Transition to HBeAg-negative chronic hepatitis B virus infection is associated with reduced cccDNA transcriptional activity

Suslov, Aleksei; Meier, Mario; Ketterer, Sylvia; Wang, Xueya; Wieland, Stefan; Heim, Markus H.

doi:10.1016/j.jhep.2020.11.003

Cited by 53 publications

(61 citation statements)

References 30 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supportively, it has been suggested that HBeAg seroconversion may reduce the transcription efficiency of cccDNA by epigenetic silence and mutation accumulation of cccDNA. ( 3,4 )…”

Section: Figmentioning

confidence: 99%

Letter to the Editor: The Differences Between the Reverse Transcriptional Efficiency of HBV Pregenomic RNA and Transcriptional Efficiency of HBV Covalently Closed Circular DNA

et al. 2021

View full text Add to dashboard Cite

“…Supportively, it has been suggested that HBeAg seroconversion may reduce the transcription efficiency of cccDNA by epigenetic silence and mutation accumulation of cccDNA. ( 3,4 )…”

Section: Figmentioning

confidence: 99%

Letter to the Editor: The Differences Between the Reverse Transcriptional Efficiency of HBV Pregenomic RNA and Transcriptional Efficiency of HBV Covalently Closed Circular DNA

et al. 2021

View full text Add to dashboard Cite

“…Uncoupling of viral replication and HBsAg expression from cccDNA could be a reason [8,9]. Alternatively, HBsAg expression from integrated HBV DNA could preserve HBsAg expression despite reduced viral replication in HBeAg negative disease stages [3].…”

Section: Introductionmentioning

confidence: 99%

Ubiquitous expression of HBsAg from integrated HBV DNA in patients with low viral load

Meier

Calabrese

Suslov

et al. 2021

Journal of Hepatology

Self Cite

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

“…These findings suggest that the transcriptional activity of HBV cccdna may decrease in the late stage of infection. Indeed, recent studies confirm transcriptional suppression of cccdna in HBeag-negative chronic hepatitis B (5,6). The transcriptional activity of HBV cccdna is controlled by four promoters (precore/pregenomic, S1, S2 and X) and two enhancer sequences (enhancer I and enhancer ii) (7).…”

Section: Introductionmentioning

confidence: 89%

Methylation profile of hepatitis B virus is not influenced by interferon α in human liver cancer cells

Moon

Kim

2021

Mol Med Rep

View full text Add to dashboard Cite

Interferon (IFN) α is used for the treatment of chronic hepatitis B virus (HBV) infection, but the molecular mechanisms underlying its antiviral effect have not been fully elucidated. Epigenetic modifications regulate the transcriptional activity of covalently closed circular DNA (cccDNA) in cells with chronic HBV infection. IFN-α has been shown to modify cccDNA-bound histones, but it is not known whether the anti-HBV effect of IFN-α involves methylation of cccDNA. The present study aimed to determine whether IFN-α induced methylation of HBV cccDNA in a cell-based model in which HepG2 cells were directly infected with wild-type HBV virions. Methylation status of HBV cccDNA was assessed using global DNA methylation ELISA assay, methylation-specific PCR and bisulfite sequencing. IFN-α suppressed HBV DNA and RNA transcripts, but methylation profiles were similar between the control and IFN-α treated groups. Chromatin immunoprecipitation results revealed binding of DNA methyltransferases (DNMT) 3A and DNMT3B to HBV cccDNA and treatment with IFN-α suppressed the recruitment of DNMT3B to cccDNA. Taken together, these results suggest that IFN-α does not induce methylation of HBV cccDNA. Therefore, it was concluded that methylation is unlikely to contribute to the anti-HBV effect of IFN-α in HepG2 cells, and that alternative mechanisms need to be sought to enhance cccDNA methylation as a novel therapy against HBV.

show abstract

Transition to HBeAg-negative chronic hepatitis B virus infection is associated with reduced cccDNA transcriptional activity

Cited by 53 publications

References 30 publications

Letter to the Editor: The Differences Between the Reverse Transcriptional Efficiency of HBV Pregenomic RNA and Transcriptional Efficiency of HBV Covalently Closed Circular DNA

Letter to the Editor: The Differences Between the Reverse Transcriptional Efficiency of HBV Pregenomic RNA and Transcriptional Efficiency of HBV Covalently Closed Circular DNA

Ubiquitous expression of HBsAg from integrated HBV DNA in patients with low viral load

Methylation profile of hepatitis B virus is not influenced by interferon α in human liver cancer cells

Contact Info

Product

Resources

About