Transient characteristics of universal cells on human‐induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources

Sung, Tzu Cheng; Jiang, Yuanchun; Hsu, Jhe Yu; Ling, Qing; Chen, Hao; Kumar, Suresh; Chang, Yung; Hsu, Shih Tien; Ye, Qingsong; Higuchi, Akon

doi:10.1111/cpr.12995

Cited by 7 publications

(6 citation statements)

References 47 publications

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“…hESCs (H9) were obtained from the WiCell Research Institute (Madison, WI, USA). hiPSCs (HM-5) reprogrammed from amniotic fluid stem cells using the Sendai virus vector in our previous study 26 were also used in this study. The other reagents and biomaterials utilized in this project are presented in Table S1 (ESI†).…”

Section: Methodsmentioning

confidence: 99%

Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

et al. 2022

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“…The immunostaining of hESCs for pluripotent proteins Oct3/4, SSEA‐4, Nanog and Sox2 was performed to study the pluripotency of hESCs following a conventional assay [ 11 , 27 , 28 , 29 , 30 ]. The expression levels of Oct3/4, SSEA‐4, Nanog and Sox2 as well as the nuclei staining results using Hoechst were evaluated under a fluorescence microscope (Eclipse Ti‐U, Nikon Instruments, Inc., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…After two weeks of cultivation in suspension, EBs were transferred to a Matrigel‐coated surface, where the cells were incubated for an additional month. Then, the cells were stained with antibodies against all three markers of embryonic germline layers (AFP (endoderm, α ‐fetoprotein), SMA (mesoderm, smooth muscle actin) and GFAP (ectoderm, glial fibrillary acidic protein)) by applying a conventional assay, and the cells were observed under a fluorescence microscope [ 11 , 27 , 28 , 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

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Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments

Alarfaj

Hirad

Munusamy

et al. 2022

IET Nanobiotechnology

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Human pluripotent stem cells (hPSCs) can be proliferated on completely synthetic materials under xeno‐free cultivation conditions using biomaterials grafted with extracellular matrix protein (ECM)‐derived peptides. However, cell culture biomaterials grafted with ECM‐derived peptides must be prepared using a high concentration of peptide reaction solution (e.g. 1000 μg/ml), whereas the ECM concentration of the ECM‐coated surface for hPSC culture is typically 5 μg/ml. We designed a polyethylene glycol (PEG) joint nanosegment (linker) to be used between base cell culture biomaterials and bioactive ECM‐derived peptides to enhance the probability of contact between ECM‐derived peptides and cell binding receptors of hPSCs. Vitronectin‐derived peptides with glycine joint nanosegments (GCGG) were conjugated onto poly (vinyl alcohol‐co‐itaconic acid) hydrogels via PEG joint nanosegments, and human embryonic stem cells (hESCs) were cultivated on these hydrogels. hESCs could successfully be cultivated on hydrogels while maintaining their pluripotency and differentiation potential to differentiate into cells that are induced from three germ layers in vitro and in vivo, where only a 50 μg/ml ECM‐derived peptide concentration was used when the PEG joint nanosegments were introduced into peptides that were grafted onto hydrogel surfaces. The joint nanosegments between bioactive peptides and base cell culture biomaterials were found to contribute to efficient hESC attachment and proliferation.

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“…Stimulating a patient's endogenous stem cells to divide or differentiate, which occurs naturally when skin wounds heal, is a common alternative to somatic cell transplantation. Fibroblasts are the best stem cell source because they can be stimulated quickly and with more precision (Sung et al, 2021).…”

Section: Sources Of Ipscsmentioning

confidence: 99%

Stem Cells and Its Multimodality - A Review

2022

pnr

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Stem cells are unique human cells that have the ability to grow rapidly, usually from a single cell, and differentiate into many cell and tissue types. It can differentiate into any sort of cell, which can then differentiate into daughter cells, which can then differentiate into hematopoietic stem cells. To avoid the creation of teratomas, hESCs are derived from traditional culture to laser-assisted methods or microsurgery, and their differentiation must be determined. Inner cell mass (ICM) and trophectoderm are the two cell types that make up blastocysts (TE). Pluripotent cells have the ability to proliferate and differentiate into any type of cell, and they can be a limitless source of cells. In stem cell therapy, iPSCs eliminate the requirement for embryos.The self-renewal property of ESC, Hwang et al. (Hwang, Varghese and Elisseeff, 2008) the optimum culture method for hESC-based cell and tissue treatment would be a defined culture free, according to the researchers. Certain diseases that are currently incurable, such as neurodegenerative diseases, may be able to be treated with stem cell therapy. We are better able to extend human life than at any other point in history thanks to stem cell therapy and all of its restorative effects. This review paper on stem cells and their control, together with insightful remarks on clinical application and future outcomes, gives stem cell renewal a lot of optimism.

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Transient characteristics of universal cells on human‐induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 7 publications

References 47 publications

Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments

Stem Cells and Its Multimodality - A Review

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