Translational and post-translational regulation of polyamine metabolic enzymes in plants

Jiménez-Bremont, Juan Francisco; Chávez-Martínez, Ana Isabel; Ortega-Amaro, María Azucena; Guerrero-González, María de la Luz; Jasso-Robles, Francisco Ignacio; Maruri-López, Israel; Liu, Jihong; Gill, Sarvajeet Singh; Rodríguez‐Kessler, Margarita

doi:10.1016/j.jbiotec.2021.12.004

Cited by 7 publications

(3 citation statements)

References 133 publications

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“…The strong increase in ADC transcript levels in M. truncatula plants grown under NH 4 + nutrition contrasted with the decrease in ADC activity. This suggests the existence of post-transcriptional regulatory mechanisms as the described regulation of the ADC enzymes by upstream open reading frames ( Jiménez-Bremont et al , 2022 ). However, we cannot exclude that the reaction catalyzed by ADC was affected by the following AIH and NCPAH activities, as both release NH 4 + .…”

Section: Discussionmentioning

confidence: 98%

The importance of the urea cycle and its relationships to polyamine metabolism during ammonium stress in Medicago truncatula

Urra

Buezo

Royo

et al. 2022

Journal of Experimental Botany

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The ornithine-urea cycle (urea cycle) makes a significant contribution to the metabolic responses of lower photosynthetic eukaryotes to episodes of high nitrogen availability. We therefore compared the role of the plant urea cycle and its relationships to polyamine metabolism in ammonium-fed and nitrate-fed Medicago truncatula plants. High ammonium resulted in the accumulation of ammonium and pathway intermediates, particularly glutamine, arginine, ornithine, and putrescine. Arginine decarboxylase activity was decreased in roots, suggesting that the ornithine decarboxylase-dependent production of putrescine was important in situations of ammonium stress. The activity of copper-amine oxidase, which releases ammonium from putrescine, was significantly decreased in both shoots and roots. In addition, physiological concentrations of ammonium inhibited copper-amine oxidase activity in in vitro assays, supporting the conclusion that high ammonium accumulation favors putrescine synthesis. Moreover, putrescine early supplemented to plants avoided ammonium toxicity. The levels of transcripts encoding urea cycle-related proteins were increased and transcripts involved in polyamine catabolism were decreased under high ammonium. We conclude that the urea cycle and associated polyamine metabolism function as important protective mechanisms limiting ammonium toxicity in M. truncatula. These findings demonstrate the relevance of the urea cycle to polyamine metabolism in higher plants.

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Section: Discussionmentioning

confidence: 98%

The importance of the urea cycle and its relationships to polyamine metabolism during ammonium stress in Medicago truncatula

Urra

Buezo

Royo

et al. 2022

Journal of Experimental Botany

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“…It is suggested that these alterations can contribute to the lethal effect of 1 mM SA in roots. Further investigations can clarify how this ROS-NO imbalance influences the late responses of tomato roots not only in growth but also stress conditions [17][18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Short-Term Salicylic Acid Treatment Affects Polyamine Metabolism Causing ROS–NO Imbalance in Tomato Roots

Szepesi

Poór

Bakacsy

2022

Plants

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The phytohormone salicylic acid (SA) can influence the polyamine metabolism in plants. Additionally, polyamines (PAs) can regulate the synthesis of SA, providing an exciting interplay between them not only in plant growth and development but also in biotic or abiotic stress conditions. The effect of SA on polyamine metabolism of leaves is well-studied but the root responses are rarely investigated. In this study, tomato roots were used to investigate the effect of short-term exposition of SA in two different concentrations, a sublethal 0.1 mM and a lethal 1 mM. To explore the involvement of SA in regulating PAs in roots, the degradation of PAs was also determined. As both SA and PAs can induce reactive oxygen species (ROS) and nitric oxide (NO) production, the balance of ROS and NO was analyzed in root tips. The results showed that 0.1 mM SA induced the production of higher PAs, spermidine (Spd), and spermine (Spm), while 1 mM SA decreased the PA contents by activating degrading enzymes. Studying the ROS and NO levels in root tips, the ROS production was induced earlier than NO, consistent with all the investigated zones of roots. This study provides evidence for concentration-dependent rapid effects of SA treatments on polyamine metabolism causing an imbalance of ROS–NO in root tips.

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“…When faced with adversity, plants activate defense mechanisms and generate responses to adapt to their environment. Polyamines (PAs), which are closely related to plant resistance, growth, and development, their levels increase in plants during abiotic stress such as salinity, extreme temperature, paraquat or heavy metals, particularly important for adaptation and resistance to cold stress, serving as crucial metabolites for responding to external stimuli [ 6 , 7 ], and are a class of low-molecular-weight, aliphatic, nitrogen-containing bases with strong bioactivities that are widely present in prokaryotic and eukaryotic organisms, mainly including putrescine (Put), spermidine (Spd), spermine (Spm), thermospermine (Tspm) [ 8 ]. Under physiological pH conditions(7.0 ∼ 7.4.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of polyamine metabolism and ethylene synthesis genes in Chenopodium quinoa Willd. and their responses to low-temperature stress

Zhao,

Wang,

Guo

et al. 2024

BMC Genomics

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Background Quinoa (Chenopodium quinoa Willd.) is valued for its nutritional richness. However, pre-harvest sprouting poses a significant threat to yield and grain quality. This study aims to enhance our understanding of pre-harvest sprouting mitigation strategies, specifically through delayed sowing and avoiding rainy seasons during quinoa maturation. The overarching goal is to identify cold-resistant varieties and unravel the molecular mechanisms behind the low-temperature response of quinoa. We employed bioinformatics and genomics tools for a comprehensive genome-wide analysis of polyamines (PAs) and ethylene synthesis gene families in quinoa under low-temperature stress. Results This involved the identification of 37 PA biosynthesis and 30 PA catabolism genes, alongside 227 ethylene synthesis. Structural and phylogenetic analyses showcased conserved patterns, and subcellular localization predictions indicated diverse cellular distributions. The results indicate that the PA metabolism of quinoa is closely linked to ethylene synthesis, with multiple genes showing an upregulation in response to cold stress. However, differential expression within gene families suggests a nuanced regulatory network. Conclusions Overall, this study contributes valuable insights for the functional characterization of the PA metabolism and ethylene synthesis of quinoa, which emphasize their roles in plant low-temperature tolerance and providing a foundation for future research in this domain.

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Translational and post-translational regulation of polyamine metabolic enzymes in plants

Cited by 7 publications

References 133 publications

The importance of the urea cycle and its relationships to polyamine metabolism during ammonium stress in Medicago truncatula

The importance of the urea cycle and its relationships to polyamine metabolism during ammonium stress in Medicago truncatula

Short-Term Salicylic Acid Treatment Affects Polyamine Metabolism Causing ROS–NO Imbalance in Tomato Roots

Genome-wide identification of polyamine metabolism and ethylene synthesis genes in Chenopodium quinoa Willd. and their responses to low-temperature stress

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