Transition metal distribution in the brain and spinal cord of a dysmyelinated rodent model

Al-Ebraheem, Alia; Miller, Lauren A.; Geraki, Kalotina; Desmond, Kimberly L.; Stanisz, Greg J.; Bock, Nicholas A.; Farquharson, Michael J.

doi:10.1063/1.4961139

Cited by 2 publications

(5 citation statements)

References 33 publications

(34 reference statements)

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“…The quantitative metal measurements are presented in more detail in a companion work by Al‐Ebraheem et al . .…”

Section: Resultsmentioning

confidence: 99%

“…and les in white matter and molecular and granular regions of cerebellar cortex, please refer to the companion work by Al‐Ebraheem et al . .…”

Section: Resultsmentioning

confidence: 99%

“…We did not use the values of metals other than Fe or Mn for further analysis within this work, but Table 1 also includes the concentrations of Zn, Ca and Cu for completeness. The quantitative metal measurements are presented in more detail in a companion work by Al-Ebraheem et al (55).…”

Section: Histologymentioning

confidence: 99%

“…Average MRI (R 1 and R 2 ) and metal measurements (concentration of Fe, Mn, Zn, Cu and Ca) in regions of interest (ROIs) in the deep cerebellar white matter of Long Evans wild-type (wt) and Long Evans Shaker (les) rats. Further details of the metal quantification can be found in Al-Ebraheem et al (55). Parameter intervals are the standard deviation of the mean ROI value from all animals.…”

Section: Theoretical Contribution Of Fe and Mn To The Observed Relaxamentioning

confidence: 99%

“…For further details regarding the metal maps, and comparisons between wt. and les in white matter and molecular and granular regions of cerebellar cortex, please refer to the companion work by Al-Ebraheem et al(55).…”

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Differences in iron and manganese concentration may confound the measurement of myelin fromR₁andR₂relaxation rates in studies of dysmyelination

et al. 2016

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A model of dysmyelination, the Long Evans Shaker (les) rat, was used to study the contribution of myelin to MR tissue properties in white matter. A large region of white matter was identified in the deep cerebellum and was used for measurements of the MR relaxation rate constants, R1 = 1/T1 and R2 = 1/T2 , at 7 T. In this study, R1 of the les deep cerebellar white matter was found to be 0.55 ± 0.08 s (-1) and R2 was found to be 15 ± 1 s(-1) , revealing significantly lower R1 and R2 in les white matter relative to wild-type (wt: R1 = 0.69 ± 0.05 s(-1) and R2 = 18 ± 1 s(-1) ). These deviated from the expected ΔR1 and ΔR2 values, given a complete lack of myelin in the les white matter, derived from the literature using values of myelin relaxivity, and we suspect that metals could play a significant role. The absolute concentrations of the paramagnetic transition metals iron (Fe) and manganese (Mn) were measured by a micro-synchrotron radiation X-ray fluorescence (μSRXRF) technique, with significantly greater Fe and Mn in les white matter than in wt (in units of μg [metal]/g [wet weight tissue]: les: Fe concentration,19 ± 1; Mn concentration, 0.71 ± 0.04; wt: Fe concentration,10 ± 1; Mn concentration, 0.47 ± 0.04). These changes in Fe and Mn could explain the deviations in R1 and R2 from the expected values in white matter. Although it was found that the influence of myelin still dominates R1 and R2 in wt rats, there were non-negligible changes in the contribution of the metals to relaxation. Although there are already problems with the estimation of myelin from R1 and R2 changes in disease models with pathology that also affects the relaxation rate constants, this study points to a specific pitfall in the estimation of changes in myelin in diseases or models with disrupted concentrations of paramagnetic transition metals. Copyright © 2016 John Wiley & Sons, Ltd.

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“…The quantitative metal measurements are presented in more detail in a companion work by Al‐Ebraheem et al . .…”

Section: Resultsmentioning

confidence: 99%

“…and les in white matter and molecular and granular regions of cerebellar cortex, please refer to the companion work by Al‐Ebraheem et al . .…”

Section: Resultsmentioning

confidence: 99%

Section: Histologymentioning

confidence: 99%

Section: Theoretical Contribution Of Fe and Mn To The Observed Relaxamentioning

confidence: 99%

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confidence: 99%

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Differences in iron and manganese concentration may confound the measurement of myelin fromR₁andR₂relaxation rates in studies of dysmyelination

et al. 2016

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Trace elements dyshomeostasis in liver and brain of weanling mice under altered dietary selenium conditions

Sharma,

Mohanty,

Singh

et al. 2023

Journal of Trace Elements in Medicine and Biology

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Transition metal distribution in the brain and spinal cord of a dysmyelinated rodent model

Cited by 2 publications

References 33 publications

Differences in iron and manganese concentration may confound the measurement of myelin fromR₁andR₂relaxation rates in studies of dysmyelination

Differences in iron and manganese concentration may confound the measurement of myelin fromR₁andR₂relaxation rates in studies of dysmyelination

Trace elements dyshomeostasis in liver and brain of weanling mice under altered dietary selenium conditions

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Transition metal distribution in the brain and spinal cord of a dysmyelinated rodent model

Cited by 2 publications

References 33 publications

Differences in iron and manganese concentration may confound the measurement of myelin fromR1andR2relaxation rates in studies of dysmyelination

Differences in iron and manganese concentration may confound the measurement of myelin fromR1andR2relaxation rates in studies of dysmyelination

Trace elements dyshomeostasis in liver and brain of weanling mice under altered dietary selenium conditions

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Differences in iron and manganese concentration may confound the measurement of myelin fromR₁andR₂relaxation rates in studies of dysmyelination

Differences in iron and manganese concentration may confound the measurement of myelin fromR₁andR₂relaxation rates in studies of dysmyelination