1992
DOI: 10.1111/j.1365-2958.1992.tb01561.x
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Translation initiation in Escherichia coli: sequences within the ribosome‐binding site

Abstract: The translational roles of the Shine-Dalgarno sequence, the initiation codon, the space between them, and the second codon have been studied. The Shine-Dalgarno sequence UAAGGAGG initiated translation roughly four times more efficiently than did the shorter AAGGA sequence. Each Shine-Dalgarno sequence required a minimum distance to the initiation codon in order to drive translation; spacing, however, could be rather long. Initiation at AUG was more efficient than at GUG or UUG at each spacing examined; initiat… Show more

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Cited by 321 publications
(277 citation statements)
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References 52 publications
(30 reference statements)
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“…These mutant (SD UP ) ribosome-binding sites were then incorporated into congenic rncЈ-ЈlacZ protein (translational) fusions whose expression was subsequently examined in rnc þ and rnc14::Tn10 cells (note that the rnc14 mutation abolishes RNase III activity; Takiff et al, 1989). In the absence of RNase III (Table 1; rnc14), rnc Ј-ЈlacZ fusion expression increased with SD improvement as expected (Ringquist et al, 1992), but with two exceptions. First, expression from the GGAGG SD mutant was less than that from wild type (Table 1; cf.…”
Section: Increased Rnc Gene Translation Abrogates Rnase III Regulationmentioning
confidence: 84%
“…These mutant (SD UP ) ribosome-binding sites were then incorporated into congenic rncЈ-ЈlacZ protein (translational) fusions whose expression was subsequently examined in rnc þ and rnc14::Tn10 cells (note that the rnc14 mutation abolishes RNase III activity; Takiff et al, 1989). In the absence of RNase III (Table 1; rnc14), rnc Ј-ЈlacZ fusion expression increased with SD improvement as expected (Ringquist et al, 1992), but with two exceptions. First, expression from the GGAGG SD mutant was less than that from wild type (Table 1; cf.…”
Section: Increased Rnc Gene Translation Abrogates Rnase III Regulationmentioning
confidence: 84%
“…Can pseudoknot dependence be overcome by improving the SD sequence of repA? A possible explanation for the absolute requirement of pseudoknot formation for repA translation is that since the predicted SD sequence of repA (UAAGCGA) has relatively weak homology with the consensus sequence (UAAGGAGG) (16), it may be poorly recognized by the ribosome. If so, mutations which strengthen the SD sequence should remove dependence on the pseudoknot.…”
Section: Resultsmentioning
confidence: 99%
“…The optimal spacing for an efficient interaction with the CCUCC core of the E. coli anti-SD region is approx. 7-9 nucleotides, which probably reflects an ideal distance for the subsequent positioning of the initiation codon in the core of the decoding region (Ringquist et al, 1992;La Teana et al, 1995). As most bacterial mRNAs possess an SD signal, it is therefore assumed that the favoured pathway that leads to translation initiation in prokaryotes first starts with SD base pairing ( Fig.…”
Section: Basic Mechanism Of Translation Initiation In Prokaryotesmentioning
confidence: 99%