Transition period-related changes in the abundance of the mRNAs of adiponectin and its receptors, of visfatin, and of fatty acid binding receptors in adipose tissue of high-yielding dairy cows

Lemor, Anneka; Hosseini, Afshin; Sauerwein, H.; Mielenz, M.

doi:10.1016/j.domaniend.2009.01.004

Cited by 67 publications

(81 citation statements)

References 55 publications

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“…Several factors may have attributed to the differences in gene expression results between Ji et al (2012) and our studies, such as breed, composition of diet, level and duration of overfeeding, and degree of fatness. In the current study, lower expression of ADI-POQ receptors in HE compared with CON cows may have reduced ADIPOQ sensitivity; thus, reduced insulin sensitivity in SAT of HE may be expected (Lemor et al, 2009). The lack of changes in the expression of ADIPOQ and its receptors from pregnancy to early lactation was consistent with previous studies (Giesy et al, 2012;Selim et al, 2014).…”

Section: Effect Of Overfeeding On Sat Gene Expressionsupporting

confidence: 89%

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Selim

Kokkonen

Taponen

et al. 2015

Journal of Dairy Science

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Prepartal energy overfeeding may predispose cows to a state of increased insulin resistance with greater lipolysis after parturition. The aim of the study was to evaluate the effects of prepartal overfeeding in terms of abundant grass silage ration on the liver and subcutaneous adipose tissue (SAT) gene expression around parturition. Sixteen multiparous Finnish Ayrshire dairy cows were fed ad libitum either grass silage [high energy, HE; 144 MJ/d of metabolizable energy (ME) intake, n=8] or a mixture of grass silage, wheat straw, and rapeseed meal [55:40:5 (CON), 109 MJ/d of ME, n=8] during the dry period (58.2±4.89 d, mean ± standard deviation). Tissue biopsies and blood samples were collected at -14 (±4.98), 1, and 7 d relative to the actual parturition date. The HE cows had greater total dry matter intake, ME intake, and ME balance during the dry period than the CON cows. Compared with CON, the increases in body weight and body condition score were greater in HE during the dry period. Milk yield during the first 2 wk of lactation was not different between the groups. Plasma glucose, nonesterified fatty acids, insulin, glucagon, and β-hydroxybutyrate did not differ between the groups during the transition period. Dietary treatment did not affect hepatic triglyceride content; however, a delayed increase in hepatic total lipid content was observed in the HE cows at d 1 postpartum. Hepatic cytosolic phosphoenolpyruvate carboxykinase 1 mRNA expression was lower in HE than in CON at d 1 and 7 postpartum. Adiponectin receptor 1 and 2 mRNA abundance tended to be lower in SAT of HE than CON. Lower lipoprotein lipase, leptin, and stearoyl-coenzyme A desaturase mRNA abundances were observed at d 7 postpartum in SAT of the HE cows compared with the CON cows. We concluded that prepartal ad libitum feeding of grass silage may decrease insulin sensitivity and lipogenesis in SAT during peripartal period and may attenuate the increase of hepatic gluconeogenic capacity from propionate compared with a controlled-energy diet.

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Section: Effect Of Overfeeding On Sat Gene Expressionsupporting

confidence: 89%

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Selim

Kokkonen

Taponen

et al. 2015

Journal of Dairy Science

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“…(6,8,9) In cattle, the role of the adipokines like AdipoQ in lipid metabolism, insulin resistance, and glucose homeostasis is not clear. (10) Lemor and colleagues (10) have shown decreasing AdipoQ sensitivity in adipose tissue after calving. Wang and colleagues (11) observed that specifically increasing HMW AdipoQ concentrations in the liver (compared to total AdipoQ) correlate well with improved insulin resistance.…”

Section: Introductionmentioning

confidence: 98%

A Monoclonal Antibody Against Bovine Adiponectin

et al. 2012

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Adiponectin (AdipoQ) is an adipokine mainly secreted by white fatty tissue, playing a major role in energy homeostasis and insulin sensitivity. For cattle, AdipoQ data are largely limited to mRNA expression; to our knowledge, valid information about the AdipoQ protein in bovine tissues and body fluids is not available. Therefore, we have developed a monoclonal antibody against bovine AdipoQ. This study describes the preparation, application, and characterization of a monoclonal antibody for use in ELISA, Western blot, and histology. The antibody was developed by PEG fusion of the SP2/0 cell line with splenic B cells from AdipoQ immunized C57Bl/6 mice. Antibody-producing cells were identified by ELISA and specified by immunoblotting and immunostaining of bovine retroperitoneal adipose tissue. The novel antibody detects AdipoQ in histological samples, ELISA, and Western blots.

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“…In another study we found a decrease of the receptor mRNA abundance from late pregnancy (day −21) to mid-(day 105) and late-lactation (day 252 relative to calving), but also not in the time interval considered in the current study (Lemor et al, 2009;Friedrichs et al, 2014). We thus assume that the expressional regulation of HCAR2 and the feedback of BHBA on lipolysis via this receptor are only relevant if BHBA concentrations are in a range observed during ketosis.…”

Section: Changes In Hcar2 Expressionmentioning

confidence: 93%

Expression of metabolic sensing receptors in adipose tissues of periparturient dairy cows with differing extent of negative energy balance

Friedrichs

Sauerwein

Huber

et al. 2016

animal

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We recently showed that the mRNA expression of genes encoding for specific nutrient sensing receptors, namely the free fatty acid receptors (FFAR) 1, 2, 3, and the hydroxycarboxylic acid receptor (HCAR) 2, undergo characteristic changes during the transition from late pregnancy to lactation in certain adipose tissues (AT) of dairy cows. We hypothesised that divergent energy intake achieved by feeding diets with either high or low portions of concentrate (60% v. 30% concentrate on a dry matter basis) will alter the mRNA expression of FFAR 1, 2, 3, as well as HCAR2 in subcutaneous (SCAT) and retroperitoneal AT (RPAT) of dairy cows in the first 3 weeks postpartum (p.p.). For this purpose, 20 multiparous German Holstein cows were allocated to either the high concentrate ration (HC, n = 10) or the low concentrate ration (LC, n = 10) from day 1 to 21 p.p. Serum samples and biopsies of SCAT (tail head) and RPAT (above the peritoneum) were obtained at day −21, 1 and 21 relative to parturition. The mRNA abundances were measured by quantitative PCR. The concentrations of short-chain fatty acid (SCFA) in serum were measured by gas chromatography-flame ionisation detector. The FFAR1 and FFAR2 mRNA abundance in RPAT was higher at day −21 compared to day 1. At day 21 p.p. the FFAR2 mRNA abundance was 2.5-fold higher in RPAT of the LC animals compared to the HC cows. The FFAR3 mRNA abundance tended to lower values in SCAT of the LC group at day 21. The HCAR2 mRNA abundance was neither affected by time nor by feeding in both AT. On day 21 p.p. the HC group had 1.7-fold greater serum concentrations of propionic acid and lower concentrations of acetic acid (trend: 1.2-fold lower) compared with the LC group. Positive correlations between the mRNA abundance of HCAR2 and peroxisome proliferator-activated receptor γ-2 (PPARG2) indicate a link between HCAR2 and PPARG2 in both AT. We observed an inverse regulation of FFAR2 and FFAR3 expression over time and both receptors also showed an inverse mRNA abundance as induced by different portions of concentrate. Thus, indicating divergent nutrient sensing of both receptors in AT during the transition period. We propose that the different manifestation of negative EB in both groups at day 21 after parturition affect at least FFAR2 expression in RPAT.Keywords: transition period, bovine adipose tissue, metabolic sensing ImplicationsAt the onset of lactation, the feed intake does not increase to the same extent as the energy requirements for milk synthesis resulting in a negative energy balance in high-yielding dairy cows. To cover the energy requirements of increasing milk synthesis, the mobilisation of energy from adipose tissue is crucial during the peripartal period. We studied the effects of two different concentrate portions in the feeding ration on the transcriptional regulation of four different nutrient sensing receptors, involved in adipose tissue metabolism. We used adipose tissue from a subcutaneous and a visceral localisation. The findings of our study may help to understand, in pa...

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Transition period-related changes in the abundance of the mRNAs of adiponectin and its receptors, of visfatin, and of fatty acid binding receptors in adipose tissue of high-yielding dairy cows

Cited by 67 publications

References 55 publications

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

A Monoclonal Antibody Against Bovine Adiponectin

Expression of metabolic sensing receptors in adipose tissues of periparturient dairy cows with differing extent of negative energy balance

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