Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium

Ge, Haitao; Fang, Lichun; Huang, Xiahe; Wang, Jinlong; Chen, Weiyang; Liu, Ye; Zhang, Yuanya; Wang, Xiaorong; Xu, Wu; He, Qingfang; Wang, Yingchun

doi:10.1074/mcp.m117.068080

Cited by 3 publications

(1 citation statement)

References 76 publications

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“…It is reasonable that organisms would prioritize survival when subjected to stress. For example, when stress occurs in cyanobacteria, photosynthesis is usually downregulated, whereas the stress response is upregulated (Ge et al ., ). Similarly, decline of photosynthesis is the primary effect of water deficit, and the secondary effect would occur along with continuous drought stress (Chaves et al ., ).…”

Section: Discussionmentioning

confidence: 97%

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

et al. 2019

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† These authors contributed equally to this work. SUMMARYWRKY transcription factors play important roles in response to various abiotic stresses. Previous study have proved that soybean GmWRKY54 can improve stress tolerance in transgenic Arabidopsis. Here, we generated soybean transgenic plants and further investigated roles and biological mechanisms of GmWRKY54 in response to drought stress. We demonstrated that expression of GmWRKY54, driven by either a constitutive promoter (pCm) or a drought-induced promoter (RD29a), confers drought tolerance. GmWRKY54 is a transcriptional activator and affects a large number of stress-related genes as revealed by RNA sequencing. Gene ontology (GO) enrichment and co-expression network analysis, together with measurement of physiological parameters, supported the idea that GmWRKY54 enhances stomatal closure to reduce water loss, and therefore confers drought tolerance in soybean. GmWRKY54 directly binds to the promoter regions of genes including PYL8, SRK2A, CIPK11 and CPK3 and activates them. Therefore GmWRKY54 achieves its function through abscisic acid (ABA) and Ca 2+ signaling pathways. It is valuable that GmWRKY54 activates an ABA receptor and an SnRK2 kinase in the upstream position, unlike other WRKY proteins that regulate downstream genes in the ABA pathway. Our study revealed the role of GmWRKY54 in drought tolerance and further manipulation of this gene should improve growth and production in soybean and other legumes/crops under unfavorable conditions.

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Section: Discussionmentioning

confidence: 97%

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

et al. 2019

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Functional proteomics reveals that Slr0237 is a SigE‐regulated glycogen debranching enzyme pivotal for glycogen breakdown

Liu,

Ge,

2024

Proteomics

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The group 2 σ factor for RNA polymerase SigE plays important role in regulating central carbon metabolism in cyanobacteria. However, the regulation of SigE for these pathways at a proteome level remains unknown. Using a sigE‐deficient strain (ΔsigE) of Synechocystis sp. PCC 6803 and quantitative proteomics, we found that SigE depletion induces differential protein expression for sugar catabolic pathways including glycolysis, oxidative pentose phosphate (OPP) pathway, and glycogen catabolism. Two glycogen debranching enzyme homologues Slr1857 and Slr0237 are found differentially expressed in ΔsigE. Glycogen determination indicated that Δslr0237 accumulated glycogen under photomixotrophic condition but was unable to utilize these reserves in the dark, whereas Δslr1857 accumulates and utilizes glycogen in a similar way as the WT strain does in the same condition. These results suggest that Slr0237 plays the major role as the glycogen debranching enzyme in Synechocystis.

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Deep Proteogenomics of a Photosynthetic Cyanobacterium

et al. 2023

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Cyanobacteria, the evolutionary ancestors of plant chloroplasts, contribute substantially to the Earth's biogeochemical cycles and are of great interest for a sustainable economy. Knowledge of protein expression is the key to understanding cyanobacterial metabolism; however, proteome studies in cyanobacteria are limited and cover only a fraction of the theoretical proteome.Here, we performed a comprehensive proteogenomic analysis of the model cyanobacterium Synechocystis sp. PCC 6803 to characterize the expressed (phospho)proteome, re-annotate known and discover novel open reading frames (ORFs). By mapping extensive shotgun mass spectrometry proteomics data onto a six-frame translation of the Synechocystis genome, we refined the genomic annotation of 64 ORFs, including eight completely novel ORFs. Our study presents the largest reported (phospho)proteome dataset for a unicellular cyanobacterium, covering the expression of about 80% of the theoretical proteome under various cultivation conditions, such as nitrogen or carbon limitation. We report 568 phosphorylated S/T/Y sites that are present on numerous regulatory proteins, including the transcriptional regulators cyAbrB1 and cyAbrB2. We also catalogue the proteins that have never been detected under laboratory conditions and found that a large portion of them is plasmid-encoded. This dataset will serve as a resource, providing dedicated information on growth condition-dependent protein expression and phosphorylation.

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Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium

Cited by 3 publications

References 76 publications

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

Functional proteomics reveals that Slr0237 is a SigE‐regulated glycogen debranching enzyme pivotal for glycogen breakdown

Deep Proteogenomics of a Photosynthetic Cyanobacterium

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Translating Divergent Environmental Stresses into a Common Proteome Response through Hik33 in a Model Cyanobacterium

Cited by 3 publications

References 76 publications

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca2+ signaling pathways in transgenic soybean

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca2+ signaling pathways in transgenic soybean

Functional proteomics reveals that Slr0237 is a SigE‐regulated glycogen debranching enzyme pivotal for glycogen breakdown

Deep Proteogenomics of a Photosynthetic Cyanobacterium

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Resources

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GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean