The spectral heat content is investigated for time-changed killed Brownian motions on C 1,1 open sets, where the time change is given by either a subordinator or an inverse subordinator, with the underlying Laplace exponent being regularly varying at ∞ with index β ∈ (0, 1). In the case of inverse subordinators, the asymptotic limit of the spectral heat content is shown to involve a probabilistic term depending only on β ∈ (0, 1). In contrast, in the case of subordinators, this universality holds only when β ∈ ( 1 2 , 1).