Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Mihara, Hiroshi; Suzuki, Nobuhiro; Boudaka, Ammar; Muhammad, Jibran Sualeh; Tominaga, Makoto; Tabuchi, Yoshiaki; Sugiyama, Toshiro

doi:10.3748/wjg.v22.i24.5512

Cited by 26 publications

(32 citation statements)

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“…Given that TRPV4 was shown to be expressed in the esophagus and colon epithelium, and that we recently showed that TRPV4 is expressed in mouse and rat gastric epithelia and is suppressed in several cancers, we examined TRPV4 mRNA expression in human gastric biopsy samples as well as AGS cells . TRPV4 mRNA was detected in HP (‐) normal human gastric biopsy samples, but not in AGS cells (Figure A) or in several other gastric cancer cell lines (eg, MKN28, MKN45, data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…We previously reported that TRPV4 is expressed in mouse and rat gastric epithelium where it contributes to ATP release and gastric emptying . Current data suggested that HP‐infected individuals experience abnormal detection of gastric pressure and gastric sensory or motor dysfunction.…”

Section: Discussionmentioning

confidence: 99%

“…ATP concentrations released from AGS cells cultured in 12‐well plates were measured by a luciferin‐luciferase assay (ATP Bioluminescence assay kit CLS II, Roche Diagnostics) and a luminometer (Lumat LB 9507, Berthold Technologies, Japan), using a previously described method . For chemical stimuli, cells cultured to 70%‐80% confluence and incubated in 500 μL bath solution for 30 minutes at room temperature (25°C) were used to measure basal ATP release.…”

Section: Methodsmentioning

confidence: 99%

“…TRPV4 is widely expressed throughout the gastrointestinal epithelium, including the esophagus, intestine, and gastric epithelium where it contributes to adenosine triphosphate (ATP) release via exocytosis . We recently reported that TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying . However, to the best of our knowledge, to date, there are no reports on TRPV4 expression analysis in human gastric epithelium.…”

Section: Introductionmentioning

confidence: 99%

“…10,11 We recently reported that TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying. 12 However, to the best of our knowledge, to date, there are no reports on TRPV4 expression analysis in human gastric epithelium.…”

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confidence: 99%

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Transient receptor potential vanilloid 4 (TRPV4) silencing in Helicobacter pylori‐infected human gastric epithelium

et al. 2016

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Background Helicobacter pylori (HP) infection induces methylation silencing of specific genes in gastric epithelium. Various stimuli activate the nonselective cation channel TRPV4, which is expressed in gastric epithelium where it detects mechanical stimuli and promotes ATP release. As CpG islands in TRPV4 are methylated in HP‐infected gastric epithelium, we evaluated HP infection‐dependent changes in TRPV4 expression in gastric epithelium.Materials and MethodsHuman gastric biopsy samples, a human gastric cancer cell line (AGS), and a normal gastric epithelial cell line (GES‐1) were used to detect TRPV4 mRNA and protein expression by RT‐PCR and Western blotting, respectively. Ca2+ imaging was used to evaluate TRPV4 ion channel activity. TRPV4 methylation status was assessed by methylation‐specific PCR (MSP). ATP release was measured by a luciferin‐luciferase assay.Results TRPV4 mRNA and protein were detected in human gastric biopsy samples and in GES‐1 cells. MSP and demethylation assays showed TRPV4 methylation silencing in AGS cells. HP coculture directly induced methylation silencing of TRPV4 in GES‐1 cells. In human samples, HP infection was associated with TRPV4 methylation silencing that recovered after HP eradication in a time‐dependent manner.Conclusion HP infection‐dependent DNA methylation suppressed TRPV4 expression in human gastric epithelia, suggesting that TRPV4 methylation may be involved in HP‐associated dyspepsia.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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