Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line

Vachon, Pierre H.; Beaulieu, Jean‐François

doi:10.1016/0016-5085(92)90829-n

Cited by 200 publications

(204 citation statements)

References 39 publications

Supporting

Mentioning

192

Contrasting

Unclassified

Order By: Relevance

“…These cells are unique in that upon reaching confluence they spontaneously undergo a gradual enterocytic differentiation process, similar to that observed in the epithelium of the intact fetal small and large intestine (42)(43)(44)(45). Cells between passages 53 and 70 were cultured in plastic dishes (100 mm Falcon, Becton-Dickinson Labware, Mississauga, Ontario, Canada) at 37°C in an atmosphere of 95% air, 5% CO 2 , in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (CELLect Gold, ICN/Flow, Costa Mesa, CA), 4 mM glutamine, and 20 mM HEPES and cultures were refed every 48 h.…”

Section: Methodsmentioning

confidence: 61%

“…Cell Culture-The human colon carcinoma Caco-2/15 cell line, a stable clone of the parent Caco-2 cell line (HBT 37; ATCC, Rockville, MD) has been characterized elsewhere (42)(43)(44). These cells are unique in that upon reaching confluence they spontaneously undergo a gradual enterocytic differentiation process, similar to that observed in the epithelium of the intact fetal small and large intestine (42)(43)(44)(45).…”

Section: Methodsmentioning

confidence: 99%

“…To further investigate this, we used two well characterized intestinal cell models which allow to some extent the recapitulation of the crypt-villus axis in vitro: the crypt-like HIEC cells, which are proliferative and undifferentiated (41), and the Caco-2/15 cells which have the ability to differentiate into fully functional villus-like enterocytes (42)(43)(44). Our data show that differentiated enterocytes express a full-length 205-kDa ␤ 4 A subunit while undifferentiated crypt cells express a novel ␤ 4 A subunit which does not contain the COOH-terminal segment of the cytoplasmic domain (␤ 4 A ctdϪ ).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Expression of Functionally Distinct Variants of the β4A Integrin Subunit in Relation to the Differentiation State in Human Intestinal Cells

Basora¹,

Herring-Gillam

Boudreau³

et al. 1999

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Integrins are important mediators of cell-laminin interactions. In the small intestinal epithelium, which consists of spatially separated proliferative and differentiated cell populations located, respectively, in the crypt and on the villus, laminins and laminin-binding integrins are differentially expressed along the cryptvillus axis. One exception to this is the integrin ␣ 6 ␤ 4 , which is thought to be ubiquitously expressed by intestinal cells. However, in this study, a re-evaluation of the ␤ 4 subunit expression with different antibodies revealed that two forms of ␤ 4 exist in the human intestinal epithelium. Furthermore, we show that differentiated enterocytes express a full-length 205-kDa ␤ 4 A subunit, whereas undifferentiated crypt cells express a novel ␤ 4 A subunit that does not contain the COOH-terminal segment of the cytoplasmic domain (␤ 4 A ctd؊ ). This new form was not found to arise from alternative ␤ 4 mRNA splicing. Moreover, we found that these two ␤ 4 A forms can associate into ␣ 6 ␤ 4 A complexes; however, the ␤ 4 A ctd؊ integrin expressed by the undifferentiated crypt cells is not functional for adhesion to laminin-5. Hence, these studies identify a novel ␣ 6 ␤ 4 A ctd؊ integrin expressed in undifferentiated intestinal crypt cells that is functionally distinct.

show abstract

Section: Methodsmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Expression of Functionally Distinct Variants of the β4A Integrin Subunit in Relation to the Differentiation State in Human Intestinal Cells

Basora¹,

Herring-Gillam

Boudreau³

et al. 1999

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thermolysin contributed to the isolation of epithelial cells from the lung and trachea, and of keratinocytes in the past (20,26). In recent years, it has also been applied to the isolation of many other highyield homogenous and viable cells (27).…”

Section: Discussionmentioning

confidence: 99%

“…The human colon carcinoma cell line Caco-2 is a clone of the parent Caco-2 cell line (HBT-37; ATCC, China), which has been characterized elsewhere, with its culture conditions having been defined (20). Cells were used between passages 60 and 80.…”

Section: Caco-2 and Human Intestinal Mesenchymal Cell Culturesmentioning

confidence: 99%

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

Liu

Zhang

Zhou

et al. 2010

Braz J Med Biol Res

View full text Add to dashboard Cite

Epithelium, a highly dynamic system, plays a key role in the homeostasis of the intestine. However, thus far a human intestinal epithelial cell line has not been established in many countries. Fetal tissue was selected to generate viable cell cultures for its sterile condition, effective generation, and differentiated character. The purpose of the present study was to culture human intestinal epithelial cells by a relatively simple method. Thermolysin was added to improve the yield of epithelial cells, while endothelin-3 was added to stimulate their growth. By adding endothelin-3, the achievement ratio (viable cell cultures/total cultures) was enhanced to 60% of a total of 10 cultures (initiated from 8 distinct fetal small intestines), allowing the generation of viable epithelial cell cultures. Western blot, real-time PCR and immunofluorescent staining showed that cytokeratins 8, 18 and mouse intestinal mucosa-1/39 had high expression levels in human intestinal epithelial cells. Differentiated markers such as sucrase-isomaltase, aminopeptidase N and dipeptidylpeptidase IV also showed high expression levels in human intestinal epithelial cells. Differentiated human intestinal epithelial cells, with the expression of surface markers (cytokeratins 8, 18 and mouse intestinal mucosa-1/39) and secretion of cytokines (sucrase-isomaltase, aminopeptidase N and dipeptidylpeptidase IV), may be cultured by the thermolysin and endothelin-3 method and maintained for at least 20 passages. This is relatively simple, requiring no sophisticated techniques or instruments, and may have a number of varied applications.

show abstract

Human cell models to study small intestinal functions: Recapitulation of the crypt-villus axis

Pageot

Perreault

Basora

et al. 2000

Microsc. Res. Tech.

124

View full text Add to dashboard Cite

Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line

Cited by 200 publications

References 39 publications

Expression of Functionally Distinct Variants of the β4A Integrin Subunit in Relation to the Differentiation State in Human Intestinal Cells

Expression of Functionally Distinct Variants of the β4A Integrin Subunit in Relation to the Differentiation State in Human Intestinal Cells

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

Human cell models to study small intestinal functions: Recapitulation of the crypt-villus axis

Contact Info

Product

Resources

About