Translation Inhibition of the Developmental Cycle Protein HctA by the Small RNA IhtA Is Conserved across Chlamydia

Abstract

The developmental cycle of the obligate intracellular pathogen Chlamydia trachomatis serovar L2 is controlled in part by the small non-coding RNA (sRNA), IhtA. All Chlamydia alternate in a regulated fashion between the infectious elementary body (EB) and the replicative reticulate body (RB) which asynchronously re-differentiates back to the terminal EB form at the end of the cycle. The histone like protein HctA is central to RB:EB differentiation late in the cycle as it binds to and occludes the genome, thereby repressing transcription and translation. The sRNA IhtA is a critical component of this regulatory loop as it represses translation of hctA until late in infection at which point IhtA transcription decreases, allowing HctA expression to occur and RB to EB differentiation to proceed. It has been reported that IhtA is expressed during infection by the human pathogens C. trachomatis serovars L2, D and L2b and C. pneumoniae. We show in this work that IhtA is also expressed by the animal pathogens C. caviae and C. muridarum. Expression of HctA in E. coli is lethal and co-expression of IhtA relieves this phenotype. To determine if regulation of HctA by IhtA is a conserved mechanism across pathogenic chlamydial species, we cloned hctA and ihtA from C. trachomatis serovar D, C. muridarum, C. caviae and C. pneumoniae and assayed for rescue of growth repression in E. coli co-expression studies. In each case, co-expression of ihtA with the cognate hctA resulted in relief of growth repression. In addition, expression of each chlamydial species IhtA rescued the lethal phenotype of C. trachomatis serovar L2 HctA expression. As biolayer interferometry studies indicate that IhtA interacts directly with hctA message for all species tested, we predict that conserved sequences of IhtA are necessary for function and/or binding.

Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to InvasiveChlamydia trachomatisBiovars

“…Grieshaber et al showed that the expression of the DNA condensing elementary body (EB)-specific histone-like protein Hc1 is regulated by a transacting sRNA (34). In addition, they showed that the inhibition of HctA translation by lhtA is a conserved function across chlamydiae (33). Intriguingly, in addition to HctA, lhtA also regulates the expression of CTL0322 (CT066) a highly conserved chlamydia-specific hypothetical protein (41).…”

A Stress-Induced Small RNA Modulates Alpha-Rhizobial Cell Cycle Progression

“…trans -sRNA Pxr negatively regulates fruiting body formation in Myxococcus [ 64 ]. In Chlamydia , the conserved IhtA sRNA translationally inhibits the histone-like protein Hc1 that is involved in compaction of the chromatin into metabolically inert forms during host infection [ 65 , 66 ]. In E .…”

Chlamydia trachomatis In Vivo to In Vitro Transition Reveals Mechanisms of Phase Variation and Down-Regulation of Virulence Factors

“…Although increasing data have shown that the expression of sRNAs may be determinant for virulence in both extracellular [ 140 ] and intracellular bacterial pathogens [ 141 ] and, in C . trachomatis , one sRNA (lhtA) was found to control the timing of RB to EB transition [ 142 , 143 ], the specific role of the sRNA ctrR0332 on C . trachomatis virulence cannot be determined yet.…”