Transitions in nitrogen and organic matter form and concentration correspond to bacterial population dynamics in a hypoxic urban estuary

Abstract

Nitrogen (N) inputs to developed coastlines are linked with multiple ecosystem and socio-economic impacts worldwide such as algal blooms, habitat/resource deterioration, and hypoxia. This study investigated the microbial and biogeochemical processes associated with recurrent, seasonal bottom-water hypoxia in an urban estuary, western Long Island Sound (WLIS), that receives high N inputs. A 2-year (2020–2021) field study spanned two hypoxia events and entailed surface and bottom depth water sampling for dissolved nutrients as inorganic N (DIN; ammonia-N and nitrite + nitrate (N + N)), organic N, orthophosphate, organic carbon (DOC), as well as chlorophyll a and bacterial abundances. Physical water quality data were obtained from concurrent conductivity, temperature, and depth casts. Results showed that dissolved organic matter was highest at the most-hypoxic locations, DOC was negatively and significantly correlated with bottom-water dissolved oxygen (Pearson’s r = −0.53, p = 0.05), and ammonia-N was the dominant DIN form pre-hypoxia before declining throughout hypoxia. N + N concentrations showed the reverse, being minimal pre-hypoxia then increasing during and following hypoxia, indicating that ammonia oxidation likely contributed to the switch in dominant DIN forms and is a key pathway in WLIS water column nitrification. Similarly, at the most hypoxic sampling site, bottom depth bacteria concentrations ranged ~ 1.8 × 10 4 –1.1 × 10 5 cells ml −1 pre-hypoxia, declined throughout hypoxia, and were positively and significantly correlated (Pearson’s r = 0.57; p = 0.03) with ammonia-N, confirming that hypoxia influences N-cycling within LIS. These findings provide novel insight to feedbacks between major biogeochemical (N and C) cycles and hypoxia in urban estuaries. Supplementary Information The online version contains supplementary material available at 10.1007/s10533-023-01021-2.