Transient Receptor Potential Is Essential for High Temperature Tolerance in Invasive Bemisia tabaci Middle East Asia Minor 1 Cryptic Species

Abstract

Temperature is an important factor in affecting population dynamics and diffusion distribution of organisms. Alien species can successfully invade and colonize to various temperature environments, and one of important reasons is that alien species have a strong resistance to stress temperature. Recently, researchers have focused on the mechanisms of temperature sensing to determine the sensing and regulation mechanisms of temperature adaptation. The transient receptor potential (TRP) is one of the key components of an organism’s temperature perception system. TRP plays important roles in perceiving temperature, such as avoiding high temperature, low temperature and choosing the optimum temperature. To assess high temperature sensation and the heat resistance role of the TRP gene, we used 3′ and 5′ rapid-amplification of cDNA ends to isolate the full-length cDNA sequence of the TRP gene from Bemisia tabaci (Gennadius) MEAM1 (Middle East Asia Minor 1), examined the mRNA expression profile under various temperature conditions, and identified the heat tolerance function. This is the first study to characterize the TRP gene of invasive B. tabaci MEAM1 (MEAM1 BtTRP). The full-length cDNA of MEAM1 BtTRP was 3871 bp, and the open reading frames of BtTRP was 3501 bp, encoding 1166 amino acids. Additionally, the BtTRP mRNA expression level was significantly increased at 35°C. Furthermore, compared with control treatments, the survival rate of B. tabaci MEAM1 adults was significantly decreased under high temperature stress conditions after feeding with dsRNA BtTRP. Collectively, these results showed that MEAM1 BtTRP is a key element in sensing high temperature and plays an essential role in B. tabaci MEAM1 heat tolerance ability. Our data improved our understanding of the mechanism of temperature sensation in B. tabaci MEAM1 at the molecular level and could contribute to the understanding of the thermal biology of B. tabaci MEAM1 within the context of global climate change.

Selection and validation of reference genes for qRT-PCR analysis during biological invasions: The thermal adaptability of Bemisia tabaci MED

“…B . tabaci has emerged as a promising model to understand the molecular basis of abiotic stress tolerance and adaptation for invasion biology [ 34 – 37 ]. As an invasive species, thermotolerance represent crucial mechanism for MED to successfully colonize new habitats [ 38 – 40 ].…”

Molecular Characterization of TRPA Subfamily Genes and Function in Temperature Preference in Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

“…In previous studies, it was believed that the TRP channel usually contains four subunits, each of which has six transmembrane proteins, and its N-terminal and C-terminal are both intracellular [58], which is consistent with the protein prediction results of the TaPyx protein. However, studies in invertebrates have also found that the N-terminal and C-terminal of the TRP channel exist extracellular, such as the TRP channel in B. tabaci [51]. In fact, TRP is a class of nonselective cationic channel proteins that exist on the membrane of the cell membrane or intracellular organelle membrane, and the mechanism of its signal conversion in many systems needs to be further studied.…”

“…In the study of two cryptic species of Bemisia tabaci AsiaII3 and MEAM1 (Middle East Asia Minor 1), it was found that temperature stress could induce the expression of BtTRP mRNA, and played an important role in heat tolerance. According to this characteristic, researchers infer that BtTRP also belongs to the TRPA subfamily [51][52][53]. Therefore, the TRPA subfamily plays an important role in temperature sensing and the heat escape injury response.…”

Characterization of Chromatin Remodeling Genes Involved in Thermal Tolerance of Biologically Invasive Bemisia tabaci

“…Previous studies have shown that exposure of invasive whiteflies to thermal stress is accompanied by rapid alterations in their gene expression ( Lü and Wan, 2008 ; Mahadav et al, 2009 ; Lü et al, 2014a ; Dai et al, 2018a ). Lü et al (2014b) conducted heat shock experiments with MED and found that its survival rate was significantly improved within two generations.…”