Translational impact of novel widely pharmacological characterized mofezolac-derived COX-1 inhibitors combined with bortezomib on human multiple myeloma cell lines viability

Pati, Maria Laura; Vitale, Paola; Ferorelli, Savina; Iaselli, Mariaclara; Miciaccia, Morena; Boccarelli, Angelina; Mauro, Giuseppe Davide Di; Fortuna, Cosimo G.; Domingos, Thaisa Francielle Souza; Silva, Luiz Cláudio Rodrigues Pereira da; Pádula, Marcelo de; Cabral, Lúcio Mendes; Sathler, P.C; Vacca, Angelo; Scilimati, Antonio; Perrone, Maria Grazia

doi:10.1016/j.ejmech.2018.12.029

Cited by 13 publications

(6 citation statements)

References 49 publications

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“…The apoptotic rate of the cells treated with bortezomib was significantly higher than that of the untreated control group (P<0.05), which also showed that bortezomib has the effect of promoting myeloma cell apoptosis. These results are consistent with previous studies ( 26 , 27 ). Finally, the expression levels of Wnt/β-catenin signaling pathway-related proteins β-catenin and c-Myc were detected in each group by two methods.…”

Section: Discussionsupporting

confidence: 94%

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

2020

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Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β-catenin signaling pathway was investigated. Myeloma cells RPMI-8226 treated with different concentrations of bortezomib were used as experimental groups, and the untreated cells were used as the control group. The proliferation and apoptosis in all groups of cells were detected, as well as the expression levels of Wnt/β-catenin signaling pathway-related proteins, β-catenin and c-Myc. The results revealed that bortezomib could inhibit the proliferation of myeloma cells. The apoptotic rates of RPMI-8226 cells in the groups treated with 20, 50 and 80 nmol/l of bortezomib were 12.08±0.61, 35.97±3.11 and 57.22±5.47%, respectively, which were significantly higher than that in the control group (8.28±0.39%) (P<0.05). The expression levels of β-catenin and c-Myc in the experimental groups were significantly lower than those in the control group (P<0.05). Bortezomib can reduce the expression level of Wnt/β-catenin signaling pathway-related proteins, β-catenin and c-Myc, and may inhibit cell proliferation and accelerate apoptosis by activating the Wnt/β-catenin signaling pathway.

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Section: Discussionsupporting

confidence: 94%

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

2020

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“…COX catalyzes the first step in the biosynthesis of prostaglandins (PG), prostacyclin and thromboxane starting from free arachidonic acid (AA) [ 43 ]. Among prostaglandins, PGE 2 is involved in inflammation, angiogenesis and in promoting the growth of several solid tumors, such as breast, ovarian, head and neck cancer, renal cell carcinoma and hematological cancers [ 44 , 45 , 46 ]. COX-1 and COX-2 are of great interest because they are targets of non–steroidal anti–inflammatory drugs (NSAIDs), which, when binding to the active site of COX, prevent the AA from reaching the catalytic pocket and, thus, the biosynthesis of prostaglandins.…”

Section: Discussionmentioning

confidence: 99%

Olive Mill Wastewater Fermented with Microbial Pools as a New Potential Functional Beverage

et al. 2023

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Olive mill wastewater (OMWW) represents a by–product but also a source of biologically active compounds, and their recycling is a relevant strategy to recover income and to reduce environmental impact. The objective of the present study was to obtain a new functional beverage with a health–promoting effect starting from OMWW. Fresh OMWW were pre–treated through filtration and/or microfiltration and subjected to fermentation using strains belonging to Lactiplantibacillus plantarum, Candida boidinii and Wickerhamomyces anomalus. During fermentation, phenolic content and hydroxytyrosol were monitored. Moreover, the biological assay of microfiltered fermented OMWW was detected versus tumor cell lines and as anti-inflammatory activity. The results showed that in microfiltered OMWW, fermentation was successfully conducted, with the lowest pH values reached after 21 days. In addition, in all fermented samples, an increase in phenol and organic acid contents was detected. Particularly, in samples fermented with L. plantarum and C. boidinii in single and combined cultures, the concentration of hydroxytyrosol reached values of 925.6, 902.5 and 903.5 mg/L, respectively. Moreover, biological assays highlighted that fermentation determines an increase in the antioxidant and anti–inflammatory activity of OMWW. Lastly, an increment in the active permeability on Caco-2 cell line was also revealed. In conclusion, results of the present study confirmed that the process applied here represents an effective strategy to achieve a new functional beverage.

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“…The western blotting analysis was conducted by using the methods reported in Perrone et al with minor modifications [38]. Briefly, all cells were washed twice with 10 mL phosphate-buffered saline (PBS), scraped in 1 mL PBS and centrifuged for 10 min at 1500 rpm, 4 • C. Proteins were extracted from cells by homogenization in cold RIPA buffer (Sigma-Aldrich) containing 1X protease inhibitor cocktail and centrifuged at 14,000 rpm for 10 min at 4 • C. The supernatant was recovered, and the protein concentration was measured using a DC Protein Assay Reagent Kit (BIO-RAD, Hercules, CA, USA).…”

Section: Western Blottingmentioning

confidence: 99%

Intelligent Microarray Data Analysis through Non-negative Matrix Factorization to Study Human Multiple Myeloma Cell Lines

et al. 2019

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Microarray data are a kind of numerical non-negative data used to collect gene expression profiles. Since the number of genes in DNA is huge, they are usually high dimensional, therefore they require dimensionality reduction and clustering techniques to extract useful information. In this paper we use NMF, non-negative matrix factorization, to analyze microarray data, and also develop “intelligent” results visualization with the aim to facilitate the analysis of the domain experts. For this purpose, a case study based on the analysis of the gene expression profiles (GEPs), representative of the human multiple myeloma diseases, was investigated in 40 human myeloma cell lines (HMCLs). The aim of the experiments was to study the genes involved in arachidonic acid metabolism in order to detect gene patterns that possibly could be connected to the different gene expression profiles of multiple myeloma. NMF results have been verified by western blotting analysis in six HMCLs of proteins expressed by some of the most abundantly expressed genes. The experiments showed the effectiveness of NMF in intelligently analyzing microarray data.

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Translational impact of novel widely pharmacological characterized mofezolac-derived COX-1 inhibitors combined with bortezomib on human multiple myeloma cell lines viability

Cited by 13 publications

References 49 publications

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

Olive Mill Wastewater Fermented with Microbial Pools as a New Potential Functional Beverage

Intelligent Microarray Data Analysis through Non-negative Matrix Factorization to Study Human Multiple Myeloma Cell Lines

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