sensitizing potential

Comparative proteomics analysis of global cellular stress responses to hydroxyurea-induced DNA damage in HeLa cells

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