Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco

Combe, Jonathan P.; Petracek, Marie E.; Eldik, Gerben van; Meulewaeter, Frank; Twell, David

doi:10.1007/s11103-005-3098-x

Cited by 46 publications

(55 citation statements)

References 43 publications

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“…Most importantly, plants possess two quite distinct protein complexes for 5' cap recognition, a classical eIF4F, and a smaller yet more abundant eIFiso4F. Depletion of both eIF4E and eIFiso4E in tobacco with RNA interference results in semi-dwarf phenotypes and reduces polyribosome loading overall (Combe et al, 2005). The reduction in plant growth and development may be attributable to an overall reduction in protein synthesis.…”

Section: Aspects Of Translation Unique To Plantsmentioning

confidence: 99%

Translational Regulation of Cytoplasmic mRNAs

Roy

Arnim

2013

The Arabidopsis Book

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Translation of the coding potential of a messenger RNA into a protein molecule is a fundamental process in all living cells and consumes a large fraction of metabolites and energy resources in growing cells. Moreover, translation has emerged as an important control point in the regulation of gene expression. At the level of gene regulation, translational control is utilized to support the specific life histories of plants, in particular their responses to the abiotic environment and to metabolites. This review summarizes the diversity of translational control mechanisms in the plant cytoplasm, focusing on specific cases where mechanisms of translational control have evolved to complement or eclipse other levels of gene regulation. We begin by introducing essential features of the translation apparatus. We summarize early evidence for translational control from the pre-Arabidopsis era. Next, we review evidence for translation control in response to stress, to metabolites, and in development. The following section emphasizes RNA sequence elements and biochemical processes that regulate translation. We close with a chapter on the role of signaling pathways that impinge on translation.

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Section: Aspects Of Translation Unique To Plantsmentioning

confidence: 99%

Translational Regulation of Cytoplasmic mRNAs

Roy

Arnim

2013

The Arabidopsis Book

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“…33 To examine whether they also contained other transcripts, RT-PCR analysis was performed. Several tobacco pollen-specific genes were tested including profilin, 39 actin depolymerizing factor 1 (ADF1), 40 eukaryotic translation initiation factors eIF4E, eIFiso4E 41 and R-tubulin A1 (accession number AJ421411; D. Brevario, direct submission). RT-PCR analysis revealed that all tested transcripts were present in the EPP fractions isolated by ultracentrifugation as well as directly from SEC ( Figure 3).…”

Section: Epps Contain Different Mrnasmentioning

confidence: 99%

Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization

et al. 2009

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The progamic phase of male gametophyte development involves activation of synthetic and catabolic processes required for the rapid growth of the pollen tube. It is well-established that both transcription and translation play an important role in global and specific gene expression patterns during pollen maturation. On the contrary, germination of many pollen species has been shown to be largely independent of transcription but vitally dependent on translation of stored mRNAs. Here, we report the first structural and proteomic data about large ribonucleoprotein particles (EPPs) in tobacco male gametophyte. These complexes are formed in immature pollen where they contain translationally silent mRNAs. Although massively activated at the early progamic phase, they also serve as a long-term storage of mRNA transported along with the translational machinery to the tip region. Moreover, EPPs were shown to contain ribosomal subunits, rRNAs and a set of mRNAs. Presented results extend our view of EPP complexes from mere RNA storage and transport compartment in particular stages of pollen development to the complex and well-organized machinery devoted to mRNA storage, transport and subsequent controlled activation resulting in protein synthesis, processing and precise localization. Such an organization is extremely useful in fast tip-growing pollen tube. There, massive and orchestrated protein synthesis, processing, and transport must take place in accurately localized regions. Moreover, presented complex role of EPPs in tobacco cytoplasmic mRNA and protein metabolism makes them likely to be active in another plant species too. Expression of vast majority of the closest orthologues of EPP proteins also in Arabidopsis male gametophyte further extends this concept from tobacco to Arabidopsis, the model species with advanced tricellular pollen.

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“…However, an eifiso4g1 eifiso4g2 double mutant displays pleiotropic developmental defaults including dwarfism and reduced fertility 5 . Likewise, silencing of several eIF4E genes in Nicotiana tabacum or Lycopersicon esculentum led to dwarf plants 11 , 12 …”

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confidence: 99%

Pyramiding resistances based on translation initiation factors inArabidopsisis impaired by male gametophyte lethality

Callot

Gallois

2014

Plant Signaling & Behavior

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In eukaryotes, eIF4E translation initiation factors are essential proteins encoded by a small multigene family. In plants, they are a major source of host plant resistance to potyviruses that require specific 4E factors to infect cells. Combining mutations in different eIF4E genes could be a way of broadening the spectrum of plant resistance to viruses. We attempted to combine null mutations affecting the two main Arabidopsis thaliana 4E factors eIF4E1 and eIFiso4E but discovered that this combination is lethal. Transmission through the male gametophyte is completely abolished in the eif4e1 eifiso4e double mutant. This shows that eIF4E1 and eIFiso4E are essential for male gametophyte development and act redundantly. These results may have implications for eIF4E-based pyramiding strategies to improve crop resistance.

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Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco

Cited by 46 publications

References 43 publications

Translational Regulation of Cytoplasmic mRNAs

Translational Regulation of Cytoplasmic mRNAs

Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization

Pyramiding resistances based on translation initiation factors inArabidopsisis impaired by male gametophyte lethality

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