Transient Receptor Potential Ankyrin 1 Is Expressed by Inhibitory Motoneurons of the Mouse Intestine

Poole, Daniel P.; Pelayo, Juan C.; Cattaruzza, Fiore; Kuo, Yien–Ming; Gai, Gregory; Chiu, Jonathon V.; Bron, Romke; Furness, John B.; Grady, Eileen F.; Bunnett, Nigel W.

doi:10.1053/j.gastro.2011.04.049

Cited by 78 publications

(88 citation statements)

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“…Furthermore, a subtype of enteroendocrine cells has been shown to express abundant TRPA1 whose stimulation induces 5-HT release that can activate intrinsic nerves and vagal endings (39). These reports strongly suggest that TRPA1 may play a role in the regulation of gut motility as confirmed by several motility studies of experimental animals using TRPA1 ligands and gene-manipulation (11,12,26,42,43). More recently, considerable attention has been given to the presence of TRPA1 in IE cells.…”

Section: Discussionmentioning

confidence: 86%

“…The distribution of TRPA1 appears related to its physiological and pathophysiological roles such as mechanosensation (6, 9, 59), chemosensation (9) and inflammatory hyperalgesia (7,36,57). In addition, a recent study has also verified the presence of TRPA1 in several types of enteric nerves including inhibitory motoneurons, descending interneurons, and intrinsic primary afferent neurons (43). Furthermore, a subtype of enteroendocrine cells has been shown to express abundant TRPA1 whose stimulation induces 5-HT release that can activate intrinsic nerves and vagal endings (39).…”

Section: Discussionmentioning

confidence: 99%

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Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine

Kono

Kaneko

Omiya

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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. TU-100 contains multiple TRP activators. In the present study, therefore, we examined the involvement of TRP channels in the ADM-mediated vasodilatatory effect of TU-100. Rats were treated intraduodenally with the TRP vanilloid type 1 (TRPV1) agonist capsaicin (CAP), the TRP ankyrin 1 (TRPA1) agonist allyl-isothiocyanate (AITC), or TU-100, and jejunum IBF was evaluated using laser-Doppler blood flowmetry. All three compounds resulted in vasodilatation, and the vasodilatory effect of TU-100 was abolished by a TRPA1 antagonist but not by a TRPV1 antagonist. Vasodilatation induced by AITC and TU-100 was abrogated by anti-ADM antibody treatment. RT-PCR and flow cytometry revealed that an IEC-6 cell line originated from the small intestine and purified IE cells expressed ADM and TRPA1 but not TRPV1. AITC increased ADM release in IEC cells remarkably, while CAP had no effect. TU-100 and its ingredient 6-shogaol (6SG) increased ADM release dose-dependently, and the effects were abrogated by a TRPA1 antagonist. 6SG showed similar TRPA1-dependent vasodilatation in vivo. These results indicate that TRPA1 in IE cells may play an important role in controlling bowel microcirculation via ADM release. Epithelial TRPA1 appears to be a promising target for the development of novel strategies for the treatment of various gastrointestinal disorders.daikenchuto; TU-100; vasodilatation; 6-shogaol; inflammatory bowel diseases TRANSIENT RECEPTOR POTENTIAL (TRP) channels are nonselective calcium ion channels ubiquitously expressed in many tissues and are known to participate in a broad range of physical, chemical, and environmental stimuli such as taste, temperature, changes in osmolarity, pressure, stretch, and light.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine

Kono

Kaneko

Omiya

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…In human and rat duodenum, TRPA1 was found in enterochromaffin cells by in situ hybridization (ISH) (38). Most recently, Poole et al showed TRPA1 protein expression in colonic enterocytes and myenteric plexus in mice using immunohistochemistry (IHC) (43). Our own recent study suggests the involvement of TRPA1 in the colonic secretory response induced by luminal thymol, an herb extract (25).…”

mentioning

confidence: 84%

Activation of TRPA1 by luminal stimuli induces EP₄-mediated anion secretion in human and rat colon

Kaji

Yasuoka

Karaki

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Kaji I, Yasuoka Y, Karaki S, Kuwahara A. Activation of TRPA1 by luminal stimuli induces EP4-mediated anion secretion in human and rat colon. Am J Physiol Gastrointest Liver Physiol 302: G690-G701, 2012. First published December 29, 2011; doi:10.1152/ajpgi.00289.2011 physiology, anion and fluid secretion is an important function for host defense and is induced by changes in the luminal environment. The transient receptor potential A1 (TRPA1) channel is considered to be a chemosensor in several sensory tissues. Although the function of TRPA1 has been studied in GI motility, its contribution to the transepithelial ion transport system has rarely been discussed. In the present study, we investigated the secretory effect of the potential TRPA1 agonist allyl isothiocyanate (AITC) in rat and human colon using an Ussing chamber. The mucosal application of AITC (10 Ϫ6 -10 Ϫ3 M) induced Cl Ϫ and HCO 3 Ϫ secretion in a concentrationdependent manner, whereas the serosal application induced a significantly weaker effect. AITC-evoked anion secretion was attenuated by tissue pretreatment with piroxicam and prostaglandin (PG) E2; however, this secretion was not affected by TTX, atropine, or extracellular Ca 2ϩ depletion. These experiments indicate that TRPA1 activation induces anion secretion through PG synthesis, independent of neural pathways in the colon. Further analysis also indicates that AITCevoked anion secretion is mediated mainly by the EP4 receptor subtype. The magnitude of the secretory response exhibited segmental heterogeneity in rat colon. Real-time PCR analysis showed the segmental difference was corresponding to the differential expression of EP4 receptor and cyclooxygenase-1 and -2. In addition, RT-PCR, in situ hybridization, and immunohistochemical studies showed TRPA1 expression in the colonic epithelia. Therefore, we conclude that the activation of TRPA1 in colonic epithelial cells is likely involved in the host defense mechanism through rapid anion secretion. colonic chemosensing; prostaglandin E2 THE EPITHELIUM OF THE GASTROINTESTINAL (GI) tract functions not only as a gate for water and nutrients but as a barrier between the luminal and internal environments. Recent studies have suggested that luminal sensory molecules in the small intestine influence nutrient metabolism and local physiological responses (1, 31, 32). Molecular identification of sensory receptors, such as olfactory, taste, and thermo-receptors, which are part of the transient receptor potential (TRP) family, has helped to elucidate chemical-sensing systems that detect the luminal state. However, the physiological roles of these receptors in colonic ion transport have rarely been investigated at the organ level. In particular, transepithelial anion secretion followed by fluid secretion is regulated by diverse systems, including the nervous, endocrine, and immune systems (4, 17), and is considered an important function for host defense. Therefore, experiments at the tissue or organ levels are important for understanding the contribution of ...

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“…15,16 Recently, TRPA1 expression was also observed in colonic myenteric neurons, where it is believed to modulate spontaneous colonic functions. 17 Although TRPA1 has been characterized and studied mainly in the nervous system, its expression was also reported in non-neuronal tissues such as skeletal muscle, lung, small intestine, colon and pancreas. 18 TRPA1 is activated by a wide spectrum of chemical and mechanical stimuli; TRPA1 is known to respond to dietary irritants such as isothyocyanates (mustard oil, wasabi, horseradish) and allycin (garlic), to name only a few.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%

The role of TRPA1 in visceral inflammation and pain

Lapointe

Altier

2011

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Transient Receptor Potential Ankyrin 1 Is Expressed by Inhibitory Motoneurons of the Mouse Intestine

Cited by 78 publications

References 39 publications

Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine

Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine

Activation of TRPA1 by luminal stimuli induces EP₄-mediated anion secretion in human and rat colon

The role of TRPA1 in visceral inflammation and pain

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