Translational Control in Oocyte Development

Richter, Joel D.; Lasko, Paul

doi:10.1101/cshperspect.a002758

Cited by 110 publications

(91 citation statements)

References 138 publications

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“…These observations are compatible with a model in which the catalytic activity of PARP12 serves as a molecular switch enabling, possibly through a conformational change, the efficient translational blockade of numerous cellular mRNAs. This conclusion is also supported by the high abundance of PARP12 mRNA in oocytes (a site of intense translational control (50).…”

Section: Discussionsupporting

confidence: 61%

PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation

Welsby

Hutin

Gueydan

et al. 2014

Journal of Biological Chemistry

101

115

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Background:The individual role of members of the poly(ADP-ribose) polymerase family is unclear. Results: PARP12 displays a dual subcellular localization and effector function, controlling both protein translation and NF-B signaling. Conclusion: PARP12 mediates two important effector mechanisms linked to the establishment of an anti-viral state. Significance: ADP-ribosylation may play an important role in the innate control of microbial infections.

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Section: Discussionsupporting

confidence: 61%

PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation

Welsby

Hutin

Gueydan

et al. 2014

Journal of Biological Chemistry

101

115

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“…In contrast, the Orb2 dataset showed overall enrichment for G-containing motifs. A more detailed comparison of individual motif types shows that the Orb dataset is enriched in CPE variants containing A in the fifth position [TTTT(A) [1][2][3] T], whereas the TTTTGT and TTTTAAAT motifs are the most enriched in the Orb2 dataset (Fig. 3C); this difference could explain the difficulty in resolving the fifth nucleotide in de novo analysis.…”

Section: Resultsmentioning

confidence: 99%

RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2

Stepien

Oppitz

Gerlach

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Localized protein translation is critical in many biological contexts, particularly in highly polarized cells, such as neurons, to regulate gene expression in a spatiotemporal manner. The cytoplasmic polyadenylation element-binding (CPEB) family of RNA-binding proteins has emerged as a key regulator of mRNA transport and local translation required for early embryonic development, synaptic plasticity, and long-term memory (LTM). Drosophila Orb and Orb2 are single members of the CPEB1 and CPEB2 subfamilies of the CPEB proteins, respectively. At present, the identity of the mRNA targets they regulate is not fully known, and the binding specificity of the CPEB2 subfamily is a matter of debate. Using transcriptome-wide UV cross-linking and immunoprecipitation, we define the mRNA-binding sites and targets of Drosophila CPEBs. Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3′ UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity. Both proteins use their RNA-recognition motifs but not the Zinc-finger region for RNA binding. A subset of Orb2 targets is translationally regulated in cultured S2 cells and fly head extracts. Moreover, pan-neuronal RNAi knockdown of these targets suggests that a number of these targets are involved in LTM. Our results provide a comprehensive list of mRNA targets of the two CPEB proteins in Drosophila, thus providing insights into local protein synthesis involved in various biological processes, including LTM.CPEB | CLIP | Orb2 | translation | long-term memory

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“…Second and more importantly, they are found in the cytoplasm weeks or even months before nuclear envelope breakdown at the time of oocyte maturation. It is well known that protein synthesis in the oocyte and fertilized egg is highly regulated (Richter 2007;Richter and Lasko 2011) and cytoplasmic sisRNAs could be one of the players in this regulation. Future experiments will focus on the precise cytological localization of sisRNAs and on the proteins with which they are associated.…”

Section: Origin and Biological Role Of Cytoplasmic Sisrnasmentioning

confidence: 99%

Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes

Talhouarne

Gall

2014

RNA

122

141

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We previously demonstrated that the oocyte nucleus (germinal vesicle or GV) of Xenopus tropicalis contains a population of stable RNA molecules derived from the introns of most expressed genes. Here we show that similar stable intronic sequence (sis) RNAs occur in the oocyte cytoplasm. About 9000 cytoplasmic sisRNAs have been identified, all of which are resistant to the exonuclease RNase R. About half have been confirmed as lariat molecules and the rest are presumed to be lariats, whereas nuclear sisRNAs are a mixture of lariat and linear molecules. Cytoplasmic sisRNAs are more abundant on a molar basis than nuclear sisRNAs and are derived from short introns, mostly under 1 kb in length. Both nuclear and cytoplasmic sisRNAs are transmitted intact to the egg at GV breakdown and persist until at least the blastula stage of embryogenesis, when zygotic transcription begins. We compared cytoplasmic sisRNAs derived from orthologous genes of X. tropicalis and X. laevis, and found that the specific introns from which sisRNAs are derived are not conserved. The existence of sisRNAs in the cytoplasm of the oocyte, their transmission to the fertilized egg, and their persistence during early embryogenesis suggest that they might play a regulatory role in mRNA translation.

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Translational Control in Oocyte Development

Cited by 110 publications

References 138 publications

PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation

PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation

RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2

Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes

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