Transient Gene Expression in Intact and Organized Rice Tissues.

Science Behind Cotton Transformation

“…It was reported that the best results were obtained for maize. Researchers transformed immature embryos and embryogenic callus type I, which were treated by a solution of pectolytic enzymes, and then transferred into electroporating cuvettes [66]. The electroporation efficiency was relatively high when compared with micro bombardment conducted for same species, and about 90 transgenic plants were regenerated from 1440 embryos (6.25%) and 31 plants from 55 callus clusters (54.6%).…”

“…Pena et al [65] used this protocol for transformation in rye. Limitation of pollen tube pathway method reported by Shou et al [66] collected pollens from the genetically engineered plants and reported 10-fold less efficiency in soybean. It was concluded that pollen tube pathway is not reproducible in case of cotton and soybean ( Figure 4).…”

“…Major limitations in this technique are its low efficiency and being so hectic. Therefore, success story for liposome-mediated transformation have been published so far only for tobacco [66] and wheat [67].…”

Messenger DNA in higher plants

“…Such DNA was found to be transcribed in the host plant cells. 3,13 Direct uptake of plasmid Pl3l 221.2 carrying the GUS gene DNA by imbibition of dry somatic embryos of Medicago sativa has been reported, 14 whilst Dekeyser et al 15 also show the uptake and expression of DNA by rice. The feeding of cut shoots of L. esculentum with […”

Genetic Engineering of Plants for Resistance to Viruses

“…Liposomes can also be carried through the pores of pollen grains to fuse with the membrane of the pollen grain. Transgenic plants have been reported by liposome-mediated transformation only from tobacco (Dekeyser et al, 1990) and wheat (Zhu et al, 1993). The process is inexpensive, but is laborious and inefficient, and so has not been widely adopted.…”