Translating rodent behavioral repertoire to zebrafish (Danio rerio): Relevance for stress research

Champagne, Danielle L.; Hoefnagels, Carlijn C.M.; Kloet, E. Ronald de; Richardson, Michael K.

doi:10.1016/j.bbr.2010.06.001

Cited by 340 publications

(231 citation statements)

References 64 publications

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“…We were able to obtain only a small number of spawns for tagged and untagged fish, which was probably due to the combination of two factors: frequent handling under anaesthesia at fortnightly intervals and restrictively low food levels. Analysis of the swimming activity in a T-maze indicated that swimming ability as measured 2months after tagging was not modified by tagging and that tagging did not induce modification of behavioural traits that could have been interpreted as enforced anxiety (Champagne et al, 2010).…”

Section: Tagging Success and Effectsmentioning

confidence: 98%

“…Behaviour is now recognized as an integrative indicator of both organism stress response and physiological alterations (Champagne et al, 2010;Steenbergen et al, 2011). Two months after tagging, we compared the swimming activity of tagged fish with that of Tagged fish were grouped by sex and mass classes at tagging (100mg classes, except the largest class for female, which ranges from 651 to 850mg).…”

Section: Swimming Behaviourmentioning

confidence: 99%

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Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Cousin

Daouk

Péan

et al. 2012

Journal of Experimental Biology

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SUMMARYAlthough individual electronic tagging using passive integrated acoustic (PIT) tags is established, it is mainly for fish >60mm in length and is unsuitable for fish of <30mm, like zebrafish. We used radio frequency identification (RFID) microtags (1mm in diameter and 6mm in length, with a mass of ~10mg) to individually identify juvenile zebrafish (length 16-42mm, mass 138-776mg) for the first time, and studied the effects of intracoelomic implantation on fish survival and microtag loss, growth, spawning and exploratory behaviour. After 5.5months, both high survival (82%) and low microtag loss (11%) were achieved. The smallest surviving fish weighed 178mg, and success in microtag reading was 73% for the size class 350-450mg (26mm). Greater success was achieved when fish were larger at the time of tagging but no negative effects on growth were observed for any size class and some tagged fish spawned. No significant differences in behavioural responses could be detected between tagged fish and untagged controls after 2months. Overall, the results suggest that the tagging method is highly suitable for fish as small as zebrafish juveniles. We think this method will provide significant advances for researchers of the ever-growing fish model community and more generally for all small-fish users. Tagging is essential when one needs to identify fish (e.g. particular genotypes with no external cue), to run longitudinal monitoring of individual biological traits (e.g. growth) or to repeat assays with the same individual at discrete points in time (e.g. behaviour studies). Such a method will find applications in physiology, genetics, behaviour and (eco)toxicology fields. Supplementary material available online at

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Section: Tagging Success and Effectsmentioning

confidence: 98%

Section: Swimming Behaviourmentioning

confidence: 99%

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Cousin

Daouk

Péan

et al. 2012

Journal of Experimental Biology

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“…The zebrafish is an increasingly accepted model organism to study neurological disorders because of its similarity to humans with regard to neurotransmitter systems and stress axis organization [12]. It combines easy handling and relatively high-throughput screening with the complexity of a whole vertebrate organism.…”

Section: Introductionmentioning

confidence: 99%

Cross-species pharmacological characterization of the allylglycine seizure model in mice and larval zebrafish

Leclercq

Afrikanova

Langlois

et al. 2015

Epilepsy & Behavior

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Treatment-resistant seizures affect about a third of patients suffering from epilepsy. To fulfill the need for new medications targeting treatment-resistant seizures, a number of rodent models offer the opportunity to assess a variety of potential treatment approaches. The use of such models, however, has proven to be timeconsuming and labor-intensive. In this study, we performed pharmacological characterization of the allylglycine (AG) seizure model, a simple in vivo model for which we demonstrated a high level of treatment resistance. (D,L)-Allylglycine inhibits glutamic acid decarboxylase (GAD) -the key enzyme in γ-aminobutyric acid (GABA) biosynthesis -leading to GABA depletion, seizures, and neuronal damage. We performed a side-by-side comparison of mouse and zebrafish acute AG treatments including biochemical, electrographic, and behavioral assessments. Interestingly, seizure progression rate and GABA depletion kinetics were comparable in both species. Five mechanistically diverse antiepileptic drugs (AEDs) were used. Three out of the five AEDs (levetiracetam, phenytoin, and topiramate) showed only a limited protective effect (mainly mortality delay) at doses close to the TD 50 (dose inducing motor impairment in 50% of animals) in mice. The two remaining AEDs (diazepam and sodium valproate) displayed protective activity against AG-induced seizures. Experiments performed in zebrafish larvae revealed behavioral AED activity profiles highly analogous to those obtained in mice. Having demonstrated crossspecies similarities and limited efficacy of tested AEDs, we propose the use of AG in zebrafish as a convenient and high-throughput model of treatment-resistant seizures.

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“…Although there is evidence that zebrafish may be suitable to study neurobiology of human pathologies, the assessment of CUS induced apoptotic pathway in this model is scarcely characterized 26 . Therefore the purpose of the study is to establish a CUS zebrafish model.…”

Section: Introductionmentioning

confidence: 99%

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2017

IJPSR

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Stress plays a major role in various pathophysiological processes related with neurodegenerative diseases and mental disorders. Zebrafish has emerged as a promising model organism in inducing Chronic Unpredictable Stress (CUS). In the present study, we have attempted to understand the molecular basis of neuronal changes underlying CUS in zebrafish model. An evidence for a connection between CUS and apoptosis in the brain of zebrafish was analyzed. For this purpose, zebrafish were subjected to CUS protocol twice a day for a period of 15 days. The impact of CUS on the brain was evaluated in relation to the expression of the stress marker genes: corticotropin releasing factor (crf) and glucocorticoid receptor (gr). The effects of CUS were also estimated from the expression of p53 mediated apoptotic genes: p53, noxa, bcl2 and casp3. CUS protocol increased the gene expression of crf (p<0.05) and decreased the expression of gr (p<0.05). The impact of CUS on the apoptotic pathway showed increased expression of p53 (p< 0.05) and noxa (p<0.05) with a decrease in expression of mitochondrial bcl2 (p<0.05). As a hallmark of apoptosis, CUS increased the expression of casp3 (p<0.05). In principle, CUS has the potency to exert their detrimental effects on the neuronal cells of the brain and has confirmed p53 induced apoptosis in the brain of zebrafish.

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Translating rodent behavioral repertoire to zebrafish (Danio rerio): Relevance for stress research

Cited by 340 publications

References 64 publications

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Cross-species pharmacological characterization of the allylglycine seizure model in mice and larval zebrafish

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