Transient Switching of NMDA-Dependent Long-Term Synaptic Potentiation in CA3-CA1 Hippocampal Synapses to mGluR1-Dependent Potentiation After Pentylenetetrazole-Induced Acute Seizures in Young Rats

Postnikova, Tatyana Y.; Trofimova, Alina M.; Ergina, Julia L.; Зубарева, О. Е.; Kalemenev, S. V.; Zaitsev, Aleksey V.

doi:10.1007/s10571-018-00647-3

Cited by 17 publications

(14 citation statements)

References 87 publications

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“…In our experiments, LTP induction did not require the activity of NMDARs because it was preserved, even when the NMDARs were blocked with MK-801. This form of plasticity resembles the LTP observed in the hippocampal slices of rats one day after pentylenetetrazole-induced status epilepticus [42].…”

Section: Discussionsupporting

confidence: 66%

“…Their relative roles in plasticity may change in some pathological conditions. For example, recently, we demonstrated a decrease in LTP magnitude due to transient switching between NMDA-dependent and mGluR1-dependent forms of LTP following pentylenetetrazole-induced status epilepticus in young rats [42]. Similar changes may occur after LPS administration.…”

Section: Introductionmentioning

confidence: 62%

“…Its application did not significantly affect LTP magnitude in In the presence of MK-801, the amplitude of fEPSPs in the 3wLPS_y group slowly increased after TBS and reached a maximum after 30-60 min ( Figure 4C). Such a change in the amplitude of fEPSPs after the induction protocol is a characteristic of mGluR-dependent forms of LTP [33,42,56]. Therefore, we tested the effect of a selective mGluR1 antagonist, FTIDC (4-[1-(2-fluoropyridin-3-yl)-5-methyltriazol-4-yl]-N-methylN-propan-2-yl-3,6dihydro-2H-pyridine -1-carboxamide) (5 µM) [57], on LTP induction in different groups.…”

Section: Pharmacological Properties Of Ltp Changed In Young Rats Follmentioning

confidence: 99%

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Administration of Bacterial Lipopolysaccharide during Early Postnatal Ontogenesis Induces Transient Impairment of Long-Term Synaptic Plasticity Associated with Behavioral Abnormalities in Young Rats

et al. 2020

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Infectious diseases in early postnatal ontogenesis often result in cognitive impairments, particularly learning and memory. The essential foundation of learning and memory is long-term synaptic plasticity, which depends on N-methyl-D-aspartate (NMDA) receptors. In the present study, bacterial infection was modeled by treating rat pups with bacterial lipopolysaccharide (LPS, 25 µg/kg) three times, during either the first or the third week of life. These time points are critical for the maturation of NMDA receptors. We assessed the effects of LPS treatments on the properties of long-term potentiation (LTP) in the CA1 hippocampus of young (21-23 days) and adolescent (51-55 days) rats. LTP magnitude was found to be significantly reduced in both groups of young rats, which also exhibited investigative and motor behavior disturbances in the open field test. No changes were observed in the main characteristics of synaptic transmission, although the LTP induction mechanism was disturbed. In rats treated with LPS during the third week, the NMDA-dependent form of LTP was completely suppressed, and LTP switched to the Type 1 metabotropic glutamate receptor (mGluR1)-dependent form. These impairments of synaptic plasticity and behavior were temporary. In adolescent rats, no difference was observed in LTP properties between the control and experimental groups. Lastly, the investigative and motor behavior parameters in both groups of adult rats were similar.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 62%

Section: Pharmacological Properties Of Ltp Changed In Young Rats Follmentioning

confidence: 99%

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Administration of Bacterial Lipopolysaccharide during Early Postnatal Ontogenesis Induces Transient Impairment of Long-Term Synaptic Plasticity Associated with Behavioral Abnormalities in Young Rats

et al. 2020

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“…We then selected the most suitable reference genes for RT-qPCR analysis within different brain areas in the pentylenetetrazole (PTZ) seizure model in immature rats. The PTZ model of seizures is widely used to investigate the mechanism of neuropsychiatric disturbances caused by severe childhood seizures [20][21][22][23][24]. Immature rats are more sensitive to PTZ compared to adults, and PTZ induces prolonged tonic-clonic seizures [25].…”

Section: Ptz Model Of Seizuresmentioning

confidence: 99%

“…Immature rats are more sensitive to PTZ compared to adults, and PTZ induces prolonged tonic-clonic seizures [25]. PTZ-induced seizures are characterized by massive activation of a broad spectrum of cortical and subcortical brain areas, especially in young rats, leading to delayed changes in synaptic plasticity and behavior [21,22,[24][25][26][27]].…”

Section: Ptz Model Of Seizuresmentioning

confidence: 99%

Reference Gene Validation in the Brain Regions of Young Rats after Pentylenetetrazole-Induced Seizures

et al. 2020

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Reverse transcription followed by quantitative polymerase chain reaction (qRT-PCR) is a powerful and commonly used tool for gene expression analysis. It requires the right choice of stably expressed reference genes for accurate normalization. In this work, we aimed to select the optimal reference genes for qRT-PCR normalization within different brain areas during the first week following pentylenetetrazole-induced seizures in immature (P20–22) Wistar rats. We have tested the expression stability of a panel of nine housekeeping genes: Actb, Gapdh, B2m, Rpl13a, Sdha, Ppia, Hprt1, Pgk1, and Ywhaz. Based on geometric averaging of ranks obtained by four common algorithms (geNorm, NormFinder, BestKeeper, Comparative Delta-Ct), we found that the stability of tested reference genes varied significantly between different brain regions. The expression of the tested panel of genes was very stable within the medial prefrontal and temporal cortex, and the dorsal hippocampus. However, within the ventral hippocampus, the entorhinal cortex and amygdala expression levels of most of the tested genes were not steady. The data revealed that in the pentylenetetrazole-induced seizure model in juvenile rats, Pgk1, Ppia, and B2m expression are the most stable within the medial prefrontal cortex; Ppia, Rpl13a, and Sdha within the temporal cortex; Pgk1, Ppia, and Rpl13a within the entorhinal cortex; Gapdh, Ppia, and Pgk1 within the dorsal hippocampus; Rpl13a, Sdha, and Ppia within the ventral hippocampus; and Sdha, Pgk1, and Ppia within the amygdala. Our data indicate the need for a differential selection of reference genes across brain regions, including the dorsal and ventral hippocampus.

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Long-term potentiation enhancing effect of epileptic insult in the CA1 area is dependent on prior-application of primed-burst stimulation

et al. 2020

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Transient Switching of NMDA-Dependent Long-Term Synaptic Potentiation in CA3-CA1 Hippocampal Synapses to mGluR1-Dependent Potentiation After Pentylenetetrazole-Induced Acute Seizures in Young Rats

Cited by 17 publications

References 87 publications

Administration of Bacterial Lipopolysaccharide during Early Postnatal Ontogenesis Induces Transient Impairment of Long-Term Synaptic Plasticity Associated with Behavioral Abnormalities in Young Rats

Administration of Bacterial Lipopolysaccharide during Early Postnatal Ontogenesis Induces Transient Impairment of Long-Term Synaptic Plasticity Associated with Behavioral Abnormalities in Young Rats

Reference Gene Validation in the Brain Regions of Young Rats after Pentylenetetrazole-Induced Seizures

Long-term potentiation enhancing effect of epileptic insult in the CA1 area is dependent on prior-application of primed-burst stimulation

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