Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine and tyrosine and O-glycosylated in Trichomonas vaginalis

Bifunctional activity of deoxyhypusine synthase/hydroxylase from Trichomonas vaginalis

“…Although, the dimeric (D) rTveIF-5A was observed in a native gel, after two chromatography steps it was impossible to observe these proteins in the 2D gel probably due to monomerization. The pI determined for the rTveIF-5A precursor and mature forms are in agreement with previous reported values [24,26].…”

“…Only organisms in the latelogarithmic-phase were used in the assays. The polyamine metabolism inhibition and restoration was performed as previously reported [26]. After these treatments, parasite viability was checked by the trypan blue (Sigma Chemical Co., St. Louis, MO) exclusion method [27].…”

“…TveIF-5A is phosphorylated and O-glycosylated in the parasite [26,51], these modifications might result in an intermediate TveIF-5A isoform. These observations suggest that polyamines may play an essential role in the posttranslational modification of TveIF-5A by hypusination.…”

The effects of environmental factors on the virulence of Trichomonas vaginalis

“…To date, the presence of PTM has been reported for only a few trichomonad proteins, e.g., TveIF5a [44,66,86], cytoskeletal proteins, tubulin, and several virulence factors, P270, AP120, and TvCP39 [18,21,46]. The hypusination of TveIF-5A lysine residue was demonstrated.…”

Upregulation of eIF-5A1 in the paralyzed muscle after spinal cord transection associates with spontaneous hindlimb locomotor recovery in rats by upregulation of the ErbB, MAPK and neurotrophin signal pathways

“…In humans, they are 84% identical. They are highly conserved throughout eukaryotes and undergo an unusual polyamine-dependent post-translational modification called hypusination [12]. As the only cellular protein that contains the unusual amino acid, hypusine [N(ε)-(4-amino-2-hydroxybutyl) lysine], eIF-5A controls protein synthesis and plays an important role in the regulation of cell proliferation and apoptosis [13][14][15].…”