Transient Human Monoclonal Immunoglobulins

Danon, F; Séligmann, M

doi:10.1111/j.1365-3083.1972.tb03297.x

Cited by 19 publications

(5 citation statements)

References 21 publications

(8 reference statements)

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“…The monoclonal proliferation of IgA producing cells may reflect a partial defect of the IgA production which is 'compensated' for by increased production of monoclonal IgA. This hypothesis is in line with the common findings of monoclonal Ig in patients with immuno-deficiencies, such as hypogammaglobulinaemia (Axelsson et a1 1966, Danon & Seligmann 1972) and Wiskott-Aldrich's syndrome (Danon & Seligmann 1972).…”

Section: Discussionsupporting

confidence: 53%

Familial IgA Defects

Beermann¹,

Holm²

1974

Scandinavian Journal of Haematology

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Previous reports indicate that deficiency of IgA is sometimes under genetical control. Dominant, recessive and autosomal modes of inheritance have been suggested. This paper describes a family with abnormal levels of IgA in three generations. There was one male with benign monoclonal IgA‐λ‐elevation in the first generation. The daughter and two of her three children (one male and one female) had IgA deficiency. In addition, two of these subjects had subnormal levels of IgE. One of the IgA (and IgE) deficient individuals had chronic urticaria, whereas the other two were healthy.

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Section: Discussionsupporting

confidence: 53%

Familial IgA Defects

Beermann¹,

Holm²

1974

Scandinavian Journal of Haematology

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“…Our results suggest that eAg is an immunoglobulin with restricted heterogeneity. Such immunoglobulins may be formed as a consequence of repeated stimulation with th ae antigen (31), as in the case of chronic infections (32,33 (32). In this respect, it is of interest that hepatitis B may be associated with the synthesis of transient monoclonal immunoglobulins (32,34).…”

Section: Discussionmentioning

confidence: 99%

Host specificity of a serum marker for hepatitis B: evidence that "e antigen" has the properties of an immunoglobulin.

Neurath¹,

Strick²

1977

Proc. Natl. Acad. Sci. U.S.A.

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A family of antigens, referred to collectively as e antigen (eAg), has been detected in sera of some individuals with liver disease who test positive for hepatitis B surface antigen. Studies on eAg partially purified by affinity chromatography on insolubilized antiboies to eAg revealed the following: (i) (eAg). Though distinct from HBsAg in both immunologic and physicochemical properties (8), eAg is also specifically associated with HBV infections and appears to be a marker for the infectivity of HBsAg-positive sera (7, 9, 10); its persistence seems to be associated with chronic liver disease (11). Our attempts to characterize eAg, described here, led to the surprising finding that eAg has the properties of an immunoglobulin. MATERIALS AND METHODSeAg was partially purified by precipitation with polyethylene glycol 6000, followed either by affinity chromatography on columns of insolubilized antibodies to eAg (eAbl (12) or by chromatography on DEAE-Bio-Gel (13). eAb was prepared from human sera by sequential precipitation with Rivanol and Na2SO4 (14). Antibodies to individual human plasma proteins and to IgG subclasses were each linked to Sepharose 4B as described (15). Normal human serum or IgG was insolubilized by the same procedure.Partially purified eAg (total amount of protein: 90-250 mg in 30 mM sodium citrate, pH 7.4) was submitted to isoelectric focusing (16). Fractions after isoelectric focusing were adjusted to pH 7, dialyzed against 0.14 M NaCl/0.01 M Tris-HCI at pH 7.2 (Tris/saline), concentrated about 10-fold, and tested for eAg. Identical results were obtained with either the cathode or the anode positioned in the bottom of the electrofocusing column.To compare the sedimentation rates of eAg and an IgG (18), respectively. The F(ab')2 fragments were isolated by chromatography on Sephadex G-150 (17). The Fab fragments were separated from Fc fragments by affinity chromatography on anti-Fab columns. F(ab")2 fragments from samples containing eAg were prepared by cleavage with CNBr (19), followed by affinity chromatography on anti-Fab columns.eAg purified by affinity chromatography or proteins eluted with 50 mM Na2B407 (pH 10.9) from extensively washed eAg-eAb precipitin lines excised from the agarose gel of rheophoresis plates were each labeled with 1251 (20) or with [125I]-iodinated p-hydroxyphenylpropionic acid N-hydroxysuccinimide ester (Bolton-Hunter reagent; New England Nuclear, Boston, MA). Similar results were obtained by either of the two methods. Labeled eAg was further purified by isoelectric focusing. Fractions collected in the pH range of 4.9-5.7 were pooled, dialyzed against Tris/saline, mixed with 2 ml of lamb serum and with normal human serum proteins, precipitated from 10 ml of serum with polyethylene glycol 6000 (600 mg), and resuspended in 2 ml of Tris/saline. Labeled eAg from this mixture was repurified by affinity chromatography on a 15-ml column of insolubilized eAb.The method for polyacrylamide gel electrophoresis described earlier (16) was used. Samples were dissociated...

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“…Not only may this reveal an unchanging paraprotein concentration but, in some subjects, the paraproteinaemia will be found to be transient, a phenomenon recorded in association with a heterogeneous assortment of diseases, including acute and chronic infections, collagen diseases, cirrhosis, genetically determined immune deficiency states, successfully treated non-reticular neoplasms, prosthetic heart valve insertion, and other conditions, including malaria, folic acid deficiency, gastric ulcer, and cold sensitivity (Young, 1969;Cooke, 1969;Danon and Seligmann, 1972). The significance of transient paraproteinaemia is not yet clear, but in some cases it represents a monoclonal antibody response to an antigen.…”

Section: Managementmentioning

confidence: 99%

“…It may be postulated that a paraprotein represents a specific response to a particular antigen (Metzger, 1969;Potter, 1971; Danon and Seligmann, 1972) which could be either bacterial or viral (Mitchell, Rees, and Salisbury, 1971). However, the number of cases in which the paraprotein has been shown to possess specific antibody activity is relatively small, and of these the majority appear to be autoimmune antibodies.…”

Section: Pathogenesis and Aetiologymentioning

confidence: 99%

Benign paraproteinaemias.

Kohn¹

1975

Journal of Clinical Pathology

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Transient Human Monoclonal Immunoglobulins

Cited by 19 publications

References 21 publications

Familial IgA Defects

Familial IgA Defects

Host specificity of a serum marker for hepatitis B: evidence that "e antigen" has the properties of an immunoglobulin.

Benign paraproteinaemias.

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