Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

Chen, Si‐Chong; Takahashi, Nobuaki; Chen, Changping; Pauli, Jordan L; Kuroki, Chiharu; Kaminosono, Jun; Kashiwadani, Hideki; Kanmura, Yuichi; Mori, Yasuo; Ou, Shaowu; Liang, Hao; Kuwaki, Tomoyuki

doi:10.3389/fphys.2020.576209

Cited by 9 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the attenuated genes in SCI mice administered GBP, Trpa1 encodes the transient receptor potential ankyrin 1 (TRPA1), a non-selective cation channel expressed in sensory pathways. TRPA1 is activated during hypoxia (Chen et al, 2020 ) and activation of Trpa1-positive sensory pathways has been shown to induce hypothermia (Matsuo et al, 2021 ). Fgf13 encodes the fibroblast growth factor 13 (FGF13), a member of the FGF family that is involved in a variety of biological processes including development, cell growth, morphogenesis and invasion.…”

Section: Resultsmentioning

confidence: 99%

Breaking Mental Barriers Promotes Recovery After Spinal Cord Injury

Rodocker

Bordbar

Larson

et al. 2022

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Functional recovery after spinal cord injury (SCI) often proves difficult as physical and mental barriers bar survivors from enacting their designated rehabilitation programs. We recently demonstrated that adult mice administered gabapentinoids, clinically approved drugs prescribed to mitigate chronic neuropathic pain, recovered upper extremity function following cervical SCI. Given that rehabilitative training enhances neuronal plasticity and promotes motor recovery, we hypothesized that the combination of an aerobic-based rehabilitation regimen like treadmill training with gabapentin (GBP) administration will maximize recovery in SCI mice by strengthening synaptic connections along the sensorimotor axis. Whereas mice administered GBP recovered forelimb functions over the course of weeks and months following SCI, no additive forelimb recovery as the result of voluntary treadmill training was noted in these mice. To our surprise, we also failed to find an additive effect in mice administered vehicle. As motivation is crucial in rehabilitation interventions, we scored active engagement toward the rehabilitation protocol and found that mice administered GBP were consistently participating in the rehabilitation program. In contrast, mice administered vehicle exhibited a steep decline in participation, especially at chronic time points. Whereas neuroinflammatory gene expression profiles were comparable between experimental conditions, we discovered that mice administered GBP had increased hippocampal neurogenesis and exhibited less anxiety-like behavior after SCI. We also found that an external, social motivator effectively rescues participation in mice administered vehicle and promotes forelimb recovery after chronic SCI. Thus, not only does a clinically relevant treatment strategy preclude the deterioration of mental health after chronic SCI, but group intervention strategies may prove to be physically and emotionally beneficial for SCI individuals.

show abstract

Section: Resultsmentioning

confidence: 99%

Breaking Mental Barriers Promotes Recovery After Spinal Cord Injury

Rodocker

Bordbar

Larson

et al. 2022

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…A positron emission tomography study shows that the condition of acute hunger for air after inhalation of carbon dioxide activate the ACC region 21 . An animal study has also demonstrated that TRPA1 is involved in hypoxia-induced behaviors in mice 37 . For instance, TRPA1 knockout (KO) mice have attenuated avoidance behaviors in response to a low O 2 environment and ventilator responses to mild hypoxia.…”

Section: Discussionmentioning

confidence: 97%

TRPA1 as a O2 sensor detects microenvironmental hypoxia in the mice anterior cingulate cortex

Kawabata

Shimoyama

Ueno

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Transient receptor potential ankyrin 1 (TRPA1) is a member of the TRP channel family and is expressed in peripheral and central nervous systems. In the periphery, TRPA1 senses cold and pain. However, the functions of TRPA1 in the CNS are unclear. Here, we examined the roles of TRPA1 on neural activity and synaptic transmission in layer II/III pyramidal neurons from mice anterior cingulate cortex (ACC) by whole-cell patch-clamp recordings. The activation of Cinnamaldehyde (CA), which is TRPA1 agonist produced inward currents and these were blocked by the TRPA1 antagonists. Furthermore, activating TRPA1 changed the properties of action potentials such as the firing rate, rise time and decay time. In contrast, stimulating TRPA1 did not alter the spontaneous synaptic transmission. Finally, we examined the functional role of TRPA1 on neurons in a hypoxic environment. We induced an acute hypoxia by substituting nitrogen (N2) gas for oxygen (O2) in the external solution. N2 produced biphasic effects that consisting of inward currents in the early phase and outward currents in the late phase. Importantly, blocking TRPA1 reduced inward currents, but not outward currents. In contrast, a KATP channel blocker completely inhibited outward currents. These results suggest that TRPA1 acts on postsynaptic neurons in the ACC as an acute O2 sensor.

show abstract

“…A positron emission tomography study shows that the condition of acute hunger for air after inhalation of carbon dioxide activate the ACC region 20 . An animal study has also demonstrated that TRPA1 is involved in hypoxia-induced behaviors in mice 32 . For instance, TRPA1 knockout (KO) mice have attenuated avoidance behaviors in response to a low O 2 environment and ventilator responses to mild hypoxia.…”

Section: Discussionmentioning

confidence: 97%

TRPA1 as a O2 sensor detects microenvironmental hypoxia in the mice anterior cingulate cortex

Kawabata

Yao

Azuma

et al. 2022

Preprint

View full text Add to dashboard Cite

Transient receptor potential ankyrin 1 (TRPA1) is a member of the TRP channel family and is expressed in peripheral and central nervous systems. In the periphery, TRPA1 senses cold and pain. However, the functions of TRPA1 in the CNS are unclear. Here, we examined the roles of TRPA1 on neural activity and synaptic transmission in layer II/III pyramidal neurons from mice anterior cingulate cortex (ACC) by whole-cell patch-clamp recordings. The activation of TRPA1 produced inward currents that were blocked by the TRPA1 antagonist. Furthermore, activating TRPA1 changed the properties of action potentials such as the frequency, rise time and decay time. In contrast, stimulating TRPA1 did not alter the spontaneous synaptic transmission. Finally, we examined the functional role of TRPA1 on neurons in a hypoxic environment. We induced an acute hypoxia by substituting nitrogen (N2) gas for oxygen (O2) in the external solution. N2 produced biphasic effects that consisting of inward currents in the early phase and outward currents in the late phase. Importantly, blocking TRPA1 reduced inward currents, but not outward currents. In contrast, a KATP channel blocker completely inhibited outward currents. These results suggest that TRPA1 acts on postsynaptic neurons in the ACC as an acute O2 sensor.

show abstract

Transient Receptor Potential Ankyrin 1 Mediates Hypoxic Responses in Mice

Cited by 9 publications

References 27 publications

Breaking Mental Barriers Promotes Recovery After Spinal Cord Injury

Breaking Mental Barriers Promotes Recovery After Spinal Cord Injury

TRPA1 as a O2 sensor detects microenvironmental hypoxia in the mice anterior cingulate cortex

TRPA1 as a O2 sensor detects microenvironmental hypoxia in the mice anterior cingulate cortex

Contact Info

Product

Resources

About