Transient poration and cell surface receptor removal from human lymphocytes in vitro by 1 MHz ultrasound

Brayman, Andrew A.; Coppage, Myra; Vaidya, Smita; Miller, Morton W.

doi:10.1016/s0301-5629(99)00039-3

Cited by 70 publications

(47 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11,12,20 This is believed to be due to the physical or thermal effects induced by US application. 8,9 In our studies, no significant increase in temperature was detected (o1.51C) even when using 30 min of TUS.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies also suggested that TUS might enhance gene transfection by accelerating DNA escape from endosomes, alteration of intracellular trafficking, upregulation of genes, protein translation, 5,8 or altering cell membrane permeability, thus creating pores. 11,12 Understanding the mechanism by which TUS delivers genes to cells, its bioeffects on cell membrane, the kinetics of gene entrance to the nucleus and its time of expression are crucial in order to improve transfection efficiency and allow better control of this modality. 13,14 Recently, Optison, an US contrast agents (USCA), has been used to enhance gene delivery when using TUS in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Therapeutic ultrasound-mediated DNA to cell and nucleus: bioeffects revealed by confocal and atomic force microscopy

et al. 2005

View full text Add to dashboard Cite

Therapeutic ultrasound (TUS) has the potential of becoming a powerful nonviral method for the delivery of genes into cells and tissues. Understanding the mechanism by which TUS delivers genes, its bioeffects on cells and the kinetic of gene entrances to the nucleus can improve transfection efficiency and allow better control of this modality when bringing it to clinical settings. In the present study, direct evidence for the role and possible mechanism of TUS (with or without Optison) in the in vitro gene-delivery process are presented. Appling a 1 MHz TUS, at 2 W/cm 2 , 30%DC for 30 min was found to achieve the highest transfection level and efficiency while maintaining high cell viability (480%). Adding Optison further increase transfection level and efficiency by 1.5 to three-fold. Confocal microscopy studies indicate that long-term TUS application localizes the DNA in cell and nucleus regardless of Optison addition. Thus, TUS significantly affects transfection efficiency and protein kinetic expression. Using innovative direct microscopy approaches: atomic force microscopy, we demonstrate that TUS exerts bioeffects, which differ from the ones obtained when Optison is used together with TUS. Our data suggest that TUS alone affect the cell membrane in a different mechanism than when Optison is used.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Therapeutic ultrasound-mediated DNA to cell and nucleus: bioeffects revealed by confocal and atomic force microscopy

et al. 2005

View full text Add to dashboard Cite

show abstract

“…These concerns were raised by studies documenting hemolysis of erythrocytes in vitro in cell suspensions that contained contrast agent and in vivo in mice injected with intravenous contrast agent that were exposed to pulsed ultrasound (Williams et al, 1991;Dalecki et al, 1997b;Miller et al, 1997;Miller and Gies, 1998a,b;Poliachik et al, 1999;Brayman and Miller, 1999). In vitro studies have reported damage to monolayers of cultured cells whose culture media contained contrast agent and were exposed to pulsed ultrasound (Brayman et al, 1999a;Ward et al, 1999;Miller and Bao, 1998;Miller and Quddus, 2000a). Hemorrhage in the vascular beds of the intestine and skin (Miller and Quddus, 2000b;Miller and Gies, 2000) and damage to cells in the heart (Skyba et al, 1998) were also demonstrated in mice and dogs, respectively, following intravenous injection of contrast agent and exposure to pulsed ultrasound.…”

Section: Cavitation With Injected Microbubblesmentioning

confidence: 99%

Ultrasound–biophysics mechanisms

O’Brien

2007

Progress in Biophysics and Molecular Biology

590

416

View full text Add to dashboard Cite

Ultrasonic biophysics is the study of mechanisms responsible for how ultrasound and biological materials interact. Ultrasound-induced bioeffect or risk studies focus on issues related to the effects of ultrasound on biological materials. On the other hand, when biological materials affect the ultrasonic wave, this can be viewed as the basis for diagnostic ultrasound. Thus, an understanding of the interaction of ultrasound with tissue provides the scientific basis for image production and risk assessment. Relative to the bioeffect or risk studies, that is, the biophysical mechanisms by which ultrasound affects biological materials, ultrasound-induced bioeffects are generally separated into thermal and nonthermal mechanisms. Ultrasonic dosimetry is concerned with the quantitative determination of ultrasonic energy interaction with biological materials. Whenever ultrasonic energy is propagated into an attenuating material such as tissue, the amplitude of the wave decreases with distance. This attenuation is due to either absorption or scattering. Absorption is a mechanism that represents that portion of ultrasonic wave that is converted into heat, and scattering can be thought of as that portion of the wave, which changes direction. Because the medium can absorb energy to produce heat, a temperature rise may occur as long as the rate of heat production is greater than the rate of heat removal. Current interest with thermally mediated ultrasound-induced bioeffects has focused on the thermal isoeffect concept. The non-thermal mechanism that has received the most attention is acoustically generated cavitation wherein ultrasonic energy by cavitation bubbles is concentrated. Acoustic cavitation, in a broad sense, refers to ultrasonically induced bubble activity occurring in a biological material that contains pre-existing gaseous inclusions. Cavitation-related mechanisms include radiation force, microstreaming, shock waves, free radicals, microjets and strain. It is more challenging to deduce the causes of mechanical effects in tissues that do not contain gas bodies. These ultrasonic biophysics mechanisms will be discussed in the context of diagnostic ultrasound exposure risk concerns.

show abstract

“…Recent evidence suggests that ultrasound exposure (USE) can increase the permeability of eukaryotic cell membranes to large molecules such as high molecular weight dextrans and plasmid DNA. [8][9][10][11][12] USE also accelerates the membrane-destabilising hexagonal phase transition of dioleoyl phosphatidylcholine (DOPE), a expression after naked DNA transfections. This approach also enhances by four-fold the efficiency of polyplex transfection, yielding transgene expression levels approximately 3000-fold higher than after naked DNA alone.…”

mentioning

confidence: 99%

Microbubble-enhanced ultrasound for vascular gene delivery

et al. 2000

View full text Add to dashboard Cite

Progress in cardiovascular gene therapy has been hampered by concerns over the safety and practicality of viral vectors and the inefficiency of current nonviral transfection techniques. We have previously reported that ultrasound exposure (USE) enhances transgene expression in vascular cells by up to 10-fold after naked DNA transfection, and enhances lipofection by up to three-fold. We report here that performing USE in the presence of microbubble echocontrast agents enhances acoustic cavitation and is associated with approximately 300-fold increments in transgene

show abstract

Transient poration and cell surface receptor removal from human lymphocytes in vitro by 1 MHz ultrasound

Cited by 70 publications

References 23 publications

Therapeutic ultrasound-mediated DNA to cell and nucleus: bioeffects revealed by confocal and atomic force microscopy

Therapeutic ultrasound-mediated DNA to cell and nucleus: bioeffects revealed by confocal and atomic force microscopy

Ultrasound–biophysics mechanisms

Microbubble-enhanced ultrasound for vascular gene delivery

Contact Info

Product

Resources

About