Transient Sub-cellular Localization and In Vivo Protein-Protein Interaction Study of Multiple Abiotic Stress-Responsive AteIF4A-III and AtALY4 Proteins in Arabidopsis thaliana

SlHyPRP1 and DEA1, the multiple stress responsive eight-cysteine motif family genes of tomato (Solanum lycopersicum L.) are expressed tissue specifically, localize and interact at cytoplasm and plasma membrane in vivo

“…To validate the in silico protein partners of SlHyPRP1 and DEA1 in vivo, we performed a systematic Split-YFP (Bi-FC) assay (Gehl et al 2009;Baruah et al 2020) using the protoplasts of tomato (cv. Arka Vikas).…”

“…Generally, breeding for tolerance to single stress is not ideal as plants respond distinctively to different or concurrent stresses (Pandey et al 2017). The occurrence of multiple abiotic stresses such as drought, temperature extremes and salinity are known to be the major factors that influence the outburst of a range of biotic stress factors (Chikkaputtaiah et al 2017;Baruah et al 2020). Together these multiple biotic and abiotic stresses severely affect overall plant growth, innate tolerance capacity, productivity and the yield (Debbarma et al 2019).…”