Transient opening of tricellular vertices controls paracellular transport through the follicle epithelium during<i>Drosophila</i>oogenesis

Isasti-Sanchez, Jone; Münz-Zeise, Fenja; Luschnig, Stefan

doi:10.1101/2020.02.29.971168

Cited by 5 publications

(8 citation statements)

References 58 publications

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“…25 In contrast, most ectopic Col IV-GFP in Khc-73 cells is below the zonulae adherentes (ZAs), which demarcate lateral and apical domains (Figures 2A and 2B); there are not occluding junctions present while the BM is being made. [37][38][39] During the developmental stages when Col IV synthesis is high, ectopic extracellular Col IV-GFP accumulates all along the lateral domain in Khc-73 cells (Figures 2C-2E). As Col IV secretion ends, the ectopic Col IV becomes biased to an apical (upper) region of the lateral domain (Figure 2A), likely due to the movement of fibrils onto the BM that clears the basal (lower) regions of the lateral domain (Figure 2F).…”

Section: Khc-73 Biases Bm Protein Secretion To Basal Regionsmentioning

confidence: 99%

Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells

Zajac

Horne‐Badovinac

2022

Current Biology

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Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells Highlights d A kinesin-3 and a kinesin-1 are required for normal BM assembly d Kinesins move Rab10+ BM vesicles on microtubules polarized along two axes d Transport biases BM exocytosis to basal subregions of the basolateral membrane d Loss of kinesins creates ectopic BM networks that disrupt tissue architecture

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Section: Khc-73 Biases Bm Protein Secretion To Basal Regionsmentioning

confidence: 99%

Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells

Zajac

Horne‐Badovinac

2022

Current Biology

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“…Prior studies indicate the presence of electron dense material between FE cells as early as stage 6 ( Müller 2000 ), with a ladder-like appearance of extracellular septa by stage 10B ( Mahowald 1972 ). A recent study of the occluding function in the FE shows a similar pattern of protein localization for endogenously tagged Neuroglian-YFP and Lachesin-GFP, and demonstrates that an intact occluding junction is formed during stage 11 of oogenesis ( Isasti-Sanchez et al 2021 ). It is interesting to note the FE is a secondary epithelium initiated by a mesenchymal to epithelial transition ( Tepass et al 2001 ), and yet SJ biogenesis appears to be very similar to that observed in the primary epithelia found in the embryo.…”

Section: Discussionmentioning

confidence: 78%

Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila

Alhadyian

Shoaib

Ward

2021

G3 Genes|Genomes|Genetics

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Protein components of the invertebrate occluding junction—known as the septate junction (SJ) - are required for morphogenetic developmental events during embryogenesis in Drosophila melanogaster. In order to determine whether SJ proteins are similarly required for morphogenesis during other developmental stages, we investigated the localization and requirement of four representative SJ proteins during oogenesis: Contactin, Macroglobulin complement-related, Neurexin IV, and Coracle. A number of morphogenetic processes occur during oogenesis, including egg elongation, formation of dorsal appendages, and border cell migration. We found that all four SJ proteins are expressed in egg chambers throughout oogenesis, with the highest and most sustained levels in the follicular epithelium (FE). In the FE, SJ proteins localize along the lateral membrane during early and mid-oogenesis, but become enriched in an apical-lateral domain (the presumptive SJ) by stage 10B. SJ protein relocalization requires the expression of other SJ proteins, as well as Rab5 and Rab11 in a manner similar to SJ biogenesis in the embryo. Knocking down the expression of these SJ proteins in follicle cells throughout oogenesis results in egg elongation defects and abnormal dorsal appendages. Similarly, reducing the expression of SJ genes in the border cell cluster results in border cell migration defects. Together, these results demonstrate an essential requirement for SJ genes in morphogenesis during oogenesis, and suggests that SJ proteins may have conserved functions in epithelial morphogenesis across developmental stages. Article Summary: Septate junction (SJ) proteins are essential for forming an occluding junction in epithelial tissues in Drosophila melanogaster, and also for morphogenetic events that occur prior to the formation of the junction during embryogenesis. Here we show that SJ proteins are expressed in the follicular epithelium of egg chambers during oogenesis and are required for morphogenetic events including egg elongation, dorsal appendages formation, and border cell migration. Additionally, the formation of SJs during oogenesis is similar to that in embryonic epithelia.

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“…Prior studies indicate the presence of electron dense material between FE cells as early as stage 6 (Müller 2000), with a ladder-like appearance of extracellular septae by stage 10B (Mahowald 1972). A recent study of the occluding function in the FE show a similar pattern of protein localization for endogenously tagged Neuroglian-YFP and Lachesin-GFP, and demonstrates that an intact occluding junction is formed during stage 11 of oogenesis (Isasti-Sanchez et al 2020). It is interesting to note the FE is a secondary epithelium initiated by a mesenchymal to epithelial transition (Tepass et al 2001), and yet SJ biogenesis appears to be very similar to that observed in the primary epithelia found in the embryo.…”

Section: Biogenesis Of the Sj In The Fementioning

confidence: 86%

Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila

Alhadyian

Shoaib

Ward

2020

Preprint

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Protein components of the invertebrate occluding junction - known as the septate junction (SJ) - are required for morphogenetic developmental events during embryogenesis in Drosophila melanogaster. In order to determine whether SJ proteins are similarly required for morphogenesis during other developmental stages, we investigated the localization and requirement of four representative SJ proteins during oogenesis: Contactin, Macroglobulin complement-related, Neurexin IV, and Coracle. A number of morphogenetic processes occur during oogenesis, including egg elongation, formation of dorsal appendages, and border cell migration. We found that all four SJ proteins are expressed in the egg throughout oogenesis, with the highest and most sustained levels in the follicular epithelium (FE). In the FE, SJ proteins localize along the lateral membrane during early and mid-oogenesis, but become enriched in an apical-lateral domain (the presumptive SJ) by stage 10b. SJ protein relocalization requires the expression of other SJ proteins, as well as rab5 and rab11 in a manner similar to SJ biogenesis in the embryo. Knocking down the expression of these SJ proteins in follicle cells throughout oogenesis results in egg elongation defects and abnormal dorsal appendages. Similarly, reducing the expression of SJ genes in the border cell cluster results in border cell migration defects. Together, these results demonstrate an essential requirement for SJ genes in morphogenesis during oogenesis, and suggests that SJ proteins may have conserved functions in epithelial morphogenesis across developmental stages.

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Transient opening of tricellular vertices controls paracellular transport through the follicle epithelium duringDrosophilaoogenesis

Cited by 5 publications

References 58 publications

Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells

Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells

Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila

Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila

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