Transient decrease in human peripheral blood myeloid dendritic cells following influenza vaccination correlates with induction of serum antibody

Abstract

Dendritic cells (DC) are critical inducers of the adaptive immune response. Extensive characterization of tissue-resident and monocyte-derived DC has revealed diverse stimulatory and regulatory actions, although the role of peripheral blood dendritic cells (PBDC) in maintaining homeostasis remains unclear. Examination of various myeloid (CD11c+CD303−) and plasmacytoid (CD11c−CD303+) DC populations in the peripheral blood of seasonal trivalent inactivated influenza vaccine recipients revealed a transient decrease in the frequency of CD11c+CD1c− myeloid DC subsets 5–10 days following vaccination, including both CD141+ and CD141− myeloid DC subsets of this population. These populations rebounded by 1 month, while plasmacytoid DC remained stable. The magnitude of the decrease in the CD141+ myeloid DC subset at d5–7 significantly correlated with the induction of influenza specific serum antibodies measured at 1 month following vaccination. These results demonstrate a mobilization of peripheral blood myeloid DC following vaccination and indicate these cells are potential biomarkers of immune response.

Altered Immune Regulation of Dendritic Cells and Enhanced Cytokine Production of T Cells in the Pathogenesis of Eosinophilic Chronic Rhinosinusitis

“…The endocytic capacity of DCs was determined by calculating the ΔMFI (MFI of cells incubated at 4°C subtracted from MFI of cells incubated at 37°C). The doses of FITC-dextran particles and Lucifer yellow CH potassium salt were based on previous reports [17][18][19][20]. The 2 stains were washed out with cold staining buffer after incubation, according to the previously described method [21].…”