Transitory Increase of Hematoencephalic Barrier Permeability by Intracarotid Introduction of Ozonized Saline Solution

Abstract

The aim of the investigation was to assess experimentally the effect of ozonized saline solution with various concentrations of ozone on the dynamics of hematoencephalic barrier (HEB) permeability.Materials and Methods. Investigation of the ultrastructure of the sensorimotor zone of animal cerebral cortex to assess HEB state was carried out on mature outbred Wistar rats (n=93). In the control series, 0.9% saline solution was introduced intracarotidly, in the experimental series ozonized saline solution (OSS) at 0.7 and 3.5 mg/L ozone concentration was injected. The tissue of the rat sensorimotor cerebral cortex was examined morphologically 15 min, 1, 14 and 30 days after the solution introduction. The analysis of HEB permeability was evaluated by the state of astroglia, basement membrane, and intercellular junctions of capillary endothelium.Results. It has been established that 15 min after a single introduction of OSS with 0.7 mg/L ozone concentration, a transitory disruption of HEB permeability took place, which was manifested by partial reduction of interendothelial tight junctions, widening of intercellular gaps, swelling of the basement membrane and part of the astrocyte processes in the pericapillary area. Neural ultrastructure alterations were of the adaptive character in response to the effect of the oxidative factor, and were characterized by the increase of protein-synthesizing organelle activity. A day after OSS action, increase of HEB permeability grew, on day 14 the HEB structure was partly normalized (capillary endothelium had tight intercellular junctions), and by day 30 the HEB ultrastructure and microcirculation had been restored. The increase of ozone concentration to 3.5 mg/L in the intracarotidly introduced OSS also resulted in the rise of HEB permeability, however, its restoration by day 30 was not observed. Besides, alterations of the microcirculatory blood vessels, neuron ultrastructure, and glias were more pronounced.Conclusion. A single intracarotid introduction of OSS with 0.7 mg/L ozone concentration increases HEB permeability over 1 day period, which can provide a more effective influence of preparations on the brain cells in all phases of the cell cycle.