Transient Receptor Potential Vanilloid Type 1 (TRPV1) Expression Changes from Normal Urothelium to Transitional Cell Carcinoma of Human Bladder

Lazzeri, Massimo; Vannucchi, Maria Giuliana; Spinelli, M.; Bizzoco, Elisa; Beneforti, P.; Turini, Damiano; Faussone–Pellegrini, Maria Simonetta

doi:10.1016/j.eururo.2005.05.018

Cited by 104 publications

(90 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TRPV cation channel family shows homeostatic and regulatory functions (calcium influx, apoptosis, and cell proliferation). In addition, recent data indicate that changes in their expression contribute to malignant growth and progression [11,12] and a role for TRPV6 and TRPV1 in the progression of prostate and bladder cancer and gliomas was reported [13][14][15]. At present, no data concerning the TRPV2 channel expression in normal and tumour human bladder tissues are available, and it is still unknown whether changes in TRPV2 expression could be associated with the disease stage in UC.…”

Section: Introductionmentioning

confidence: 93%

“…By immunohistochemistry we showed that in normal bladder tissues, TRPV2 is localised in the superficial cells, whereas negligible or no expression is observed in basal and club-shaped cells. Because changes in TRPV channel expression have been associated with tumour development, growth, and progression [13][14][15], we evaluated whether urothelial cell transformation could be associated with changes in the TRPV2 expression. We found that TRPV2 protein is expressed both in low-grade RT4 and high-grade EJ and J82 cells, with higher expression observed in J82 cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transient Receptor Potential Vanilloid Type 2 (TRPV2) Expression in Normal Urothelium and in Urothelial Carcinoma of Human Bladder: Correlation with the Pathologic Stage

et al. 2008

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Transient Receptor Potential Vanilloid Type 2 (TRPV2) Expression in Normal Urothelium and in Urothelial Carcinoma of Human Bladder: Correlation with the Pathologic Stage

et al. 2008

View full text Add to dashboard Cite

“…All these findings strongly suggest that functional TRPV1 channels may hardly exist in mouse urothelial cells in normal conditions. Several IHC studies have reported that TRPV1 is also expressed in human urothelial cells (Lazzeri et al 2004(Lazzeri et al ,2005Apostolidis et al 2005a,b). However, other studies have cast doubt on those experiments (Dinis et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Differential Localizations of the Transient Receptor Potential Channels TRPV4 and TRPV1 in the Mouse Urinary Bladder

Yamada

Ugawa

Ueda

et al. 2008

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R Y We studied the localization and physiological functions of the transient receptor potential (TRP) channels TRPV1 (TRP vanilloid 1) and TRPV4 (TRP vanilloid 4) in the mouse bladder, because both channels are thought to be mechanosensors for bladder distention. RT-PCR specifically amplified TRPV4 transcripts from the urothelial cells, whereas TRPV1 transcripts were barely detectable. ISH experiments showed that TRPV4 transcripts were abundantly expressed in the urothelium, whereas TRPV1 transcripts were not detectable in the urothelial cells. Immunoblotting and IHC studies showed that TRPV4 proteins were mainly localized at the basal plasma membrane domains of the basal urothelial cells. In contrast, TRPV1-immunoreactivities were found not in the urothelial cells but in the nerve fibers that innervate the urinary bladder. In Ca 21-imaging experiments, 4a-phorbol 12,13-didecanoate, a TRPV4 agonist, and hypotonic stimuli induced significant increases in intracellular calcium ion concentration ([Ca 21 ] i ) in isolated urothelial cells, whereas capsaicin, a TRPV1 agonist, showed no marked effect on the cells. These findings raise the possibility that, in mouse urothelial cells, TRPV4 may contribute to the detection of increases in intravesical pressure related to the micturition reflex. (J Histochem Cytochem 57:277-287, 2009)

show abstract

“…Although the biological role of TRPV1 in cells other than primary sensory neurons is unclear, some studies indicated a role for TRPV1 in carcinogenesis. Increased expression of TRPV1 was detected in prostate, colon, and pancreatic cancers (12), whereas the expression of TRPV1 was found to decrease as bladder cancer and brain tumor malignancy progressed (13,14), suggesting that TRPV1 might negatively control tumor progression. Our recent study also suggested that TRPV1 might act as a tumor suppressor in skin cancer (15).…”

Section: Introductionmentioning

confidence: 99%

Cocarcinogenic Effect of Capsaicin Involves Activation of EGFR Signaling but Not TRPV1

et al. 2010

View full text Add to dashboard Cite

Epidemiologic and animal studies revealed that capsaicin can act as a carcinogen or cocarcinogen. However, the molecular mechanisms of the cancer-promoting effects of capsaicin are not clear. Here, we report that capsaicin has a cocarcinogenic effect on 12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted skin carcinogenesis in vivo and is mediated through the epidermal growth factor receptor (EGFR), but not the transient receptor potential vanilloid subfamily member 1 (TRPV1). Topical application of capsaicin on the dorsal skin of 7,12-dimetylbenz(a)anthracene-initiated and TPA-promoted TRPV1 wild-type (WT) and TRPV1 knockout (KO) mice induced more and larger skin tumors in TRPV1/KO mice, suggesting a TRPV1-independent mechanism. Cyclooxygenase-2 (COX-2) was highly elevated by capsaicin treatment in tumors and murine embryonic fibroblasts from TRPV1/KO mice. Inhibitors of EGFR/MEK signaling suppressed TPA/capsaicininduced COX-2 expression in TRPV1/KO cells, indicating that activation of EGFR and its downstream signaling is involved in COX-2 elevation. Capsaicin induced a further induction of TPA-increased COX-2 expression in EGFR/WT cells, but not in EGFR/KO cells. TPA/capsaicin cotreatment caused EGFR tyrosine phosphorylation and activated EGFR downstream signaling, including ERKs and Akt in EGFR/WT, but not EGFR/KO cells. Specific inhibition of EGFR and TRPV1 indicated that capsaicin-induced ERK activation in A431 cells was dependent on EGFR, but not TRPV1. Together, these findings suggest that capsaicin might act as a cocarcinogen in TPAinduced skin carcinogenesis through EGFR-dependent mechanisms. Cancer Res; 70(17); 6859-69. ©2010 AACR.

show abstract

Transient Receptor Potential Vanilloid Type 1 (TRPV1) Expression Changes from Normal Urothelium to Transitional Cell Carcinoma of Human Bladder

Cited by 104 publications

References 34 publications

Transient Receptor Potential Vanilloid Type 2 (TRPV2) Expression in Normal Urothelium and in Urothelial Carcinoma of Human Bladder: Correlation with the Pathologic Stage

Transient Receptor Potential Vanilloid Type 2 (TRPV2) Expression in Normal Urothelium and in Urothelial Carcinoma of Human Bladder: Correlation with the Pathologic Stage

Differential Localizations of the Transient Receptor Potential Channels TRPV4 and TRPV1 in the Mouse Urinary Bladder

Cocarcinogenic Effect of Capsaicin Involves Activation of EGFR Signaling but Not TRPV1

Contact Info

Product

Resources

About