Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in
            <i>Saccharomyces cerevisiae</i>

Stahl, Guillaume; Salem, Samia N. Ben; Chen, Lifeng; Zhao, Bing; Farabaugh, Philip J.

doi:10.1128/ec.3.2.331-338.2004

Cited by 52 publications

(40 citation statements)

References 47 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This switch was likely due to a diauxic growth (Stahl et al 2004, Otterstedt et al 2004Larsson et al 1991, Blomberg et al 1988. During the first part of fermentation the strain grew in a respiro-fermentative mode consuming glucose and producing ethanol and acetate, hence the decrease in pH, and during the second part the strain switched to anaerobic growth with ethanol as carbon source.…”

Section: Batch Culture Results In Microbioreactormentioning

confidence: 99%

Microbioreactors for Bioprocess Development

Zhang

Perozziello

Boccazzi

et al. 2007

JALA: Journal of the Association for Laboratory Automation

View full text Add to dashboard Cite

As a step toward high-throughput bioprocess development, we present design, fabrication, and characterization of polymer based microbioreactors integrated with automated sensors and actuators. The devices are realized, in increasing levels of complexity, in poly(dimethylsiloxane) and poly(methyl methacrylate) by micromachining and multilayer thermal compression bonding procedures. Online optical measurements for optical density, pH, and dissolved oxygen are integrated. Active mixing is made possible by a miniature magnetic stir bar. Plug-in-and-flow microfluidic connectors and fabricated polymer micro-optical lenses/connectors are integrated in the microbioreactors for fast set up and easy operation. Application examples demonstrate the feasibility of culturing microbial cells, specifically Escherichia coli, in 150 μL-volume bioreactors in batch, continuous, and fed-batch operations. (JALA 2007;12:143–51)

show abstract

Section: Batch Culture Results In Microbioreactormentioning

confidence: 99%

Microbioreactors for Bioprocess Development

Zhang

Perozziello

Boccazzi

et al. 2007

JALA: Journal of the Association for Laboratory Automation

View full text Add to dashboard Cite

show abstract

“…Our results indicate that the frameshift rate in Ty2 mRNA also changes in a growth stage-and growth rate-dependent manner. Previously Stahl et al (2004) reported that the translational accuracy and frameshift rate of Ty2 and human immune defi ciency virus type-1 (HIV-1) alter at different points of the growth stages of S. cerevisiae. Our results are also in agreement with these data.…”

Section: Discussionmentioning

confidence: 99%

Glucose Signaling Pathway and Growth Conditions Regulate Gene Expression in Retrotransposon Ty2

Türkel

Bayram

Arik

2009

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Gene expression in the yeast retrotransposon Ty2 is regulated at transcriptional and translational levels. In this study, we have shown that the transcription of Ty2 is partially dependent on the membrane-bound glucose sensors Gpr1p and Mth1p in Saccharomyces cerevisiae. Transcription of Ty2 decreased approx. 3-fold in the gpr1, mth1 yeast mutant. Moreover, our results revealed that the transcription of Ty2 fluctuates during the growth stages of S. cerevisae. Both transcription and the frameshift rate of Ty2 rapidly dropped when the stationary stage yeast cells were inoculated into fresh medium. There was an instant activation of Ty2 transcription and a high level expression during the entire logarithmic stage of yeast growth. However, the transcription of Ty2 decreased 2-fold when the yeast cultures entered the stationary stage. The frameshift rate in Ty2 also varied depending on the growth conditions. The highest frameshift level was observed during the mid-logarithmic stage. It decreased up to 2-fold during the stationary stage. Furthermore, we have found that the frameshift rate of Ty2 diminished at least 5-fold in slowly growing yeasts. These results indicate that the transcription and the frameshift efficiency are coordinately regulated in the retrotransposon Ty2 depending on the growth conditions of S. cerevisiae.

show abstract

“…The decline started in the diauxic phase. The physiological conditions of these cells might affect the translational process that was also required to produce an active heterologous enzyme (Washburne et al, 1993;Stahl et al, 2004;Stanbury et al, 1995;Buchholz et al, 2012). The bglp15.2 enzyme was not entirely secreted by S. cerevisiae BY4741.…”

Section: β-Glucosidasementioning

confidence: 99%

Extracellular β-Glucosidase Production from bglp15.2 Gene Carrying Inulinase Signal Peptide in Saccharomyces cerevisiae BY4741

Fitri¹,

Restiawaty

Moeis³

2017

Jurnal Biodjati

View full text Add to dashboard Cite

One of the important enzymes in cellulase complex is β-glucosidase. In this research, adding signal peptide of inulinase gene from Kluyveromyces marxianus, cloning, and expressing of bglp15.2 gene in S. cerevisiae BY4741 had been done. Gene of bglp15.2 encoding β-glucosidase has 90% identity to nucleotide sequence of Shewanella frigidimarina NCIMB 400 bacteria. Adding nucleotide sequence of signal peptide was aimed to secrete β-glucosidase and had been done with PCR (Polymerase Chain Reaction) method. The addition of nucleotide sequence of signal peptide in bglp15.2 gene had been done succesfully that indicated from nucleotide sequencing result and the increment of amplicon band size in electroferogram of the last addition PCR step. The bglp15.2 and bglp15.2INU gene (the bglp15.2 gene that has signal peptide nucleotide sequence) were cloned in Escherichia coli DH5α using pGEM-T-Easy vector and pBEVY-GL shuttle vector. The pBEVY-GL shuttle vector was used for transforming S. cerevisiae BY4741 with bglp15.2 and bglp15.2INU. The recombinant S. cerevisiae BY4741 carrying bglp15.2INU gene and growing in 48 hours had extracellularly β-glucosidase enzyme activity of 0,0178 U/ml and the intracellularly activity was 0,0181 U/ml. The β-glucosidase enzyme without signal peptide was not secreted. With K. marxianus inulinase signal peptide, about 50% Bglp15.2INU protein could be secreted. The protein molecular weight of secreted Bglp15.2INU was 44 kDa in SDS-PAGE result.

show abstract

Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae

Cited by 52 publications

References 47 publications

Microbioreactors for Bioprocess Development

Microbioreactors for Bioprocess Development

Glucose Signaling Pathway and Growth Conditions Regulate Gene Expression in Retrotransposon Ty2

Extracellular β-Glucosidase Production from bglp15.2 Gene Carrying Inulinase Signal Peptide in Saccharomyces cerevisiae BY4741

Contact Info

Product

Resources

About