Transient Expression of CC Chemokine TECK in the Ovary during Ovulation: Its Potential Role in Ovulation

Zhou, Cindy; Wu, Jean; Borillo, Jason; Torres, Lisa; McMahon, John; Bao, Yongde; Lou, Yahuan

doi:10.1111/j.1600-0897.2005.00265.x

Cited by 23 publications

(20 citation statements)

References 37 publications

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“…The assembled sets were incubated at 37°C, 5% CO 2 , for 8 h, and the cells in the insert wells were gently and thoroughly washed away with cold PBS. After fixation in 2% PFA for 10 min at 4°C, the membrane was stained by anti-CD8 Ab following an established immunoperoxidase staining method (23). In some cases, bioactive TECK (R&D Systems) was added to the lower chamber (10 g/ml) as chemoattractant.…”

Section: Leukocyte Infiltration Assaymentioning

confidence: 99%

“…Chemotactic activity of ovaries undergoing the ovulatory process for PBL was measured by leukocyte infiltration assay following our previously published method (23). In brief, the ovaries collected from mice at 8 h post hCG injection were teased open, creating a chemoattractant source, and placed at the lower chamber of a BD Bio Coat Control Cell Culture Inserts system (pore size 3 , BD Biosciences).…”

Section: Leukocyte Infiltration Assaymentioning

confidence: 99%

“…Dead cells were further removed by Ficoll gradient (density 1.119) centrifugation. As a control, peripheral blood leukocytes or splenocytes were isolated following our previously published method (23). The cells were doubly stained by various combinations of two Abs and analyzed by flow cytometry (FACSCalibur, BD Biosciences).…”

Section: Isolation Of Ovarian Cells and Flow Cytometrymentioning

confidence: 99%

“…Recombinant mouse TECK (rmTECK) was expressed and purified as described previously (23), adjusted to 3 mg/ml, and was emulsified in an equal volume of CFA. Each mouse received 0.1 ml of the mixture (150 g of rmTECK) in one footpad and a s.c. site.…”

Section: Immunization and Fertility Trailmentioning

confidence: 99%

“…However, a recent study has shown elevated expression of different chemokines (mainly CC type MCP-1 and RANTES) during the ovulatory process, suggesting their potential roles in ovulation (20,21). Recently, we have detected a transient expression of a CC chemokine thymus-expressed chemokine (TECK) 3 and its receptor CCR9 during human chorionic gonadotropin (hCG)-induced ovulation (22,23). However, it still remains to be determined whether these chemokines are indeed involved in ovulation and which specific leukocyte populations these chemokines recruit.…”

mentioning

confidence: 99%

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Potential Roles of a Special CD8αα+ Cell Population and CC Chemokine Thymus-Expressed Chemokine in Ovulation Related Inflammation

Zhou

Wu²,

Borillo³

et al. 2009

The Journal of Immunology

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It is well known that ovulation may be an inflammatory process. However, it remains elusive how immune cells participate in this process. We have identified a novel CD8αα+ population, which resembles tissue dendritic cells, in the theca of antral follicles. We further observed a dramatic influx of the CD8αα+cells into the ovulating follicles. This CD8αα+population was absent in the ovary of estradiol-induced anovulatory C31F1 mice and subfertile athymic nude mice. Expression of a CC chemokine thymus-expressed chemokine (TECK) has previously been found in the ovary; we further demonstrated that TECK attracted CD8αα+cells into the ovary. Anti-TECK Ab, elicited in the female mice by active immunization, depleted the ovarian CD8αα+ cells in vivo. Mice with a high titer of TECK Ab failed to ovulate after superovulation induction. More importantly, the immunized mice had greatly reduced fertility, which was positively correlated with the Ab titers. Ovarian TECK expression was normal in anovulatory C31F1 mice, suggesting that infertility in the immunized mice is due to a block of CD8αα+ cell migration. Finally, the origin of ovarian CD8αα+ cells was explored. Upon being transferred, thymic CD8α+ cells were able to home to the theca of follicles in the recipients. Thus, ovarian CD8αα+ cells, which participate in the ovulation-related inflammation, may originate in the thymus.

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Section: Leukocyte Infiltration Assaymentioning

confidence: 99%

Section: Leukocyte Infiltration Assaymentioning

confidence: 99%

Section: Isolation Of Ovarian Cells and Flow Cytometrymentioning

confidence: 99%

Section: Immunization and Fertility Trailmentioning

confidence: 99%

mentioning

confidence: 99%

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Potential Roles of a Special CD8αα+ Cell Population and CC Chemokine Thymus-Expressed Chemokine in Ovulation Related Inflammation

Zhou

Wu²,

Borillo³

et al. 2009

The Journal of Immunology

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Follicle populations and gene expression profiles of Nelore and Angus heifers with low and high ovarian follicle counts

Favoreto

Loureiro

Ereno

et al. 2018

Molecular Reproduction Devel

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The number of antral follicles counted (AFC) by ultrasound is associated with fertility in cattle. Cows with higher follicle count (HFC) have higher performance in reproductive‐assisted technologies than cows with lower follicle count (LFC). In this study, we aimed to define the preantral follicle count by histology and to identify differentially expressed genes (DEGs) using a microarray in Nelore and Angus heifers with HFC and LFC. The ovaries of each animal were scanned with an ultrasound device 12 to 24 hr after estrus. The groups were formed based on the average number of total follicles (≥3 mm) counted in each breed consistently ± the standard deviation. For the histological analysis, preantral follicles were counted and classified under a stereo microscope, and follicle density was determined. Microarray analysis was performed on pools of three follicles dissected from the ovaries of 15 Nelore (6 HFC and 9 LFC) and 17 Angus heifers (9 HFC and 8 LFC). Angus heifers have increased total and primordial follicle density. Nelore heifers have increased antral follicle count. Different patterns of gene expression regulate follicle recruitment and development in Angus and Nelore heifers and may be associated with the different follicle densities observed in Angus versus Nelore heifers. Furthermore, HFC heifers presented increased expression of genes associated with cellular development and metabolism.

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Ovulation: A Molecular View

Brännström

Lind

Dahm‐Kähler

2010

Reproductive Endocrinology and Infertility

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Transient Expression of CC Chemokine TECK in the Ovary during Ovulation: Its Potential Role in Ovulation

Abstract: This study suggested that ovarian expression of TECK is under a tight hormonal regulation, and expressed TECK may be responsible for recruitment of mononuclear cells into the ovary to participate in the ovulatory process.

Cited by 23 publications

References 37 publications

Potential Roles of a Special CD8αα+ Cell Population and CC Chemokine Thymus-Expressed Chemokine in Ovulation Related Inflammation

Potential Roles of a Special CD8αα+ Cell Population and CC Chemokine Thymus-Expressed Chemokine in Ovulation Related Inflammation

Follicle populations and gene expression profiles of Nelore and Angus heifers with low and high ovarian follicle counts

Ovulation: A Molecular View

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