Transition from maternal to embryonic control of development in IVM/IVF domestic cat embryos

Hoffert, Kara A.; Anderson, G. B.; Wildt, David E.; Roth, Terri L.

doi:10.1002/(sici)1098-2795(199710)48:2<208::aid-mrd8>3.0.co;2-w

Cited by 46 publications

(30 citation statements)

References 43 publications

(62 reference statements)

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“…In contrast to bovine oocytes, we found that porcine oocytes were able to support nucleoli formation in nuclei of many species: equine, rabbit, canine, and feline that possess very close EGA at the 4-to 8-cell stage (Grøndahl & Hyttel 1996, Hoffert et al 1997, Kanka 2003, Maddox-Hyttel et al 2005, and, interestingly, even in some ovine nuclei with EGA at the 8-cell stage (Crosby et al 1988). The most intensively nucleolinlabeled nucleoli were found in equine and rabbit nuclei.…”

Section: Discussioncontrasting

confidence: 61%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has been achieved in closely related species. The analyses of embryonic gene activity in iSCNT embryos of different species combinations have revealed the existence of significant aberrations in expression of housekeeping genes and genes dependent on the major embryonic genome activation (EGA). However, there are many studies with successful blastocyst (BL) development of iSCNT embryos derived from donor cells and oocytes of animal species with distant taxonomical relations (inter-family/inter-class) that should indicate proper EGA at least in terms of RNA polymerase I activation, nucleoli formation, and activation of genes engaged in morula and BL formation. We investigated the ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species. In iSCNT embryos, nucleoli precursor bodies originate from the oocyte, while most proteins engaged in the formation of mature nucleoli should be transcribed from genes de novo in the donor nucleus at the time of EGA. Thus, the success of nucleoli formation depends on species compatibility of many components of this complex process. We demonstrate that the time and cell stage of nucleoli formation are under the control of recipient ooplasm. Oocytes of the studied species possess different abilities to support nucleoli formation. Formation of nucleoli, which is a complex but small part of the whole process of EGA, is essential but not absolutely sufficient for the development of iSCNT embryos to the morula and BL stages.

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Section: Discussioncontrasting

confidence: 61%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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“…However, an effect was expressed in a greater incidence of developmental arrest at <8 cells as well as reduced overall percentage of blastocysts and blastomeres per blastocyst. The developmental stage most sensitive to this initial hyperosmotic condition was near the 8-cell stage, an interval associated with activation of the embryonic genome (Hoffert et al, 1997). Exposures to osmolarities ≥1,000 mOsm impacted the embryo development first (50-80% decrease in the proportion of blastocysts) and then nuclear maturation (25% decrease in MII).…”

Section: Discussionmentioning

confidence: 99%

Impact of anisosmotic conditions on structural and functional integrity of cumulus–oocyte complexes at the germinal vesicle stage in the domestic cat

Comizzoli

Wildt

Pukazhenthi

2007

Molecular Reproduction Devel

Self Cite

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During cryopreservation, the immature oocyte is subjected to anisosmotic conditions potentially impairing subsequent nuclear and cytoplasmic maturation in vitro. In preparation for cryopreservation protocols and to characterize osmotic tolerance, cat cumulus-oocyte complexes (COC) at the germinal vesicle (GV) stage were exposed for 15 min to sucrose solutions ranging from 100 to 2,000 mOsm and then examined for structural integrity and developmental competence in vitro. Osmolarities ≥200 and ≤750 mOsm had no effect on incidence of oocyte nuclear maturation, fertilization success, and blastocyst formation compared to control COC (exposed to 290 mOsm). This relatively high osmotic tolerance of the immature cat oocyte appeared to arise from a remarkable stability of the GV chromatin structure as well as plasticity in mitochondrial distribution, membrane integrity, and ability to maintain cumulus-oocyte communications. Osmolarities < 200 mOsm only damaged cumulus cell membrane integrity, which contributed to poor nuclear maturation but ultimately had no adverse effect on blastocyst formation in vitro. Osmolarities >750 mOsm compromised nuclear maturation and blastocyst formation in vitro via disruption of cumulus-oocyte communications, an effect that could be mitigated through 1,500 mOsm by adding cytochalasin B to the hyperosmotic solutions. These results (1) demonstrate, for the first time, the expansive osmotic tolerance of the immature cat oocyte, (2) characterize the fundamental role of cumulus-oocyte communications when tolerance limits are exceeded, and (3) reveal an interesting hyperosmotic tolerance of the immature oocyte that can be increased two-fold by supplementation with cytochalasin B.

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“…The timing of embryonic gene activation is species-specific (Manes, 1973;Camous et al, 1986;Braude et al, 1988;Crosby et al, 1988;Zernicka-Goetz, 1994;Hoffert et al, 1997). In mouse a major burst of zygotic gene expression occurs at the two-cell stage corresponding to the time at which mRNAs for the majority of maternal transcripts are degraded (Schultz, 1993).…”

Section: Introductionmentioning

confidence: 99%

Maternal Argonaute 2 Is Essential for Early Mouse Development at the Maternal-Zygotic Transition

Lykke-Andersen

Gilchrist

Grabarek

et al. 2008

MBoC

107

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Activation of zygotic gene expression in the two-cell mouse embryo is associated with destruction of maternally inherited transcripts, an important process for embryogenesis about which little is understood. We asked whether the Argonaute (Ago)/RNA-induced silencing complex, providing the mRNA "slicer" activity in gene silencing, might contribute to this process. Here we show that Ago2, 3, and 4 transcripts are contributed to the embryo maternally. By systematic knockdown of maternal Ago2, 3, and 4, individually and in combination, we find that only Ago2 is required for development beyond the two-cell stage. Knockdown of Ago2 stabilizes one set of maternal mRNAs and reduces zygotic transcripts of another set of genes. Ago2 is localized in mRNA-degradation P-bodies analogous to those that function in RNAi-like mechanisms in other systems. Profiling the expression of microRNAs throughout preimplantation development identified several candidates that could potentially work with Ago2 to mediate degradation of specific mRNAs. However, their low abundance raises the possibility that other endogenous siRNAs may also participate. Together, our results demonstrate that maternal expression of Ago2 is essential for the earliest stages of mouse embryogenesis and are compatible with the notion that degradation of a proportion of maternal messages involves the RNAi-machinery.

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Transition from maternal to embryonic control of development in IVM/IVF domestic cat embryos

Cited by 46 publications

References 43 publications

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Impact of anisosmotic conditions on structural and functional integrity of cumulus–oocyte complexes at the germinal vesicle stage in the domestic cat

Maternal Argonaute 2 Is Essential for Early Mouse Development at the Maternal-Zygotic Transition

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