Transient genetic transformation of <i>Mougeotia scalaris</i> (Zygnematophyceae) mediated by the endogenous α‐tubulin1 promoter

Regensdorff, Merle; Deckena, Marek; Stein, Maike; Borchers, Agnes; Scherer, Gwydion; Lammers, Maike; Hänsch, Robert; Zachgo, Sabine; Buschmann, Henrik

doi:10.1111/jpy.12781

Cited by 18 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serlin and Roux 1984;Wagner and Klein 1981;Wagner et al 1972 and references therein). Recently, a transient transformation system was established in Mougeotia scalaris (Regensdorff et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Cell wall characteristics during sexual reproduction of Mougeotia sp. (Zygnematophyceae) revealed by electron microscopy, glycan microarrays and RAMAN spectroscopy

et al. 2021

View full text Add to dashboard Cite

Mougeotia spp. collected from field samples were investigated for their conjugation morphology by light-, fluorescence-, scanning- and transmission electron microscopy. During a scalarifom conjugation, the extragametangial zygospores were initially surrounded by a thin cell wall that developed into a multi-layered zygospore wall. Maturing zygospores turned dark brown and were filled with storage compounds such as lipids and starch. While M. parvula had a smooth surface, M. disjuncta had a punctated surface structure and a prominent suture. The zygospore wall consisted of a polysaccharide rich endospore, followed by a thin layer with a lipid-like appaerance, a massive electron dense mesospore and a very thin exospore composed of polysaccharides. Glycan microarray analysis of zygospores of different developmental stages revealed the occurrence of pectins and hemicelluloses, mostly composed of homogalacturonan (HG), xyloglucans, xylans, arabino-galactan proteins and extensins. In situ localization by the probe OG7-13AF 488 labelled HG in young zygospore walls, vegetative filaments and most prominently in conjugation tubes and cross walls. Raman imaging showed the distribution of proteins, lipids, carbohydrates and aromatic components of the mature zygospore with a spatial resolution of ~ 250 nm. The carbohydrate nature of the endo- and exospore was confirmed and in-between an enrichment of lipids and aromatic components, probably algaenan or a sporopollenin-like material. Taken together, these results indicate that during zygospore formation, reorganizations of the cell walls occured, leading to a resistant and protective structure.

show abstract

Section: Introductionmentioning

confidence: 99%

Cell wall characteristics during sexual reproduction of Mougeotia sp. (Zygnematophyceae) revealed by electron microscopy, glycan microarrays and RAMAN spectroscopy

et al. 2021

View full text Add to dashboard Cite

show abstract

“…We thus honed in on the plastid-associated biology of Mougeotia . For this, we made use of the emerging model system M. scalaris SAG 164.80 (Regensdorff et al 2018 ; Figure 2A ) and investigated its photophysiological changes after submergence using PAM. For this, we used three experimental setups, each with a minimum of three replicates.…”

Section: Resultsmentioning

confidence: 99%

Submergence of the filamentous Zygnematophyceae Mougeotia induces differential gene expression patterns associated with core metabolism and photosynthesis

et al. 2021

View full text Add to dashboard Cite

The streptophyte algal class Zygnematophyceae is the closest algal sister lineage to land plants. In nature, Zygnematophyceae can grow in both terrestrial and freshwater habitats and how they do this is an important unanswered question. Here, we studied what happens to the zygnematophyceaen alga Mougeotia sp., which usually occurs in permanent and temporary freshwater bodies, when it is shifted to liquid growth conditions after growth on a solid substrate. Using global differential gene expression profiling, we identified changes in the core metabolism of the organism interlinked with photosynthesis; the latter went hand in hand with measurable impact on the photophysiology as assessed via pulse amplitude modulation (PAM) fluorometry. Our data reveal a pronounced change in the overall physiology of the alga after submergence and pinpoint candidate genes that play a role. These results provide insight into the importance of photophysiological readjustment when filamentous Zygnematophyceae transition between terrestrial and aquatic habitats.

show abstract

“…Molecular and genetic analysis of such species will likely bring such insights. With an increasing number of genomes becoming available in this group (Nishiyama et al 2018 ; Cheng et al 2019 ; Jiao et al 2020 ) and with reports of genetic transformation (Abe et al 2011 ; Sørensen et al 2014 ; Regensdorff et al 2018 ), the roots of this most fundamental of processes in plant sexual reproduction will hopefully soon be explored.…”

Section: The Formation Of Haploid Gametes and Fertilizationmentioning

confidence: 99%

Flowering plant embryos: How did we end up here?

Rensing

Weijers

2021

Plant Reprod

View full text Add to dashboard Cite

The seeds of flowering plants are sexually produced propagules that ensure dispersal and resilience of the next generation. Seeds harbor embryos, three dimensional structures that are often miniatures of the adult plant in terms of general structure and primordial organs. In addition, embryos contain the meristems that give rise to post-embryonically generated structures. However common, flowering plant embryos are an evolutionary derived state. Flowering plants are part of a much larger group of embryo-bearing plants, aptly termed Embryophyta. A key question is what evolutionary trajectory led to the emergence of flowering plant embryos. In this opinion, we deconstruct the flowering plant embryo and describe the current state of knowledge of embryos in other plant lineages. While we are far yet from understanding the ancestral state of plant embryogenesis, we argue what current knowledge may suggest and how the knowledge gaps may be closed.

show abstract

Transient genetic transformation of Mougeotia scalaris (Zygnematophyceae) mediated by the endogenous α‐tubulin1 promoter

Cited by 18 publications

References 44 publications

Cell wall characteristics during sexual reproduction of Mougeotia sp. (Zygnematophyceae) revealed by electron microscopy, glycan microarrays and RAMAN spectroscopy

Cell wall characteristics during sexual reproduction of Mougeotia sp. (Zygnematophyceae) revealed by electron microscopy, glycan microarrays and RAMAN spectroscopy

Submergence of the filamentous Zygnematophyceae Mougeotia induces differential gene expression patterns associated with core metabolism and photosynthesis

Flowering plant embryos: How did we end up here?

Contact Info

Product

Resources

About