Transient gene expression in shoot apical meristems of sugarbeet seedlings after particle bombardment

Abstract

Sugarbeet apices were used as targets for particle bombardment with a microtargeting device. Before examining gene expression, particle penetration experiments were carried out. Transient GUS expression was detected within the first and second cell layers of the meristem. Dividing cells with GUS activity demonstrated that cells survived the bombardment procedure.

Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica)

“…(1) pFF19G (Timmermans et al, 1990), which contains the uidA gene controlled by the CaMV35S promoter; (2) pNG (Mahn et al, 1995) which contains the uidA and nptII genes both under the control of the CaMV35S promoter and (3) pPARGUSH (Landsmann et al, 1988), which contains the nptII gene under the control of the nos promoter and the uid A gene under the control of the par promoter. The DNA coated particles were prepared as previsously described (Sanford et al 1993) with modifications (Mahn, 1996).…”

“…The biolistic method, which bypasses the problems associated with Agrobacterium host range, offers an alternative approach for the delivery of DNA into plant cells. It has been used in transient and/or integrative transformation of many plants, such as rice (Jain et al, 1996), cassava (Schopke et al, 1996), sugarbeet (Mahn et al, 1995) and black spruce (Charest et al, 1996). This is the first demonstration that this technique can be used to express a transgene in a member of the Cactaceae.…”

Factors affecting somatic embryogenesis and transformation in modern plant breeding

“…tissue for high frequency recovery of non-chimeric transgenic plants (Taylor and Fauquet, 2002). Such cultures possess a high proportion of cytoplasmically rich, actively dividing cells, which provide high levels of transient expression and high frequency transgene integration (Mahn et al, 1995). In addition, it is believed that these cells are better able to overcome the stress induced by transformation (Santos et al, 2002).…”

Construction of Plant Transformation Vectors Carrying Beet Necrotic Yellow Vein Virus Coat Protein Gene (II)- Plant Transformation

“…No matter what type of explant was used (16,21,34), the induction of transgenic plants was very low and strongly dependant on genotype or binary vector. Particle bombardment of apical meristem (22), cell suspension (15) and embryogenic callus (31) did not give better results. The only transformation method that gave stable results regardless of genotype or vector is PEG transformation of stomatal guard cells protoplasts (12,30), but this method is technically demanding and expensive.…”