Translational biophotonics with Raman imaging: clinical applications and beyond

Pence, Isaac J.; Evans, Conor L.

doi:10.1039/d1an00954k

Cited by 10 publications

(11 citation statements)

References 117 publications

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“…The structure of 3D SERS significantly enhanced the Raman signal. Rajput et al [235] proposed a method that involved the use of quantitative NPs to enhance sandwich antibody detection and successfully detected castration-resistant PCa in vitro. Figure 15a shows a schematic representation of AR-V7 protein detection in patients with MCPc.…”

Section: Prostate Cancer (Pca)mentioning

confidence: 99%

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Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges

Wang,

Fang,

Wang

et al. 2023

Advanced Science

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Early clinical diagnosis, effective intraoperative guidance, and an accurate prognosis can lead to timely and effective medical treatment. The current conventional clinical methods have several limitations. Therefore, there is a need to develop faster and more reliable clinical detection, treatment, and monitoring methods to enhance their clinical applications. Raman spectroscopy is noninvasive and provides highly specific information about the molecular structure and biochemical composition of analytes in a rapid and accurate manner. It has a wide range of applications in biomedicine, materials, and clinical settings. This review primarily focuses on the application of Raman spectroscopy in clinical medicine. The advantages and limitations of Raman spectroscopy over traditional clinical methods are discussed. In addition, the advantages of combining Raman spectroscopy with machine learning, nanoparticles, and probes are demonstrated, thereby extending its applicability to different clinical phases. Examples of the clinical applications of Raman spectroscopy over the last 3 years are also integrated. Finally, various prospective approaches based on Raman spectroscopy in clinical studies are surveyed, and current challenges are discussed.

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Section: Prostate Cancer (Pca)mentioning

confidence: 99%

“…a) Schematic of the immune analysis process, sandwich immune analysis, and SERS in vitro detection of AR‐V7 protein in serum. [ 235 ] Copyright 2022, American Chemical Society. b) Preparation process of the plasma sensor, schematic of PSA detection, and detected Raman spectrum.…”

Section: Clinical Applications Of Raman Spectroscopymentioning

confidence: 99%

Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges

Wang,

Fang,

Wang

et al. 2023

Advanced Science

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“…Raman excitation can also be selected in optical windows with low background and biological transparency. Raman imaging looks promising for the future of biological imaging [48]. SWCNTs and other novel nanomaterials are widely used in a variety of biological applications, such as drug delivery and imaging, as is well known.…”

Section: Raman Imagingmentioning

confidence: 99%

Cancer Targeting and Diagnosis: Recent Trends with Carbon Nanotubes

Singh

Kumar

2022

Nanomaterials

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Cancer belongs to a category of disorders characterized by uncontrolled cell development with the potential to invade other bodily organs, resulting in an estimated 10 million deaths globally in 2020. With advancements in nanotechnology-based systems, biomedical applications of nanomaterials are attracting increasing interest as prospective vehicles for targeted cancer therapy and enhancing treatment results. In this context, carbon nanotubes (CNTs) have recently garnered a great deal of interest in the field of cancer diagnosis and treatment due to various factors such as biocompatibility, thermodynamic properties, and varied functionalization. In the present review, we will discuss recent advancements regarding CNT contributions to cancer diagnosis and therapy. Various sensing strategies like electrochemical, colorimetric, plasmonic, and immunosensing are discussed in detail. In the next section, therapy techniques like photothermal therapy, photodynamic therapy, drug targeting, gene therapy, and immunotherapy are also explained in-depth. The toxicological aspect of CNTs for biomedical application will also be discussed in order to ensure the safe real-life and clinical use of CNTs.

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“…Raman spectroscopy is a technique that enables direct assessments of chemical bonds through their specific vibrational signatures. , In recent years, many biological uses of Raman methods have been developed, such as the observation of cellular metabolism and physiology, , disease identification, , and cell differentiation. , This is due to its nonintrusive nature and the possibility to distinguish between the spectral signatures of many different biomolecules, which makes it optimal for biomedical applications. , However, the use of simple Raman microspectroscopy to study biological samples can have different disadvantages depending on the measurement condition, such as Raman scattering efficiency, sample autofluorescence, and photodamage. Indeed, to obtain sufficient Raman scattered signals and reduce the acquisition times, short-wavelength lasers are preferentially used.…”

Section: Introductionmentioning

confidence: 99%

Ammonia Toxicity and Associated Protein Oxidation: A Single-Cell Surface Enhanced Raman Spectroscopy Study

Redolfi-Bristol,

Mangiameli,

Yamamoto

et al. 2023

Chem. Res. Toxicol.

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Ammonia (NH 3 ) is a commonly used industrial chemical to which exposure at high concentrations can result in severe skin damage. Moreover, high levels of ammonia in the human body can lead to hyperammonemia conditions and enhanced cancer metabolism. In this work, the toxicity mechanism of NH 3 has been studied against human dermal fibroblast (HDF) cells using surface-enhanced Raman spectroscopy (SERS). For this purpose, gold nanoparticles of size 50 nm have been prepared and used as probes for Raman signal enhancement, after being internalized inside HDF cells. Following the exposure to ammonia, HDF cells showed a significant variation in the protein ternary structure's signals, demonstrating their denaturation and oxidation process, together with early signs of apoptosis. Meaningful changes were observed especially in the Raman vibrations of sulfurcontaining amino acids (cysteine and methionine) together with aromatic residues. Fluorescence microscopy revealed the formation of reactive oxygen and nitrogen species in cells, which confirmed their stressed condition and to whom the causes of protein degradation can be attributed. These findings can provide new insights into the mechanism of ammonia toxicity and protein oxidation at a single-cell level, demonstrating the high potential of the SERS technique in investigating the cellular response to toxic compounds.

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Translational biophotonics with Raman imaging: clinical applications and beyond

Abstract: The technological developments of high-speed spontaneous and coherent Raman scattering based imaging for translational applications including surgical guidance, histopathology, and pathophysiological monitoring.

Cited by 10 publications

References 117 publications

Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges

Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges

Cancer Targeting and Diagnosis: Recent Trends with Carbon Nanotubes

Ammonia Toxicity and Associated Protein Oxidation: A Single-Cell Surface Enhanced Raman Spectroscopy Study

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